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Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus

Differential potential and proliferative capacity of freshly isolated dental pulp stem cells (fresh-DPSCs) and cryopreserved dental pulp stem cells (cryo-DPSCs). a Adipogenic differentiation was observed by oil red O staining. Osteogenic differentiation was observed by alkaline phosphatase (ALP) activity. Bar = 250 μm. b The growth of fresh-DPSCs and cryo-DPSCs was measured as the cell number counted with a hemocytometer on day 3 and day 5 after seeding. c The proliferative activity of fresh-DPSCs and cryo-DPSCs was determined by the MTT assay on day 3 and day 5. Results are expressed as means ± SEM (n = 9)
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Fig7: Differential potential and proliferative capacity of freshly isolated dental pulp stem cells (fresh-DPSCs) and cryopreserved dental pulp stem cells (cryo-DPSCs). a Adipogenic differentiation was observed by oil red O staining. Osteogenic differentiation was observed by alkaline phosphatase (ALP) activity. Bar = 250 μm. b The growth of fresh-DPSCs and cryo-DPSCs was measured as the cell number counted with a hemocytometer on day 3 and day 5 after seeding. c The proliferative activity of fresh-DPSCs and cryo-DPSCs was determined by the MTT assay on day 3 and day 5. Results are expressed as means ± SEM (n = 9)

Mentions: We assessed the capacity of fresh-DPSCs and cryo-DPSCs to differentiate into adipocytes and osteoblasts. After adipogenic induction for 3 weeks, the cells were stained with oil red O for the detection of adipogenesis. Lipid cluster-positive cells were equally detected in both fresh-DPSCs and cryo-DPSCs (Fig. 7a). For the osteogenic induction, cells were cultured in the osteogenic induction medium for 2 weeks. ALP was identified at similar levels in fresh-DPSCs and cryo-DPSCs (Fig. 7a).Fig. 7


Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Differential potential and proliferative capacity of freshly isolated dental pulp stem cells (fresh-DPSCs) and cryopreserved dental pulp stem cells (cryo-DPSCs). a Adipogenic differentiation was observed by oil red O staining. Osteogenic differentiation was observed by alkaline phosphatase (ALP) activity. Bar = 250 μm. b The growth of fresh-DPSCs and cryo-DPSCs was measured as the cell number counted with a hemocytometer on day 3 and day 5 after seeding. c The proliferative activity of fresh-DPSCs and cryo-DPSCs was determined by the MTT assay on day 3 and day 5. Results are expressed as means ± SEM (n = 9)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4562193&req=5

Fig7: Differential potential and proliferative capacity of freshly isolated dental pulp stem cells (fresh-DPSCs) and cryopreserved dental pulp stem cells (cryo-DPSCs). a Adipogenic differentiation was observed by oil red O staining. Osteogenic differentiation was observed by alkaline phosphatase (ALP) activity. Bar = 250 μm. b The growth of fresh-DPSCs and cryo-DPSCs was measured as the cell number counted with a hemocytometer on day 3 and day 5 after seeding. c The proliferative activity of fresh-DPSCs and cryo-DPSCs was determined by the MTT assay on day 3 and day 5. Results are expressed as means ± SEM (n = 9)
Mentions: We assessed the capacity of fresh-DPSCs and cryo-DPSCs to differentiate into adipocytes and osteoblasts. After adipogenic induction for 3 weeks, the cells were stained with oil red O for the detection of adipogenesis. Lipid cluster-positive cells were equally detected in both fresh-DPSCs and cryo-DPSCs (Fig. 7a). For the osteogenic induction, cells were cultured in the osteogenic induction medium for 2 weeks. ALP was identified at similar levels in fresh-DPSCs and cryo-DPSCs (Fig. 7a).Fig. 7

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus