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Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.


Immunolocalization of the AX1 heteroxylan epitope in cross-sections of developing and mature cotton fibers of FM966 (G. hirsutum) and JFW15 (G. arboreum). A double immunolocalization of the AX1 epitope at the inner and outer part of the cell wall is indicated by arrows in (C) and (F). Unmasking of the AX1 epitope in the primary cell wall of mature fibers is shown by arrows in (D), (E), (I) and (J). Striations of AX1 labeling can be seen in secondary cell walls in (H), (I) and (J). SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated; CW, Calcofluor White-stained sections. The scale is the same for all the images.
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pcv101-F6: Immunolocalization of the AX1 heteroxylan epitope in cross-sections of developing and mature cotton fibers of FM966 (G. hirsutum) and JFW15 (G. arboreum). A double immunolocalization of the AX1 epitope at the inner and outer part of the cell wall is indicated by arrows in (C) and (F). Unmasking of the AX1 epitope in the primary cell wall of mature fibers is shown by arrows in (D), (E), (I) and (J). Striations of AX1 labeling can be seen in secondary cell walls in (H), (I) and (J). SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated; CW, Calcofluor White-stained sections. The scale is the same for all the images.

Mentions: The AX1 heteroxylan epitope was detected in a punctate pattern around the primary cell wall of 17 dpa fibers of FM966 and JFW15 (Fig. 6). The already thickened secondary cell wall of JFW15 displayed AX1 labeling around the inner cell wall region next to the plasma membrane (Fig. 6A, F). This double localization of the AX1 epitope was also visible in FM966 at 25 dpa (Fig. 6C). In mature fibers, removal of pectin by pectate lyase uncovered the AX1 epitope in the primary cell wall (arrows in Fig. 6D, E, I, J). Additionally, in mature JFW15 fibers, the AX1 epitope was localized in concentric rings in the secondary cell wall (Fig. 6I, J). Similar detection of heteroxylan in the secondary cell wall of mature fibers was observed when using xylan-directed carbohydrate-binding modules (CBMs) CBM2b-1-2 and CBM22 (Fig. 7). The LM11 xylan antibody bound in a similar way, but the signal was weaker and the UX1 glucuronoxylan antibody bound mainly to the primary cell wall after pectin removal. The binding pattern in concentric rings was specific to the heteroxylan epitopes as verified by the LM19 HG antibody whose location was restricted to the primary cell wall of cotton fibers.Fig. 6


Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Immunolocalization of the AX1 heteroxylan epitope in cross-sections of developing and mature cotton fibers of FM966 (G. hirsutum) and JFW15 (G. arboreum). A double immunolocalization of the AX1 epitope at the inner and outer part of the cell wall is indicated by arrows in (C) and (F). Unmasking of the AX1 epitope in the primary cell wall of mature fibers is shown by arrows in (D), (E), (I) and (J). Striations of AX1 labeling can be seen in secondary cell walls in (H), (I) and (J). SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated; CW, Calcofluor White-stained sections. The scale is the same for all the images.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4562070&req=5

pcv101-F6: Immunolocalization of the AX1 heteroxylan epitope in cross-sections of developing and mature cotton fibers of FM966 (G. hirsutum) and JFW15 (G. arboreum). A double immunolocalization of the AX1 epitope at the inner and outer part of the cell wall is indicated by arrows in (C) and (F). Unmasking of the AX1 epitope in the primary cell wall of mature fibers is shown by arrows in (D), (E), (I) and (J). Striations of AX1 labeling can be seen in secondary cell walls in (H), (I) and (J). SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated; CW, Calcofluor White-stained sections. The scale is the same for all the images.
Mentions: The AX1 heteroxylan epitope was detected in a punctate pattern around the primary cell wall of 17 dpa fibers of FM966 and JFW15 (Fig. 6). The already thickened secondary cell wall of JFW15 displayed AX1 labeling around the inner cell wall region next to the plasma membrane (Fig. 6A, F). This double localization of the AX1 epitope was also visible in FM966 at 25 dpa (Fig. 6C). In mature fibers, removal of pectin by pectate lyase uncovered the AX1 epitope in the primary cell wall (arrows in Fig. 6D, E, I, J). Additionally, in mature JFW15 fibers, the AX1 epitope was localized in concentric rings in the secondary cell wall (Fig. 6I, J). Similar detection of heteroxylan in the secondary cell wall of mature fibers was observed when using xylan-directed carbohydrate-binding modules (CBMs) CBM2b-1-2 and CBM22 (Fig. 7). The LM11 xylan antibody bound in a similar way, but the signal was weaker and the UX1 glucuronoxylan antibody bound mainly to the primary cell wall after pectin removal. The binding pattern in concentric rings was specific to the heteroxylan epitopes as verified by the LM19 HG antibody whose location was restricted to the primary cell wall of cotton fibers.Fig. 6

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.