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Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis.

Ping A, Zhendong S, Rongmei Q, Jingxing D, Wei C, Zhongyin Z, Hesheng L, Soh KS - Evid Based Complement Alternat Med (2015)

Bottom Line: We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them.We deduced that primo vessels may be precursors of blood vessels.These results possibly provided a thoroughly new theoretic development in cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430070, China.

ABSTRACT
Gastric cancer is the fourth commonest cancer in the world and the second leading cause of cancer-related death. Investigation of gastric cancer metastasis is one of the hottest and major focuses in cancer research. Growing evidence manifested that primo vascular system (PVS) is a new kind of circulatory system beyond vascular and lymphatic system. Previous researches revealed that PVS is a specific tissue between endothelium and mesenchyme and is involved in cancer, especially in tumor metastasis and regeneration. In current study, we investigated the role of primo vessels in gastric cancer metastasis and its possible relationship to vascular vessels formation. Our results indicated that primo vessels were involved in gastric cancer metastasis. We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them. We deduced that primo vessels may be precursors of blood vessels. These results possibly provided a thoroughly new theoretic development in cancer metastasis.

No MeSH data available.


Related in: MedlinePlus

Isolation of stable, high GFP expressed MKN-45-GFP cells. (a) The enhanced GFP4 expression plasmid was transfected MKN-45 cells. The brightest fluorescent cells were sorted and cloned by flow cytometry. Stable high GFP-expression clones were isolated and cultured in the absence of G418 for 10 passages. (b) Stable high GFP-expression MKN-45-GFP cells cultured in the absence of G418 for 20 passages.
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fig1: Isolation of stable, high GFP expressed MKN-45-GFP cells. (a) The enhanced GFP4 expression plasmid was transfected MKN-45 cells. The brightest fluorescent cells were sorted and cloned by flow cytometry. Stable high GFP-expression clones were isolated and cultured in the absence of G418 for 10 passages. (b) Stable high GFP-expression MKN-45-GFP cells cultured in the absence of G418 for 20 passages.

Mentions: In the past, GFP has been used to detect metastatic cells in freshly excised tissue samples [12–14] and for studies of cell motility within primary tumors by in vivo time-lapse confocal microscopy [15]. Studies suggested that the use of GFP-expressing cells could improve in vivo investigations of the metastatic process. Utilization of GFP as a cell label for in vivo experiments requires that cells should be stably transfected and express GFP over a long term. The stable transfected MKN-45-GFP cells used in the present study exhibited a stable and strikingly bright fluorescence (screened by flow cytometry) in the absence of selective agent (G418) after numerous passages (Figure 1). This indicates the suitability and sensitivity of the MKN-45-GFP cells for long term use in vivo.


Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis.

Ping A, Zhendong S, Rongmei Q, Jingxing D, Wei C, Zhongyin Z, Hesheng L, Soh KS - Evid Based Complement Alternat Med (2015)

Isolation of stable, high GFP expressed MKN-45-GFP cells. (a) The enhanced GFP4 expression plasmid was transfected MKN-45 cells. The brightest fluorescent cells were sorted and cloned by flow cytometry. Stable high GFP-expression clones were isolated and cultured in the absence of G418 for 10 passages. (b) Stable high GFP-expression MKN-45-GFP cells cultured in the absence of G418 for 20 passages.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562087&req=5

fig1: Isolation of stable, high GFP expressed MKN-45-GFP cells. (a) The enhanced GFP4 expression plasmid was transfected MKN-45 cells. The brightest fluorescent cells were sorted and cloned by flow cytometry. Stable high GFP-expression clones were isolated and cultured in the absence of G418 for 10 passages. (b) Stable high GFP-expression MKN-45-GFP cells cultured in the absence of G418 for 20 passages.
Mentions: In the past, GFP has been used to detect metastatic cells in freshly excised tissue samples [12–14] and for studies of cell motility within primary tumors by in vivo time-lapse confocal microscopy [15]. Studies suggested that the use of GFP-expressing cells could improve in vivo investigations of the metastatic process. Utilization of GFP as a cell label for in vivo experiments requires that cells should be stably transfected and express GFP over a long term. The stable transfected MKN-45-GFP cells used in the present study exhibited a stable and strikingly bright fluorescence (screened by flow cytometry) in the absence of selective agent (G418) after numerous passages (Figure 1). This indicates the suitability and sensitivity of the MKN-45-GFP cells for long term use in vivo.

Bottom Line: We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them.We deduced that primo vessels may be precursors of blood vessels.These results possibly provided a thoroughly new theoretic development in cancer metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430070, China.

ABSTRACT
Gastric cancer is the fourth commonest cancer in the world and the second leading cause of cancer-related death. Investigation of gastric cancer metastasis is one of the hottest and major focuses in cancer research. Growing evidence manifested that primo vascular system (PVS) is a new kind of circulatory system beyond vascular and lymphatic system. Previous researches revealed that PVS is a specific tissue between endothelium and mesenchyme and is involved in cancer, especially in tumor metastasis and regeneration. In current study, we investigated the role of primo vessels in gastric cancer metastasis and its possible relationship to vascular vessels formation. Our results indicated that primo vessels were involved in gastric cancer metastasis. We observed blood vessel-mediated metastasis, primo vessel-mediated metastasis, and an intermediate state between them. We deduced that primo vessels may be precursors of blood vessels. These results possibly provided a thoroughly new theoretic development in cancer metastasis.

No MeSH data available.


Related in: MedlinePlus