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Comparison of Alcian Blue, Trypan Blue, and Toluidine Blue for Visualization of the Primo Vascular System Floating in Lymph Ducts.

Kim DU, Han JW, Jung SJ, Lee SH, Cha R, Chang BS, Soh KS - Evid Based Complement Alternat Med (2015)

Bottom Line: We found that Trypan blue and toluidine blue did not visualize the PVS.Trypan blue was cleared by the natural washing.These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

View Article: PubMed Central - PubMed

Affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea ; College of Physical Education, The University of Suwon, Hwaseong 445-743, Republic of Korea.

ABSTRACT
The primo vascular system (PVS), floating in lymph ducts, was too transparent to be observed by using a stereomicroscope. It was only detectable with the aid of staining dyes, for instance, Alcian blue, which was injected into the lymph nodes. Some dyes were absorbed preferentially by the PVS than the lymph wall. It remains a standing problem to know what dyes are absorbed better by the PVS than the lymph walls. Such information would be useful to unravel the biochemical properties of the PVS that are badly in need for obtaining large amount of PVS specimens. In the current work we tried two other familiar dyes which were used in PVS research before. We found that Trypan blue and toluidine blue did not visualize the PVS. Trypan blue was cleared by the natural washing. Toluidine blue did not stain the PVS, but it did leave stained spots in the lymph wall and its surrounding tissues, and it leaked out of the lymph wall to stain surrounding connective tissues. These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

No MeSH data available.


Related in: MedlinePlus

Comparison of stereomicroscopic images of the lymph ducts into which Alcian blue and Trypan blue had been injected. (a) A primo vessel emerged in a lymph duct (double arrows) after a two-hour washing of the injected Alcian blue. This image corresponds to Figure 1(e). (b) The lymph duct after a two-hour washing of the injected Trypan blue became clear again without any hint of the presence of the primo vessel. This showed that the L-PVS was selectively stained by the Alcian blue, not by the Trypan blue. (c) Contrast-enhanced microscope image of a branched lymph duct (double arrows) that was extracted and put on a slide. Connective tissues wrapped the lymph duct. The stained L-PVS also branched.
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fig3: Comparison of stereomicroscopic images of the lymph ducts into which Alcian blue and Trypan blue had been injected. (a) A primo vessel emerged in a lymph duct (double arrows) after a two-hour washing of the injected Alcian blue. This image corresponds to Figure 1(e). (b) The lymph duct after a two-hour washing of the injected Trypan blue became clear again without any hint of the presence of the primo vessel. This showed that the L-PVS was selectively stained by the Alcian blue, not by the Trypan blue. (c) Contrast-enhanced microscope image of a branched lymph duct (double arrows) that was extracted and put on a slide. Connective tissues wrapped the lymph duct. The stained L-PVS also branched.

Mentions: Figure 3(a) shows the magnified images of the primo vessel inside the lymph duct. Figure 3(b) shows the transparent lymph duct after washing of the preinjected Trypan blue. The L-PVS was not stained at all, so it was not observable. Figure 3(c), an enhanced contrast microscope image of the specimen on a slide glass, shows an extracted lymph duct with the Alcian-blue-stained primo vessel in it. A small part of the primo vessel was drawn out on the left upper part of the lymph duct. This demonstrates the existence of an independent threadlike object inside the lymph duct. Also, the primo vessel is seen to branch where the lymph duct branched.


Comparison of Alcian Blue, Trypan Blue, and Toluidine Blue for Visualization of the Primo Vascular System Floating in Lymph Ducts.

Kim DU, Han JW, Jung SJ, Lee SH, Cha R, Chang BS, Soh KS - Evid Based Complement Alternat Med (2015)

Comparison of stereomicroscopic images of the lymph ducts into which Alcian blue and Trypan blue had been injected. (a) A primo vessel emerged in a lymph duct (double arrows) after a two-hour washing of the injected Alcian blue. This image corresponds to Figure 1(e). (b) The lymph duct after a two-hour washing of the injected Trypan blue became clear again without any hint of the presence of the primo vessel. This showed that the L-PVS was selectively stained by the Alcian blue, not by the Trypan blue. (c) Contrast-enhanced microscope image of a branched lymph duct (double arrows) that was extracted and put on a slide. Connective tissues wrapped the lymph duct. The stained L-PVS also branched.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562098&req=5

fig3: Comparison of stereomicroscopic images of the lymph ducts into which Alcian blue and Trypan blue had been injected. (a) A primo vessel emerged in a lymph duct (double arrows) after a two-hour washing of the injected Alcian blue. This image corresponds to Figure 1(e). (b) The lymph duct after a two-hour washing of the injected Trypan blue became clear again without any hint of the presence of the primo vessel. This showed that the L-PVS was selectively stained by the Alcian blue, not by the Trypan blue. (c) Contrast-enhanced microscope image of a branched lymph duct (double arrows) that was extracted and put on a slide. Connective tissues wrapped the lymph duct. The stained L-PVS also branched.
Mentions: Figure 3(a) shows the magnified images of the primo vessel inside the lymph duct. Figure 3(b) shows the transparent lymph duct after washing of the preinjected Trypan blue. The L-PVS was not stained at all, so it was not observable. Figure 3(c), an enhanced contrast microscope image of the specimen on a slide glass, shows an extracted lymph duct with the Alcian-blue-stained primo vessel in it. A small part of the primo vessel was drawn out on the left upper part of the lymph duct. This demonstrates the existence of an independent threadlike object inside the lymph duct. Also, the primo vessel is seen to branch where the lymph duct branched.

Bottom Line: We found that Trypan blue and toluidine blue did not visualize the PVS.Trypan blue was cleared by the natural washing.These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

View Article: PubMed Central - PubMed

Affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea ; College of Physical Education, The University of Suwon, Hwaseong 445-743, Republic of Korea.

ABSTRACT
The primo vascular system (PVS), floating in lymph ducts, was too transparent to be observed by using a stereomicroscope. It was only detectable with the aid of staining dyes, for instance, Alcian blue, which was injected into the lymph nodes. Some dyes were absorbed preferentially by the PVS than the lymph wall. It remains a standing problem to know what dyes are absorbed better by the PVS than the lymph walls. Such information would be useful to unravel the biochemical properties of the PVS that are badly in need for obtaining large amount of PVS specimens. In the current work we tried two other familiar dyes which were used in PVS research before. We found that Trypan blue and toluidine blue did not visualize the PVS. Trypan blue was cleared by the natural washing. Toluidine blue did not stain the PVS, but it did leave stained spots in the lymph wall and its surrounding tissues, and it leaked out of the lymph wall to stain surrounding connective tissues. These completely different behaviors of the three dyes were found for the first time in the current work and provide valuable information to elucidate the mechanism through which some special dyes stained the PVS preferentially compared to the lymphatic wall.

No MeSH data available.


Related in: MedlinePlus