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Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus

mRNA expressions of basic fibroblast growth factor (bFGF) vascular endothelial growth factor (VEGF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) in cultured dental pulp stem cells (DPSCs) and hindlimb skeletal muscles. a mRNA levels of these factors in cultured DPSCs were evaluated by RT-PCR. The products were visualized by agarose gel/ethidium bromide. b mRNA expressions of these factors in the hindlimb skeletal muscles were measured by RT-PCR. The relative quantity was calculated by the by the ΔΔCt method. Results are expressed as means ± SEM (n = 5)
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Fig5: mRNA expressions of basic fibroblast growth factor (bFGF) vascular endothelial growth factor (VEGF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) in cultured dental pulp stem cells (DPSCs) and hindlimb skeletal muscles. a mRNA levels of these factors in cultured DPSCs were evaluated by RT-PCR. The products were visualized by agarose gel/ethidium bromide. b mRNA expressions of these factors in the hindlimb skeletal muscles were measured by RT-PCR. The relative quantity was calculated by the by the ΔΔCt method. Results are expressed as means ± SEM (n = 5)

Mentions: To confirm whether cultured DPSCs express angiogenic and neurotrophic factors, we evaluated mRNA expression of VEGF, bFGF, NGF and NT-3 by RT-PCR. The products were visualized by agarose gel electrophoresis. As shown in Fig. 5a, DPSCs expressed all of these angiogenic and neurotrophic factors.Fig. 5


Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

mRNA expressions of basic fibroblast growth factor (bFGF) vascular endothelial growth factor (VEGF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) in cultured dental pulp stem cells (DPSCs) and hindlimb skeletal muscles. a mRNA levels of these factors in cultured DPSCs were evaluated by RT-PCR. The products were visualized by agarose gel/ethidium bromide. b mRNA expressions of these factors in the hindlimb skeletal muscles were measured by RT-PCR. The relative quantity was calculated by the by the ΔΔCt method. Results are expressed as means ± SEM (n = 5)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4562193&req=5

Fig5: mRNA expressions of basic fibroblast growth factor (bFGF) vascular endothelial growth factor (VEGF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) in cultured dental pulp stem cells (DPSCs) and hindlimb skeletal muscles. a mRNA levels of these factors in cultured DPSCs were evaluated by RT-PCR. The products were visualized by agarose gel/ethidium bromide. b mRNA expressions of these factors in the hindlimb skeletal muscles were measured by RT-PCR. The relative quantity was calculated by the by the ΔΔCt method. Results are expressed as means ± SEM (n = 5)
Mentions: To confirm whether cultured DPSCs express angiogenic and neurotrophic factors, we evaluated mRNA expression of VEGF, bFGF, NGF and NT-3 by RT-PCR. The products were visualized by agarose gel electrophoresis. As shown in Fig. 5a, DPSCs expressed all of these angiogenic and neurotrophic factors.Fig. 5

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus