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Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice.

Daifalla NS, Bayih AG, Gedamu L - J Immunol Res (2015)

Bottom Line: We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response.Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype.TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Calgary, Room 374, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4 ; The Forsyth Institute, Cambridge, MA 02142, USA.

ABSTRACT
We assessed the immune response against recombinant proteins of two related, albeit functionally different, peroxidoxins from Leishmania donovani: peroxidoxin 1 (LdPxn1) and peroxidoxin 2 (LdPxn2) in BALB/c mice. We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response. Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype. Antigen-stimulated spleen cells from mice that were immunized with rLdPxn1 produced low level of IL-10 and IL-4 and no IFN-γ, whereas cells from mice immunized with rLdPxn2 secreted high level of IFN-γ, low IL-4, and no IL-10. Coadministration of CpG ODN or GLA-SE with rLdPxn1 skewed the immune response towards a Th 1 type as indicated by robust production of IgG2a isotype. Furthermore, the presence of TLR agonists together with rLdPxn1 antigen enhanced the production of IFN-γ and to a lesser extent of IL-10. TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

No MeSH data available.


Cytokine responses in spleen cells of rLdPxn1 and rLdPxn2 immunized mice. Mice were immunized s.c. three times at three-week intervals with rLdPxn1 or rLdPxn2 with or without CpG ODN or GLA-SE. Four weeks after the last immunization, spleen cells were prepared and in vitro stimulated with the respective antigen (2 and 10 μg/mL) or ConA (5 μg/mL). The release of IFN-γ (ng/mL), IL-10 (ng/mL), and IL-4 (pg/mL) in immunized mice was measured in supernatants after 72 hr of in vitro stimulation at 37°C. Results are presented as the amount of IFN-γ (ng/mL) and IL-10 (ng/mL) for rLdPxn1 (a) and rLdPxn2 (b) or the amount of IL-4 (pg/mL) of rLdPxn1 and rLdPxn2 (c).
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Related In: Results  -  Collection


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fig5: Cytokine responses in spleen cells of rLdPxn1 and rLdPxn2 immunized mice. Mice were immunized s.c. three times at three-week intervals with rLdPxn1 or rLdPxn2 with or without CpG ODN or GLA-SE. Four weeks after the last immunization, spleen cells were prepared and in vitro stimulated with the respective antigen (2 and 10 μg/mL) or ConA (5 μg/mL). The release of IFN-γ (ng/mL), IL-10 (ng/mL), and IL-4 (pg/mL) in immunized mice was measured in supernatants after 72 hr of in vitro stimulation at 37°C. Results are presented as the amount of IFN-γ (ng/mL) and IL-10 (ng/mL) for rLdPxn1 (a) and rLdPxn2 (b) or the amount of IL-4 (pg/mL) of rLdPxn1 and rLdPxn2 (c).

Mentions: Production of IFN-γ, IL-10, and IL-4 by spleen cells of immunized mice is depicted in Figure 5.


Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice.

Daifalla NS, Bayih AG, Gedamu L - J Immunol Res (2015)

Cytokine responses in spleen cells of rLdPxn1 and rLdPxn2 immunized mice. Mice were immunized s.c. three times at three-week intervals with rLdPxn1 or rLdPxn2 with or without CpG ODN or GLA-SE. Four weeks after the last immunization, spleen cells were prepared and in vitro stimulated with the respective antigen (2 and 10 μg/mL) or ConA (5 μg/mL). The release of IFN-γ (ng/mL), IL-10 (ng/mL), and IL-4 (pg/mL) in immunized mice was measured in supernatants after 72 hr of in vitro stimulation at 37°C. Results are presented as the amount of IFN-γ (ng/mL) and IL-10 (ng/mL) for rLdPxn1 (a) and rLdPxn2 (b) or the amount of IL-4 (pg/mL) of rLdPxn1 and rLdPxn2 (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562178&req=5

fig5: Cytokine responses in spleen cells of rLdPxn1 and rLdPxn2 immunized mice. Mice were immunized s.c. three times at three-week intervals with rLdPxn1 or rLdPxn2 with or without CpG ODN or GLA-SE. Four weeks after the last immunization, spleen cells were prepared and in vitro stimulated with the respective antigen (2 and 10 μg/mL) or ConA (5 μg/mL). The release of IFN-γ (ng/mL), IL-10 (ng/mL), and IL-4 (pg/mL) in immunized mice was measured in supernatants after 72 hr of in vitro stimulation at 37°C. Results are presented as the amount of IFN-γ (ng/mL) and IL-10 (ng/mL) for rLdPxn1 (a) and rLdPxn2 (b) or the amount of IL-4 (pg/mL) of rLdPxn1 and rLdPxn2 (c).
Mentions: Production of IFN-γ, IL-10, and IL-4 by spleen cells of immunized mice is depicted in Figure 5.

Bottom Line: We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response.Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype.TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Calgary, Room 374, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4 ; The Forsyth Institute, Cambridge, MA 02142, USA.

ABSTRACT
We assessed the immune response against recombinant proteins of two related, albeit functionally different, peroxidoxins from Leishmania donovani: peroxidoxin 1 (LdPxn1) and peroxidoxin 2 (LdPxn2) in BALB/c mice. We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response. Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype. Antigen-stimulated spleen cells from mice that were immunized with rLdPxn1 produced low level of IL-10 and IL-4 and no IFN-γ, whereas cells from mice immunized with rLdPxn2 secreted high level of IFN-γ, low IL-4, and no IL-10. Coadministration of CpG ODN or GLA-SE with rLdPxn1 skewed the immune response towards a Th 1 type as indicated by robust production of IgG2a isotype. Furthermore, the presence of TLR agonists together with rLdPxn1 antigen enhanced the production of IFN-γ and to a lesser extent of IL-10. TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

No MeSH data available.