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Comparison of Alcian Blue, Trypan Blue, and Toluidine Blue for Visualization of the Primo Vascular System Floating in Lymph Ducts.

Kim DU, Han JW, Jung SJ, Lee SH, Cha R, Chang BS, Soh KS - Evid Based Complement Alternat Med (2015)

Bottom Line: We found that Trypan blue and toluidine blue did not visualize the PVS.Trypan blue was cleared by the natural washing.These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

View Article: PubMed Central - PubMed

Affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea ; College of Physical Education, The University of Suwon, Hwaseong 445-743, Republic of Korea.

ABSTRACT
The primo vascular system (PVS), floating in lymph ducts, was too transparent to be observed by using a stereomicroscope. It was only detectable with the aid of staining dyes, for instance, Alcian blue, which was injected into the lymph nodes. Some dyes were absorbed preferentially by the PVS than the lymph wall. It remains a standing problem to know what dyes are absorbed better by the PVS than the lymph walls. Such information would be useful to unravel the biochemical properties of the PVS that are badly in need for obtaining large amount of PVS specimens. In the current work we tried two other familiar dyes which were used in PVS research before. We found that Trypan blue and toluidine blue did not visualize the PVS. Trypan blue was cleared by the natural washing. Toluidine blue did not stain the PVS, but it did leave stained spots in the lymph wall and its surrounding tissues, and it leaked out of the lymph wall to stain surrounding connective tissues. These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

No MeSH data available.


Related in: MedlinePlus

Comparison of lymph ducts into which Alcian blue and toluidine blue had been injected. (a) Two branches of lymph ducts (one of them indicated with double arrows) and their L-PVS stained with Alcian blue. (b) Toluidine blue left stained spots in the lymph wall (arrow heads). It also leaked from the wall and stained the surrounding tissues. However, the L-PVS was not stained. (c) A thin blood vessel was deeply stained by using toluidine blue. How it flowed into the blood vessel is not known.
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fig4: Comparison of lymph ducts into which Alcian blue and toluidine blue had been injected. (a) Two branches of lymph ducts (one of them indicated with double arrows) and their L-PVS stained with Alcian blue. (b) Toluidine blue left stained spots in the lymph wall (arrow heads). It also leaked from the wall and stained the surrounding tissues. However, the L-PVS was not stained. (c) A thin blood vessel was deeply stained by using toluidine blue. How it flowed into the blood vessel is not known.

Mentions: Figure 4(a) shows another Alcian-blue-stained primo vessel in comparison with the toluidine blue injection case (Figure 4(b)) in which no primo vessel was observed, but in which the lymph duct and the surrounding tissue were weakly stained. In addition, strongly stained spots were sporadically distributed. The dye leaked from the lymph duct. Furthermore, some small blood vessels were strongly stained, and apparently the dye flowed in the blood vessel (Figure 4(c)). This leaking from the lymph duct and flowing in blood vessels of toluidine blue were observed for the first time in this work.


Comparison of Alcian Blue, Trypan Blue, and Toluidine Blue for Visualization of the Primo Vascular System Floating in Lymph Ducts.

Kim DU, Han JW, Jung SJ, Lee SH, Cha R, Chang BS, Soh KS - Evid Based Complement Alternat Med (2015)

Comparison of lymph ducts into which Alcian blue and toluidine blue had been injected. (a) Two branches of lymph ducts (one of them indicated with double arrows) and their L-PVS stained with Alcian blue. (b) Toluidine blue left stained spots in the lymph wall (arrow heads). It also leaked from the wall and stained the surrounding tissues. However, the L-PVS was not stained. (c) A thin blood vessel was deeply stained by using toluidine blue. How it flowed into the blood vessel is not known.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562098&req=5

fig4: Comparison of lymph ducts into which Alcian blue and toluidine blue had been injected. (a) Two branches of lymph ducts (one of them indicated with double arrows) and their L-PVS stained with Alcian blue. (b) Toluidine blue left stained spots in the lymph wall (arrow heads). It also leaked from the wall and stained the surrounding tissues. However, the L-PVS was not stained. (c) A thin blood vessel was deeply stained by using toluidine blue. How it flowed into the blood vessel is not known.
Mentions: Figure 4(a) shows another Alcian-blue-stained primo vessel in comparison with the toluidine blue injection case (Figure 4(b)) in which no primo vessel was observed, but in which the lymph duct and the surrounding tissue were weakly stained. In addition, strongly stained spots were sporadically distributed. The dye leaked from the lymph duct. Furthermore, some small blood vessels were strongly stained, and apparently the dye flowed in the blood vessel (Figure 4(c)). This leaking from the lymph duct and flowing in blood vessels of toluidine blue were observed for the first time in this work.

Bottom Line: We found that Trypan blue and toluidine blue did not visualize the PVS.Trypan blue was cleared by the natural washing.These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

View Article: PubMed Central - PubMed

Affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea ; College of Physical Education, The University of Suwon, Hwaseong 445-743, Republic of Korea.

ABSTRACT
The primo vascular system (PVS), floating in lymph ducts, was too transparent to be observed by using a stereomicroscope. It was only detectable with the aid of staining dyes, for instance, Alcian blue, which was injected into the lymph nodes. Some dyes were absorbed preferentially by the PVS than the lymph wall. It remains a standing problem to know what dyes are absorbed better by the PVS than the lymph walls. Such information would be useful to unravel the biochemical properties of the PVS that are badly in need for obtaining large amount of PVS specimens. In the current work we tried two other familiar dyes which were used in PVS research before. We found that Trypan blue and toluidine blue did not visualize the PVS. Trypan blue was cleared by the natural washing. Toluidine blue did not stain the PVS, but it did leave stained spots in the lymph wall and its surrounding tissues, and it leaked out of the lymph wall to stain surrounding connective tissues. These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

No MeSH data available.


Related in: MedlinePlus