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Primo-Vascular System as Presented by Bong Han Kim.

Vodyanoy V, Pustovyy O, Globa L, Sorokulova I - Evid Based Complement Alternat Med (2015)

Bottom Line: However, he did not disclose in detail his methods.Consequently, his results are relatively obscure from the vantage point of contemporary scientists.Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn, AL 36849, USA ; School of Kinesiology, Auburn University, Auburn, AL 36849, USA ; Edward Via College of Osteopathic Medicine, Auburn, AL 36849, USA.

ABSTRACT
In the 1960s Bong Han Kim discovered and characterized a new vascular system. He was able to differentiate it clearly from vascular blood and lymph systems by the use of a variety of methods, which were available to him in the mid-20th century. He gave detailed characterization of the system and created comprehensive diagrams and photographs in his publications. He demonstrated that this system is composed of nodes and vessels, and it was responsible for tissue regeneration. However, he did not disclose in detail his methods. Consequently, his results are relatively obscure from the vantage point of contemporary scientists. The stains that Kim used had been perfected and had been in use for more than 100 years. Therefore, the names of the stains were directed to the explicit protocols for the usage with the particular cells or molecules. Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method. In this present work, we have been able to disclose staining methods used by Kim.

No MeSH data available.


Related in: MedlinePlus

TEM micrographs of p-microcells, very small embryonic-like (VSEL), and hematopoietic stem cells. (a) External primo-node. Star: p-microcells. (b) P-microcells. BSS: p-microcell nucleosome, BSP: p-microcell nucleoplasm, and BSM: p-microcell membrane [7]. (c) TEM of very small embryonic-like (VSEL) cells and hematopoietic stem cells. (A) Small embryonic-like (VSEL) cells are small and measure 2–4 μm in diameter. They possess a relatively large nucleus surrounded by a narrow rim of cytoplasm. The narrow rim of cytoplasm possesses a few mitochondria, scattered ribosomes, small profiles of endoplasmic reticulum, and a few vesicles. The nucleus is contained within a nuclear envelope with nuclear pores. Chromatin is loosely packed and consists of euchromatin. (B) In contrast hematopoietic stem cells display heterogeneous morphology and are larger. They measure on average 8–10 μm in diameter and possess scattered chromatin and prominent nucleoli (reprinted by permission from Macmillan Publishers Ltd. leukemia [36], copyright, 2006).
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fig7: TEM micrographs of p-microcells, very small embryonic-like (VSEL), and hematopoietic stem cells. (a) External primo-node. Star: p-microcells. (b) P-microcells. BSS: p-microcell nucleosome, BSP: p-microcell nucleoplasm, and BSM: p-microcell membrane [7]. (c) TEM of very small embryonic-like (VSEL) cells and hematopoietic stem cells. (A) Small embryonic-like (VSEL) cells are small and measure 2–4 μm in diameter. They possess a relatively large nucleus surrounded by a narrow rim of cytoplasm. The narrow rim of cytoplasm possesses a few mitochondria, scattered ribosomes, small profiles of endoplasmic reticulum, and a few vesicles. The nucleus is contained within a nuclear envelope with nuclear pores. Chromatin is loosely packed and consists of euchromatin. (B) In contrast hematopoietic stem cells display heterogeneous morphology and are larger. They measure on average 8–10 μm in diameter and possess scattered chromatin and prominent nucleoli (reprinted by permission from Macmillan Publishers Ltd. leukemia [36], copyright, 2006).

Mentions: Kim studied the structure of p-microcell, the electron microscope. Figure 7(a) shows p-microcells inside a small external primo-node. Kim thoroughly described p-microcells found within the node. The p-microcells vary in shape. Some are round and others irregular, and their membranes are smooth. In the cytoplasm of some of the round cells, endoplasmic reticulum is well developed; it is organized around the nucleus and mitochondria. In the cytoplasm of the cells, one nucleus or two nuclei can be found in the middle or periphery of the cell. These nuclei have thin membranes and the abundant chromatin, and the nucleoli are located down in the middle of the nuclei. The nuclear membrane occasionally is wrinkled. The boundary of the cell is smooth or occasionally has processes. In the cytoplasm, there are numerous vacuolar structures. The cytoplasm of some cells includes vesicular endoplasmic reticulum and many small round granules of high electron density. However, such granules cannot be noticed in the vacuoles. Mitochondria of an oblong shape are normally found in a part of the cytoplasm. Adjoining cells come close to each other or are linked to one another via small cytoplasmic processes [7].


Primo-Vascular System as Presented by Bong Han Kim.

Vodyanoy V, Pustovyy O, Globa L, Sorokulova I - Evid Based Complement Alternat Med (2015)

TEM micrographs of p-microcells, very small embryonic-like (VSEL), and hematopoietic stem cells. (a) External primo-node. Star: p-microcells. (b) P-microcells. BSS: p-microcell nucleosome, BSP: p-microcell nucleoplasm, and BSM: p-microcell membrane [7]. (c) TEM of very small embryonic-like (VSEL) cells and hematopoietic stem cells. (A) Small embryonic-like (VSEL) cells are small and measure 2–4 μm in diameter. They possess a relatively large nucleus surrounded by a narrow rim of cytoplasm. The narrow rim of cytoplasm possesses a few mitochondria, scattered ribosomes, small profiles of endoplasmic reticulum, and a few vesicles. The nucleus is contained within a nuclear envelope with nuclear pores. Chromatin is loosely packed and consists of euchromatin. (B) In contrast hematopoietic stem cells display heterogeneous morphology and are larger. They measure on average 8–10 μm in diameter and possess scattered chromatin and prominent nucleoli (reprinted by permission from Macmillan Publishers Ltd. leukemia [36], copyright, 2006).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562093&req=5

fig7: TEM micrographs of p-microcells, very small embryonic-like (VSEL), and hematopoietic stem cells. (a) External primo-node. Star: p-microcells. (b) P-microcells. BSS: p-microcell nucleosome, BSP: p-microcell nucleoplasm, and BSM: p-microcell membrane [7]. (c) TEM of very small embryonic-like (VSEL) cells and hematopoietic stem cells. (A) Small embryonic-like (VSEL) cells are small and measure 2–4 μm in diameter. They possess a relatively large nucleus surrounded by a narrow rim of cytoplasm. The narrow rim of cytoplasm possesses a few mitochondria, scattered ribosomes, small profiles of endoplasmic reticulum, and a few vesicles. The nucleus is contained within a nuclear envelope with nuclear pores. Chromatin is loosely packed and consists of euchromatin. (B) In contrast hematopoietic stem cells display heterogeneous morphology and are larger. They measure on average 8–10 μm in diameter and possess scattered chromatin and prominent nucleoli (reprinted by permission from Macmillan Publishers Ltd. leukemia [36], copyright, 2006).
Mentions: Kim studied the structure of p-microcell, the electron microscope. Figure 7(a) shows p-microcells inside a small external primo-node. Kim thoroughly described p-microcells found within the node. The p-microcells vary in shape. Some are round and others irregular, and their membranes are smooth. In the cytoplasm of some of the round cells, endoplasmic reticulum is well developed; it is organized around the nucleus and mitochondria. In the cytoplasm of the cells, one nucleus or two nuclei can be found in the middle or periphery of the cell. These nuclei have thin membranes and the abundant chromatin, and the nucleoli are located down in the middle of the nuclei. The nuclear membrane occasionally is wrinkled. The boundary of the cell is smooth or occasionally has processes. In the cytoplasm, there are numerous vacuolar structures. The cytoplasm of some cells includes vesicular endoplasmic reticulum and many small round granules of high electron density. However, such granules cannot be noticed in the vacuoles. Mitochondria of an oblong shape are normally found in a part of the cytoplasm. Adjoining cells come close to each other or are linked to one another via small cytoplasmic processes [7].

Bottom Line: However, he did not disclose in detail his methods.Consequently, his results are relatively obscure from the vantage point of contemporary scientists.Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn, AL 36849, USA ; School of Kinesiology, Auburn University, Auburn, AL 36849, USA ; Edward Via College of Osteopathic Medicine, Auburn, AL 36849, USA.

ABSTRACT
In the 1960s Bong Han Kim discovered and characterized a new vascular system. He was able to differentiate it clearly from vascular blood and lymph systems by the use of a variety of methods, which were available to him in the mid-20th century. He gave detailed characterization of the system and created comprehensive diagrams and photographs in his publications. He demonstrated that this system is composed of nodes and vessels, and it was responsible for tissue regeneration. However, he did not disclose in detail his methods. Consequently, his results are relatively obscure from the vantage point of contemporary scientists. The stains that Kim used had been perfected and had been in use for more than 100 years. Therefore, the names of the stains were directed to the explicit protocols for the usage with the particular cells or molecules. Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method. In this present work, we have been able to disclose staining methods used by Kim.

No MeSH data available.


Related in: MedlinePlus