Limits...
Primo-Vascular System as Presented by Bong Han Kim.

Vodyanoy V, Pustovyy O, Globa L, Sorokulova I - Evid Based Complement Alternat Med (2015)

Bottom Line: However, he did not disclose in detail his methods.Consequently, his results are relatively obscure from the vantage point of contemporary scientists.Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn, AL 36849, USA ; School of Kinesiology, Auburn University, Auburn, AL 36849, USA ; Edward Via College of Osteopathic Medicine, Auburn, AL 36849, USA.

ABSTRACT
In the 1960s Bong Han Kim discovered and characterized a new vascular system. He was able to differentiate it clearly from vascular blood and lymph systems by the use of a variety of methods, which were available to him in the mid-20th century. He gave detailed characterization of the system and created comprehensive diagrams and photographs in his publications. He demonstrated that this system is composed of nodes and vessels, and it was responsible for tissue regeneration. However, he did not disclose in detail his methods. Consequently, his results are relatively obscure from the vantage point of contemporary scientists. The stains that Kim used had been perfected and had been in use for more than 100 years. Therefore, the names of the stains were directed to the explicit protocols for the usage with the particular cells or molecules. Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method. In this present work, we have been able to disclose staining methods used by Kim.

No MeSH data available.


Related in: MedlinePlus

Superficial primo-node. (a) Feulgen stain. Sinus of the superficial primo-node (arrow) (×160). (b) Hillarp-Hokfelt stain. 1: chromaffin cell, 2: blood vessel. (c) Sevki stain; 1: chromaffin cell; 2: blood vessel [7].
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4562093&req=5

fig3: Superficial primo-node. (a) Feulgen stain. Sinus of the superficial primo-node (arrow) (×160). (b) Hillarp-Hokfelt stain. 1: chromaffin cell, 2: blood vessel. (c) Sevki stain; 1: chromaffin cell; 2: blood vessel [7].

Mentions: Kim used Feulgen, Unna-Pappenheim, Brachet, acridine orange, and hematoxylin-eosin stains to characterize nucleic acid distribution in PVS (Table 1). The Feulgen stain was used to visualize cell nuclei, basophile granules, and other structures containing DNA inside p-subvessels by virtue of its capacity to penetrate the cellular membrane. He also imaged basophile particles and p-microcells in the sinuses of primo-nodes (Figure 3(a)). As a result of acid hydrolysis of DNAs [38], it stains them red as showed in many images of endothelial cell nuclei in walls of p-subvessels. The elongated, rod-like nuclei of p-subvessel endothelial cells work as a unique marker of PVS. In Kim's studies, the DNA of p-microcell nucleosome was stained by Feulgen reaction while DNA in p-microcell nucleoplasm was revealed by the Brachet stain [7].


Primo-Vascular System as Presented by Bong Han Kim.

Vodyanoy V, Pustovyy O, Globa L, Sorokulova I - Evid Based Complement Alternat Med (2015)

Superficial primo-node. (a) Feulgen stain. Sinus of the superficial primo-node (arrow) (×160). (b) Hillarp-Hokfelt stain. 1: chromaffin cell, 2: blood vessel. (c) Sevki stain; 1: chromaffin cell; 2: blood vessel [7].
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562093&req=5

fig3: Superficial primo-node. (a) Feulgen stain. Sinus of the superficial primo-node (arrow) (×160). (b) Hillarp-Hokfelt stain. 1: chromaffin cell, 2: blood vessel. (c) Sevki stain; 1: chromaffin cell; 2: blood vessel [7].
Mentions: Kim used Feulgen, Unna-Pappenheim, Brachet, acridine orange, and hematoxylin-eosin stains to characterize nucleic acid distribution in PVS (Table 1). The Feulgen stain was used to visualize cell nuclei, basophile granules, and other structures containing DNA inside p-subvessels by virtue of its capacity to penetrate the cellular membrane. He also imaged basophile particles and p-microcells in the sinuses of primo-nodes (Figure 3(a)). As a result of acid hydrolysis of DNAs [38], it stains them red as showed in many images of endothelial cell nuclei in walls of p-subvessels. The elongated, rod-like nuclei of p-subvessel endothelial cells work as a unique marker of PVS. In Kim's studies, the DNA of p-microcell nucleosome was stained by Feulgen reaction while DNA in p-microcell nucleoplasm was revealed by the Brachet stain [7].

Bottom Line: However, he did not disclose in detail his methods.Consequently, his results are relatively obscure from the vantage point of contemporary scientists.Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn, AL 36849, USA ; School of Kinesiology, Auburn University, Auburn, AL 36849, USA ; Edward Via College of Osteopathic Medicine, Auburn, AL 36849, USA.

ABSTRACT
In the 1960s Bong Han Kim discovered and characterized a new vascular system. He was able to differentiate it clearly from vascular blood and lymph systems by the use of a variety of methods, which were available to him in the mid-20th century. He gave detailed characterization of the system and created comprehensive diagrams and photographs in his publications. He demonstrated that this system is composed of nodes and vessels, and it was responsible for tissue regeneration. However, he did not disclose in detail his methods. Consequently, his results are relatively obscure from the vantage point of contemporary scientists. The stains that Kim used had been perfected and had been in use for more than 100 years. Therefore, the names of the stains were directed to the explicit protocols for the usage with the particular cells or molecules. Traditionally, it was not normally necessary to describe the method used unless it is significantly deviated from the original method. In this present work, we have been able to disclose staining methods used by Kim.

No MeSH data available.


Related in: MedlinePlus