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Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus

Effect of oxygen tension, pH, and IL-1β on reactive oxygen species levels in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ROS levels were measured using the fluorescent probe DCF-DA. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
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f0015: Effect of oxygen tension, pH, and IL-1β on reactive oxygen species levels in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ROS levels were measured using the fluorescent probe DCF-DA. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.

Mentions: ROS levels were reduced in very low oxygen levels (<1% O2, pH 7.2) (P=0.02) but increased under acidic conditions (pH 6.2) (P=0.04) (Fig. 3A and B). ROS levels were significantly increased in the presence of IL-1β under all oxygen conditions (Fig. 3C and D). The reduction in ROS levels present under very low oxygen conditions was prevented by the presence of resveratrol and similarly resveratrol prevented the acid-induced increase in ROS. NAC did not appear to affect ROS levels under low oxygen conditions but muted the acid-induced rise when incubated in 2%O2.Both resveratrol and NAC reduced the IL-1β induced increase in ROS levels.


Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Effect of oxygen tension, pH, and IL-1β on reactive oxygen species levels in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ROS levels were measured using the fluorescent probe DCF-DA. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562226&req=5

f0015: Effect of oxygen tension, pH, and IL-1β on reactive oxygen species levels in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ROS levels were measured using the fluorescent probe DCF-DA. Bar charts represent mean±SEM, n=3. *P<0.05; †P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
Mentions: ROS levels were reduced in very low oxygen levels (<1% O2, pH 7.2) (P=0.02) but increased under acidic conditions (pH 6.2) (P=0.04) (Fig. 3A and B). ROS levels were significantly increased in the presence of IL-1β under all oxygen conditions (Fig. 3C and D). The reduction in ROS levels present under very low oxygen conditions was prevented by the presence of resveratrol and similarly resveratrol prevented the acid-induced increase in ROS. NAC did not appear to affect ROS levels under low oxygen conditions but muted the acid-induced rise when incubated in 2%O2.Both resveratrol and NAC reduced the IL-1β induced increase in ROS levels.

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus