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Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus

Morphology and identification of cryo-DPSCs. a DPSCs, which were thawed after cryopreservation, were cultured and expanded. Cryo-DPSCs were of similar shape as the fresh-DPSCs observed with the phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of cryo-DPSCs. The expression of surface markers was analyzed with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies
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Fig6: Morphology and identification of cryo-DPSCs. a DPSCs, which were thawed after cryopreservation, were cultured and expanded. Cryo-DPSCs were of similar shape as the fresh-DPSCs observed with the phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of cryo-DPSCs. The expression of surface markers was analyzed with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies

Mentions: Cryo-DPSCs were thawed at 37 °C, re-cultured and expanded. The cryo-DPSCs showed the typical spindle-shaped morphology, which was the same as that of the fresh-DPSCs (Fig. 6a). The surface marker analysis revealed that cryo-DPSCs were positive for CD29, CD90 and CD49d and negative for CD34, CD45, which was the same pattern shown by the fresh-DPSCs (Fig. 6b).Fig. 6


Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Morphology and identification of cryo-DPSCs. a DPSCs, which were thawed after cryopreservation, were cultured and expanded. Cryo-DPSCs were of similar shape as the fresh-DPSCs observed with the phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of cryo-DPSCs. The expression of surface markers was analyzed with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4562193&req=5

Fig6: Morphology and identification of cryo-DPSCs. a DPSCs, which were thawed after cryopreservation, were cultured and expanded. Cryo-DPSCs were of similar shape as the fresh-DPSCs observed with the phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of cryo-DPSCs. The expression of surface markers was analyzed with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies
Mentions: Cryo-DPSCs were thawed at 37 °C, re-cultured and expanded. The cryo-DPSCs showed the typical spindle-shaped morphology, which was the same as that of the fresh-DPSCs (Fig. 6a). The surface marker analysis revealed that cryo-DPSCs were positive for CD29, CD90 and CD49d and negative for CD34, CD45, which was the same pattern shown by the fresh-DPSCs (Fig. 6b).Fig. 6

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus