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Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice.

Daifalla NS, Bayih AG, Gedamu L - J Immunol Res (2015)

Bottom Line: We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response.Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype.TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Calgary, Room 374, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4 ; The Forsyth Institute, Cambridge, MA 02142, USA.

ABSTRACT
We assessed the immune response against recombinant proteins of two related, albeit functionally different, peroxidoxins from Leishmania donovani: peroxidoxin 1 (LdPxn1) and peroxidoxin 2 (LdPxn2) in BALB/c mice. We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response. Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype. Antigen-stimulated spleen cells from mice that were immunized with rLdPxn1 produced low level of IL-10 and IL-4 and no IFN-γ, whereas cells from mice immunized with rLdPxn2 secreted high level of IFN-γ, low IL-4, and no IL-10. Coadministration of CpG ODN or GLA-SE with rLdPxn1 skewed the immune response towards a Th 1 type as indicated by robust production of IgG2a isotype. Furthermore, the presence of TLR agonists together with rLdPxn1 antigen enhanced the production of IFN-γ and to a lesser extent of IL-10. TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

No MeSH data available.


Related in: MedlinePlus

Anti-LdPxn1 and -LdPxn2 antibodies in immunized mice. Mice were immunized subcutaneously with recombinant LdPxn1 or LdPxn2 proteins with or without CpG ODN or GLA-SE. Mice were boosted twice in 3-week intervals. The levels of IgG1 and IgG2a isotypes were measured on sera collected at different time points using ELISA. Data are presented as the mean OD ± S.E.M. of IgG1 and IgG2a of sera from mice immunized with rLdPxn1 (a) and rLdPxn2 (b). The IgG2a/IgG1 ratios are shown in tables below each figure.
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fig3: Anti-LdPxn1 and -LdPxn2 antibodies in immunized mice. Mice were immunized subcutaneously with recombinant LdPxn1 or LdPxn2 proteins with or without CpG ODN or GLA-SE. Mice were boosted twice in 3-week intervals. The levels of IgG1 and IgG2a isotypes were measured on sera collected at different time points using ELISA. Data are presented as the mean OD ± S.E.M. of IgG1 and IgG2a of sera from mice immunized with rLdPxn1 (a) and rLdPxn2 (b). The IgG2a/IgG1 ratios are shown in tables below each figure.

Mentions: As shown in Figure 3(a), immunization of mice with rLdPxn1 by itself stimulated a high level of IgG1 isotype and barely detectable amount of IgG2a at 3 weeks after the first immunization. The amount of specific IgG2a stimulated in this group increased upon booster immunization; however it remains significantly lower than the amount of IgG1 (P < 0.05). Concomitant injection of CpG ODN or GLA-SE with rLdPxn1 triggered a high level of IgG1 and more importantly a high level of IgG2a as well (Figure 3(a)). Similar to immunization with rLdPxn1 alone, the production of anti-rLdPxn1 antibodies in mice immunized with rLdPxn1 plus adjuvants was augmented by booster immunization (Figure 3(a)). The augmentation effect of booster injections together with the presence of TLR agonists in the immunization protocol resulted in the induction of IgG2a level as high as IgG1 four weeks after the last boost.


Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice.

Daifalla NS, Bayih AG, Gedamu L - J Immunol Res (2015)

Anti-LdPxn1 and -LdPxn2 antibodies in immunized mice. Mice were immunized subcutaneously with recombinant LdPxn1 or LdPxn2 proteins with or without CpG ODN or GLA-SE. Mice were boosted twice in 3-week intervals. The levels of IgG1 and IgG2a isotypes were measured on sera collected at different time points using ELISA. Data are presented as the mean OD ± S.E.M. of IgG1 and IgG2a of sera from mice immunized with rLdPxn1 (a) and rLdPxn2 (b). The IgG2a/IgG1 ratios are shown in tables below each figure.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Anti-LdPxn1 and -LdPxn2 antibodies in immunized mice. Mice were immunized subcutaneously with recombinant LdPxn1 or LdPxn2 proteins with or without CpG ODN or GLA-SE. Mice were boosted twice in 3-week intervals. The levels of IgG1 and IgG2a isotypes were measured on sera collected at different time points using ELISA. Data are presented as the mean OD ± S.E.M. of IgG1 and IgG2a of sera from mice immunized with rLdPxn1 (a) and rLdPxn2 (b). The IgG2a/IgG1 ratios are shown in tables below each figure.
Mentions: As shown in Figure 3(a), immunization of mice with rLdPxn1 by itself stimulated a high level of IgG1 isotype and barely detectable amount of IgG2a at 3 weeks after the first immunization. The amount of specific IgG2a stimulated in this group increased upon booster immunization; however it remains significantly lower than the amount of IgG1 (P < 0.05). Concomitant injection of CpG ODN or GLA-SE with rLdPxn1 triggered a high level of IgG1 and more importantly a high level of IgG2a as well (Figure 3(a)). Similar to immunization with rLdPxn1 alone, the production of anti-rLdPxn1 antibodies in mice immunized with rLdPxn1 plus adjuvants was augmented by booster immunization (Figure 3(a)). The augmentation effect of booster injections together with the presence of TLR agonists in the immunization protocol resulted in the induction of IgG2a level as high as IgG1 four weeks after the last boost.

Bottom Line: We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response.Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype.TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Calgary, Room 374, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4 ; The Forsyth Institute, Cambridge, MA 02142, USA.

ABSTRACT
We assessed the immune response against recombinant proteins of two related, albeit functionally different, peroxidoxins from Leishmania donovani: peroxidoxin 1 (LdPxn1) and peroxidoxin 2 (LdPxn2) in BALB/c mice. We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response. Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype. Antigen-stimulated spleen cells from mice that were immunized with rLdPxn1 produced low level of IL-10 and IL-4 and no IFN-γ, whereas cells from mice immunized with rLdPxn2 secreted high level of IFN-γ, low IL-4, and no IL-10. Coadministration of CpG ODN or GLA-SE with rLdPxn1 skewed the immune response towards a Th 1 type as indicated by robust production of IgG2a isotype. Furthermore, the presence of TLR agonists together with rLdPxn1 antigen enhanced the production of IFN-γ and to a lesser extent of IL-10. TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.

No MeSH data available.


Related in: MedlinePlus