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Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus

Effect of oxygen tension, pH, and IL-1β on mitochondrial membrane potential (ΔΨm) in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ΔΨm was measured using the fluorescent probe JC-1. Bar charts represent mean±SEM, n=3. *P<0.05;†P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
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f0010: Effect of oxygen tension, pH, and IL-1β on mitochondrial membrane potential (ΔΨm) in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ΔΨm was measured using the fluorescent probe JC-1. Bar charts represent mean±SEM, n=3. *P<0.05;†P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.

Mentions: Acidosis (pH 6.2) and reduced oxygen levels (<1% O2) led to significant mitochondrial membrane depolarization(Fig. 2A and B). In the presence of resveratrol in acidosis (pH 6.2) there was less depolarization,although levels were still below control conditions.Additionally, under very low oxygen conditions (<1% O2) ΔΨm increased from 27±2 to 65±9% in the presence of resveratrol (P =0.004). NAC similarly reduced hypoxic-dependent depolarization by significantly increasing ΔΨm under acidic conditions (at <1, 2, and 5% O2, pH 7.2) but had no effect at 21% O2. At very low oxygen levels (<1% O2, pH 7.2),N-acetylcysteine had an effect similarto that of resveratrol with ΔΨm levels increased to 56±5% of control values (P= 0.002).IL-1β had a pronounced effect on mitochondrial membrane potential, irrespective of oxygen level or pH (Fig. 2C and D). Both resveratrol and NAC reduced the level of mitochondrial membrane depolarization caused by IL-1β but levels still remained below control values.


Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Effect of oxygen tension, pH, and IL-1β on mitochondrial membrane potential (ΔΨm) in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ΔΨm was measured using the fluorescent probe JC-1. Bar charts represent mean±SEM, n=3. *P<0.05;†P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562226&req=5

f0010: Effect of oxygen tension, pH, and IL-1β on mitochondrial membrane potential (ΔΨm) in equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). ΔΨm was measured using the fluorescent probe JC-1. Bar charts represent mean±SEM, n=3. *P<0.05;†P<0.01 versus control (time=0, 5%O2, pH 7.2) or between groups where shown.
Mentions: Acidosis (pH 6.2) and reduced oxygen levels (<1% O2) led to significant mitochondrial membrane depolarization(Fig. 2A and B). In the presence of resveratrol in acidosis (pH 6.2) there was less depolarization,although levels were still below control conditions.Additionally, under very low oxygen conditions (<1% O2) ΔΨm increased from 27±2 to 65±9% in the presence of resveratrol (P =0.004). NAC similarly reduced hypoxic-dependent depolarization by significantly increasing ΔΨm under acidic conditions (at <1, 2, and 5% O2, pH 7.2) but had no effect at 21% O2. At very low oxygen levels (<1% O2, pH 7.2),N-acetylcysteine had an effect similarto that of resveratrol with ΔΨm levels increased to 56±5% of control values (P= 0.002).IL-1β had a pronounced effect on mitochondrial membrane potential, irrespective of oxygen level or pH (Fig. 2C and D). Both resveratrol and NAC reduced the level of mitochondrial membrane depolarization caused by IL-1β but levels still remained below control values.

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus