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Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus

Morphology and characterization of fresh-DPSCs. a Cultured DPSCs observed with a phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of fresh-DPSCs. The expression of the surface markers was investigated with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies
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Fig1: Morphology and characterization of fresh-DPSCs. a Cultured DPSCs observed with a phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of fresh-DPSCs. The expression of the surface markers was investigated with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies

Mentions: Phase-contrast microscopic images of cultured DPSCs are shown in Fig. 1a. DPSCs were identified by their typical spindle-shape morphology. The surface markers of DPSCs showed high expression of CD29 and CD90, which are common stem cell markers in mesenchymal stem cells and DPSCs (Fig. 1b). DPSCs also showed relatively high expressions of CD49d, but lacked the expression of CD34 and CD45 [22]. The purity of DPSCs was achieved by passages and we found approximately 90 % purity after 3 passages. The differentiation ability of cultured DPSCs has already been confirmed in our previous paper [21].Fig. 1


Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.

Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K - Stem Cell Res Ther (2015)

Morphology and characterization of fresh-DPSCs. a Cultured DPSCs observed with a phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of fresh-DPSCs. The expression of the surface markers was investigated with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4562193&req=5

Fig1: Morphology and characterization of fresh-DPSCs. a Cultured DPSCs observed with a phase contrast microscope. Bar = 100 μm. b Flow cytometric analysis of fresh-DPSCs. The expression of the surface markers was investigated with CD29, CD34, CD49d, CD45 and CD90. Open histograms, isotype controls; filled histograms, stained with the specific surface marker antibodies
Mentions: Phase-contrast microscopic images of cultured DPSCs are shown in Fig. 1a. DPSCs were identified by their typical spindle-shape morphology. The surface markers of DPSCs showed high expression of CD29 and CD90, which are common stem cell markers in mesenchymal stem cells and DPSCs (Fig. 1b). DPSCs also showed relatively high expressions of CD49d, but lacked the expression of CD34 and CD45 [22]. The purity of DPSCs was achieved by passages and we found approximately 90 % purity after 3 passages. The differentiation ability of cultured DPSCs has already been confirmed in our previous paper [21].Fig. 1

Bottom Line: Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed.Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats.Cryopreservation of DPSCs did not impair their proliferative or differential ability.

View Article: PubMed Central - PubMed

Affiliation: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. hata@dpc.agu.ac.jp.

ABSTRACT

Introduction: Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy.

Methods: DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation.

Results: Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs.

Conclusions: We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy.

No MeSH data available.


Related in: MedlinePlus