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Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis.

Ping A, Zhendong S, Rongmei Q, Jingxing D, Wei C, Zhongyin Z, Hesheng L, Soh KS - Evid Based Complement Alternat Med (2015)

Bottom Line: We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them.We deduced that primo vessels may be precursors of blood vessels.These results possibly provided a thoroughly new theoretic development in cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430070, China.

ABSTRACT
Gastric cancer is the fourth commonest cancer in the world and the second leading cause of cancer-related death. Investigation of gastric cancer metastasis is one of the hottest and major focuses in cancer research. Growing evidence manifested that primo vascular system (PVS) is a new kind of circulatory system beyond vascular and lymphatic system. Previous researches revealed that PVS is a specific tissue between endothelium and mesenchyme and is involved in cancer, especially in tumor metastasis and regeneration. In current study, we investigated the role of primo vessels in gastric cancer metastasis and its possible relationship to vascular vessels formation. Our results indicated that primo vessels were involved in gastric cancer metastasis. We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them. We deduced that primo vessels may be precursors of blood vessels. These results possibly provided a thoroughly new theoretic development in cancer metastasis.

No MeSH data available.


Related in: MedlinePlus

Primo vessels were involved in gastric cancer metastasis. (a) A free primo vessel (black arrow) with a thread-like and semitransparent appearance between tumors. (b) Under the LZ12 microscope, a tiny metastatic tumor (black arrow) was observed in the primo vessel. (c) Magnification of (b); more microtumors or gastric cancer cells (yellow arrows) were detected. (d) HE staining for the metastatic tumor mediated by primo vessels. (e) Magnification of framed area of (d) (Bar: 100 μm). (f) Transversal section of the primo vessel embedded was stained by H&E (Bar: 50 μm). No vascular vessels or lymphatic vessels were observed in either metastatic tumors or primo vessels. (g) Primo vessels involved in gastric cancer metastasis showed rod shape nuclei in a linear arrangement (white arrows) and positively expressed phalloidin.
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fig3: Primo vessels were involved in gastric cancer metastasis. (a) A free primo vessel (black arrow) with a thread-like and semitransparent appearance between tumors. (b) Under the LZ12 microscope, a tiny metastatic tumor (black arrow) was observed in the primo vessel. (c) Magnification of (b); more microtumors or gastric cancer cells (yellow arrows) were detected. (d) HE staining for the metastatic tumor mediated by primo vessels. (e) Magnification of framed area of (d) (Bar: 100 μm). (f) Transversal section of the primo vessel embedded was stained by H&E (Bar: 50 μm). No vascular vessels or lymphatic vessels were observed in either metastatic tumors or primo vessels. (g) Primo vessels involved in gastric cancer metastasis showed rod shape nuclei in a linear arrangement (white arrows) and positively expressed phalloidin.

Mentions: Next, we investigated the role of primo vascular system in gastric cancer metastasis. We discovered that in orthotopic implantation mice, between the orthotopic tumors and the metastatic tumors, there are free thread-like and semitransparent structures that possess the basic features of primo vessels (Figure 3(a)). Under the LZ12 microscope, besides the fact that metastatic tumors with GFP fluorescence were distinctly detected, tiny tumors in primo vessels were clearly observed (Figure 3(b)). Magnified images revealed multiple microtumors in primo vessels (Figure 3(c)). More importantly, in H&E staining, no vascular vessels or lymphatic vessels were observed in metastatic tumors (Figures 3(d) and 3(e)) and primo vessels (Figure 3(f)). Phalloidin and DAPI staining revealed that these primo vessels expressed a linear arrangement of rod shape nuclei and positive phalloidin expression which conformed with the characters of primo vessels (Figure 3(g)). Accordant with our previous studies, the structure of primo vessels was obviously distinct from that of vascular vessels and lymphatic vessels.


Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis.

Ping A, Zhendong S, Rongmei Q, Jingxing D, Wei C, Zhongyin Z, Hesheng L, Soh KS - Evid Based Complement Alternat Med (2015)

Primo vessels were involved in gastric cancer metastasis. (a) A free primo vessel (black arrow) with a thread-like and semitransparent appearance between tumors. (b) Under the LZ12 microscope, a tiny metastatic tumor (black arrow) was observed in the primo vessel. (c) Magnification of (b); more microtumors or gastric cancer cells (yellow arrows) were detected. (d) HE staining for the metastatic tumor mediated by primo vessels. (e) Magnification of framed area of (d) (Bar: 100 μm). (f) Transversal section of the primo vessel embedded was stained by H&E (Bar: 50 μm). No vascular vessels or lymphatic vessels were observed in either metastatic tumors or primo vessels. (g) Primo vessels involved in gastric cancer metastasis showed rod shape nuclei in a linear arrangement (white arrows) and positively expressed phalloidin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562087&req=5

fig3: Primo vessels were involved in gastric cancer metastasis. (a) A free primo vessel (black arrow) with a thread-like and semitransparent appearance between tumors. (b) Under the LZ12 microscope, a tiny metastatic tumor (black arrow) was observed in the primo vessel. (c) Magnification of (b); more microtumors or gastric cancer cells (yellow arrows) were detected. (d) HE staining for the metastatic tumor mediated by primo vessels. (e) Magnification of framed area of (d) (Bar: 100 μm). (f) Transversal section of the primo vessel embedded was stained by H&E (Bar: 50 μm). No vascular vessels or lymphatic vessels were observed in either metastatic tumors or primo vessels. (g) Primo vessels involved in gastric cancer metastasis showed rod shape nuclei in a linear arrangement (white arrows) and positively expressed phalloidin.
Mentions: Next, we investigated the role of primo vascular system in gastric cancer metastasis. We discovered that in orthotopic implantation mice, between the orthotopic tumors and the metastatic tumors, there are free thread-like and semitransparent structures that possess the basic features of primo vessels (Figure 3(a)). Under the LZ12 microscope, besides the fact that metastatic tumors with GFP fluorescence were distinctly detected, tiny tumors in primo vessels were clearly observed (Figure 3(b)). Magnified images revealed multiple microtumors in primo vessels (Figure 3(c)). More importantly, in H&E staining, no vascular vessels or lymphatic vessels were observed in metastatic tumors (Figures 3(d) and 3(e)) and primo vessels (Figure 3(f)). Phalloidin and DAPI staining revealed that these primo vessels expressed a linear arrangement of rod shape nuclei and positive phalloidin expression which conformed with the characters of primo vessels (Figure 3(g)). Accordant with our previous studies, the structure of primo vessels was obviously distinct from that of vascular vessels and lymphatic vessels.

Bottom Line: We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them.We deduced that primo vessels may be precursors of blood vessels.These results possibly provided a thoroughly new theoretic development in cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430070, China.

ABSTRACT
Gastric cancer is the fourth commonest cancer in the world and the second leading cause of cancer-related death. Investigation of gastric cancer metastasis is one of the hottest and major focuses in cancer research. Growing evidence manifested that primo vascular system (PVS) is a new kind of circulatory system beyond vascular and lymphatic system. Previous researches revealed that PVS is a specific tissue between endothelium and mesenchyme and is involved in cancer, especially in tumor metastasis and regeneration. In current study, we investigated the role of primo vessels in gastric cancer metastasis and its possible relationship to vascular vessels formation. Our results indicated that primo vessels were involved in gastric cancer metastasis. We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them. We deduced that primo vessels may be precursors of blood vessels. These results possibly provided a thoroughly new theoretic development in cancer metastasis.

No MeSH data available.


Related in: MedlinePlus