Limits...
Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis.

Ping A, Zhendong S, Rongmei Q, Jingxing D, Wei C, Zhongyin Z, Hesheng L, Soh KS - Evid Based Complement Alternat Med (2015)

Bottom Line: We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them.We deduced that primo vessels may be precursors of blood vessels.These results possibly provided a thoroughly new theoretic development in cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430070, China.

ABSTRACT
Gastric cancer is the fourth commonest cancer in the world and the second leading cause of cancer-related death. Investigation of gastric cancer metastasis is one of the hottest and major focuses in cancer research. Growing evidence manifested that primo vascular system (PVS) is a new kind of circulatory system beyond vascular and lymphatic system. Previous researches revealed that PVS is a specific tissue between endothelium and mesenchyme and is involved in cancer, especially in tumor metastasis and regeneration. In current study, we investigated the role of primo vessels in gastric cancer metastasis and its possible relationship to vascular vessels formation. Our results indicated that primo vessels were involved in gastric cancer metastasis. We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them. We deduced that primo vessels may be precursors of blood vessels. These results possibly provided a thoroughly new theoretic development in cancer metastasis.

No MeSH data available.


Related in: MedlinePlus

Orthotopic models in vivo and stable high level expression of GFP in gastric cancer metastatic tumors in nude mice. (a) and (b) 5 × 106 MKN-45-GFP cells were inoculated subcutaneously into the left flank of 5–7-week-old anesthetized nude mice. 6 weeks later, subcutaneous tumor grew obviously. (c) Subcutaneous tumor was harvested and incised into small fragments. (d) and (e) Tumor pieces were orthotopically implanted in the gastric wall with surgical suture. (f) and (g) Under the LZ12 microscope, gastric orthotopic tumors expressed strong GFP fluorescence. (h) GFP signals from subcutaneous tumor pieces were tested under fluorescence microscopy. (i) 6 to 8 weeks after the orthotopic implantation, gastric metastasis was investigated. Detection of GFP signal confirmed the metastatic tumors (arrows) and microtumors or gastric cancer cells (arrowheads).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4562087&req=5

fig2: Orthotopic models in vivo and stable high level expression of GFP in gastric cancer metastatic tumors in nude mice. (a) and (b) 5 × 106 MKN-45-GFP cells were inoculated subcutaneously into the left flank of 5–7-week-old anesthetized nude mice. 6 weeks later, subcutaneous tumor grew obviously. (c) Subcutaneous tumor was harvested and incised into small fragments. (d) and (e) Tumor pieces were orthotopically implanted in the gastric wall with surgical suture. (f) and (g) Under the LZ12 microscope, gastric orthotopic tumors expressed strong GFP fluorescence. (h) GFP signals from subcutaneous tumor pieces were tested under fluorescence microscopy. (i) 6 to 8 weeks after the orthotopic implantation, gastric metastasis was investigated. Detection of GFP signal confirmed the metastatic tumors (arrows) and microtumors or gastric cancer cells (arrowheads).

Mentions: MKN-45-GFP cells were collected at the log phase and injected subcutaneously into the mice at 107/0.2 mL. Six weeks later, tumors were harvested from the mice under anesthesia (by subcutaneous injection of 0.1 mL of solution of 0.04% Zoletil and 0.06% Rompun) and minced into small pieces (2 × 2 × 2 mm3) in HBSS containing 100 U/mL penicillin and 100 μg/mL streptomycin (Figures 2(a)–2(c)). For implantation, the mouse stomach was gently exteriorized via a left-side upper abdominal incision, and one small tissue pocket was formed in the middle wall of the greater curvature using microscissors. One tumor piece was placed into the pocket and closed with a 6-0 surgical suture (Figures 2(d) and 2(e)). Animals were kept in a sterile environment. All procedures of the operation described above were performed with an X7 magnification microscope (Olympus).


Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis.

Ping A, Zhendong S, Rongmei Q, Jingxing D, Wei C, Zhongyin Z, Hesheng L, Soh KS - Evid Based Complement Alternat Med (2015)

Orthotopic models in vivo and stable high level expression of GFP in gastric cancer metastatic tumors in nude mice. (a) and (b) 5 × 106 MKN-45-GFP cells were inoculated subcutaneously into the left flank of 5–7-week-old anesthetized nude mice. 6 weeks later, subcutaneous tumor grew obviously. (c) Subcutaneous tumor was harvested and incised into small fragments. (d) and (e) Tumor pieces were orthotopically implanted in the gastric wall with surgical suture. (f) and (g) Under the LZ12 microscope, gastric orthotopic tumors expressed strong GFP fluorescence. (h) GFP signals from subcutaneous tumor pieces were tested under fluorescence microscopy. (i) 6 to 8 weeks after the orthotopic implantation, gastric metastasis was investigated. Detection of GFP signal confirmed the metastatic tumors (arrows) and microtumors or gastric cancer cells (arrowheads).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562087&req=5

fig2: Orthotopic models in vivo and stable high level expression of GFP in gastric cancer metastatic tumors in nude mice. (a) and (b) 5 × 106 MKN-45-GFP cells were inoculated subcutaneously into the left flank of 5–7-week-old anesthetized nude mice. 6 weeks later, subcutaneous tumor grew obviously. (c) Subcutaneous tumor was harvested and incised into small fragments. (d) and (e) Tumor pieces were orthotopically implanted in the gastric wall with surgical suture. (f) and (g) Under the LZ12 microscope, gastric orthotopic tumors expressed strong GFP fluorescence. (h) GFP signals from subcutaneous tumor pieces were tested under fluorescence microscopy. (i) 6 to 8 weeks after the orthotopic implantation, gastric metastasis was investigated. Detection of GFP signal confirmed the metastatic tumors (arrows) and microtumors or gastric cancer cells (arrowheads).
Mentions: MKN-45-GFP cells were collected at the log phase and injected subcutaneously into the mice at 107/0.2 mL. Six weeks later, tumors were harvested from the mice under anesthesia (by subcutaneous injection of 0.1 mL of solution of 0.04% Zoletil and 0.06% Rompun) and minced into small pieces (2 × 2 × 2 mm3) in HBSS containing 100 U/mL penicillin and 100 μg/mL streptomycin (Figures 2(a)–2(c)). For implantation, the mouse stomach was gently exteriorized via a left-side upper abdominal incision, and one small tissue pocket was formed in the middle wall of the greater curvature using microscissors. One tumor piece was placed into the pocket and closed with a 6-0 surgical suture (Figures 2(d) and 2(e)). Animals were kept in a sterile environment. All procedures of the operation described above were performed with an X7 magnification microscope (Olympus).

Bottom Line: We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them.We deduced that primo vessels may be precursors of blood vessels.These results possibly provided a thoroughly new theoretic development in cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430070, China.

ABSTRACT
Gastric cancer is the fourth commonest cancer in the world and the second leading cause of cancer-related death. Investigation of gastric cancer metastasis is one of the hottest and major focuses in cancer research. Growing evidence manifested that primo vascular system (PVS) is a new kind of circulatory system beyond vascular and lymphatic system. Previous researches revealed that PVS is a specific tissue between endothelium and mesenchyme and is involved in cancer, especially in tumor metastasis and regeneration. In current study, we investigated the role of primo vessels in gastric cancer metastasis and its possible relationship to vascular vessels formation. Our results indicated that primo vessels were involved in gastric cancer metastasis. We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them. We deduced that primo vessels may be precursors of blood vessels. These results possibly provided a thoroughly new theoretic development in cancer metastasis.

No MeSH data available.


Related in: MedlinePlus