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Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus

Effect of oxygen tension, pH, and IL-1β on glyosaminoglycan (GAG) release from equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GAG release was measured in media using the dimethylmethylene blue (DMMB) assay. Bar charts represent mean±SEM, n=3. *P<0.05 versus control (time=0, 5%O2, pH 7.2).
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f0005: Effect of oxygen tension, pH, and IL-1β on glyosaminoglycan (GAG) release from equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GAG release was measured in media using the dimethylmethylene blue (DMMB) assay. Bar charts represent mean±SEM, n=3. *P<0.05 versus control (time=0, 5%O2, pH 7.2).

Mentions: Western blot analysis showed no change in expression of COL2A1 and SOX9 protein after the culture period, indicating maintenance of chondrocyte phenotype during this study (Fig. S1). Incubation in <1% O2 showed significant increase in LDH release, indicating reduced cell viability (Fig S2). In the presence of resveratrol (10 µM) or NAC (2 mM) this was reduced to control levels. Glycosaminoglycan release was significantly increased under acidic (pH 6.2) conditions in low oxygen (2% O2) in the presence of IL-1β(10 ng/ml) (136.2±4.8%, P= 0.001) (Fig. 1).Similarly, this increase was abatedwhen chondrocytes were incubated with resveratrol or NAC.


Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions.

Collins JA, Moots RJ, Clegg PD, Milner PI - Free Radic. Biol. Med. (2015)

Effect of oxygen tension, pH, and IL-1β on glyosaminoglycan (GAG) release from equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GAG release was measured in media using the dimethylmethylene blue (DMMB) assay. Bar charts represent mean±SEM, n=3. *P<0.05 versus control (time=0, 5%O2, pH 7.2).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4562226&req=5

f0005: Effect of oxygen tension, pH, and IL-1β on glyosaminoglycan (GAG) release from equine articular chondrocytes in the absence or presence of resveratrol or N-acetylcysteine. Equine articular chondrocytes were cultured in 3D alginate beads for 48 h in <1, 2, 5, or 21% O2 at pH 7.2 (A), pH 6.2 (B), pH 7.2 plus 10 ng/ml IL-1β (C) or pH 6.2 plus 10 ng/ml IL-1β (D) in the absence or presence of N-acetylcysteine (2 mM) or resveratrol (10 µM). GAG release was measured in media using the dimethylmethylene blue (DMMB) assay. Bar charts represent mean±SEM, n=3. *P<0.05 versus control (time=0, 5%O2, pH 7.2).
Mentions: Western blot analysis showed no change in expression of COL2A1 and SOX9 protein after the culture period, indicating maintenance of chondrocyte phenotype during this study (Fig. S1). Incubation in <1% O2 showed significant increase in LDH release, indicating reduced cell viability (Fig S2). In the presence of resveratrol (10 µM) or NAC (2 mM) this was reduced to control levels. Glycosaminoglycan release was significantly increased under acidic (pH 6.2) conditions in low oxygen (2% O2) in the presence of IL-1β(10 ng/ml) (136.2±4.8%, P= 0.001) (Fig. 1).Similarly, this increase was abatedwhen chondrocytes were incubated with resveratrol or NAC.

Bottom Line: In addition, SOD1 and SOD2 protein expressions were reduced.Both resveratrol and NAC partially restored ΔΨm and ROS levels and prevented GAG release and cell loss and normalized SOD1 and SOD2 protein expression.In particular NAC was highly effective at restoring the GSH:GSSG ratio.These results show that the antioxidants resveratrol and N-acetylcysteine can counteract the redox imbalance in articular chondrocytes induced by low oxygen and acidic conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst Campus, Cheshire, UK, CH64 7TE.

No MeSH data available.


Related in: MedlinePlus