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Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.


Related in: MedlinePlus

Immunolocalization of heteromannans in cross-sections of mature cotton fibers. (A) Unmasking of the BS400-4 and LM21 heteromannan epitopes in equivalent sections of PimaS7 (G. barbadense) mature fibers after removal of pectic HG with pectate lyase. LM19 antibody is shown as a control of the pectate lyase enzymatic action. SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated. (B) The heteromannan BS400-4 epitope was found in all cotton species. Heteromannnans localized mainly at the primary cell wall and weakly in restricted regions of the secondary cell wall (arrowheads) and in the lumen (arrows) in FM966 (G. hirsutum), Krasnyj (G. herbaceum) and JFW15 (G. arboreum). All sections were SC/PL+ treated. (C) Mannanase treatment of PimaS7 mature fibers efficiently removed the heteromannan epitope BS400-4. All sections were SC/PL+ treated. All images in this figure were taken at the same time exposure (except for the Calcofluor White image in C). Exposure time in all BS400-4 and LM21 images = 1 s. The scale is the same for all the images.
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pcv101-F5: Immunolocalization of heteromannans in cross-sections of mature cotton fibers. (A) Unmasking of the BS400-4 and LM21 heteromannan epitopes in equivalent sections of PimaS7 (G. barbadense) mature fibers after removal of pectic HG with pectate lyase. LM19 antibody is shown as a control of the pectate lyase enzymatic action. SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated. (B) The heteromannan BS400-4 epitope was found in all cotton species. Heteromannnans localized mainly at the primary cell wall and weakly in restricted regions of the secondary cell wall (arrowheads) and in the lumen (arrows) in FM966 (G. hirsutum), Krasnyj (G. herbaceum) and JFW15 (G. arboreum). All sections were SC/PL+ treated. (C) Mannanase treatment of PimaS7 mature fibers efficiently removed the heteromannan epitope BS400-4. All sections were SC/PL+ treated. All images in this figure were taken at the same time exposure (except for the Calcofluor White image in C). Exposure time in all BS400-4 and LM21 images = 1 s. The scale is the same for all the images.

Mentions: The immunolocalization of heteromannan (LM21 and BS400-4) and heteroxylan (AX1, LM11, CBM2b2-1, CBM22 and UX1) epitopes in the cotton fibers was compared using cross-sections of resin-embedded material at several developmental stages. Masking of cell wall epitopes by non-related polysaccharides has been previously reported (Marcus et al. 2008, Marcus et al. 2010, Xue et al. 2013). On resin sections of mature cotton fibers, pre-treatment with sodium carbonate and pectate lyase uncovered heteromannan epitopes in the primary cell walls (Fig. 5A). Both the LM21 and BS400-4 heteromannan epitopes were found in the primary cell wall of mature fibers from all cotton lines and were highly masked by pectic HG (Fig. 5A, B). The BS400-4 probe showed higher binding to cotton fibers than the LM21probe (Fig. 5A), and PimaS7 (G. barbadense) had the highest fluorescence intensity at equivalent time exposures when compared with FM966 (G. hirsutum), Krasnyj (G. herbaceum) and JFW15 (G. arboreum) (Fig. 5B). This correlates well with the microarray data in which the LM21 epitope in mature fibers is detected at 40% of maximum signal in PimaS7 while it was below the detection limit in other lines. In situ labeling of developing PimaS7 fiber cross-sections (Supplementary Fig. S4) showed that the BS400-4 epitope is weakly detected in the secondary cell wall of 25 dpa fibers only, and pectate lyase treatment did not unmask the epitope in developing fibers.Fig. 5


Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Immunolocalization of heteromannans in cross-sections of mature cotton fibers. (A) Unmasking of the BS400-4 and LM21 heteromannan epitopes in equivalent sections of PimaS7 (G. barbadense) mature fibers after removal of pectic HG with pectate lyase. LM19 antibody is shown as a control of the pectate lyase enzymatic action. SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated. (B) The heteromannan BS400-4 epitope was found in all cotton species. Heteromannnans localized mainly at the primary cell wall and weakly in restricted regions of the secondary cell wall (arrowheads) and in the lumen (arrows) in FM966 (G. hirsutum), Krasnyj (G. herbaceum) and JFW15 (G. arboreum). All sections were SC/PL+ treated. (C) Mannanase treatment of PimaS7 mature fibers efficiently removed the heteromannan epitope BS400-4. All sections were SC/PL+ treated. All images in this figure were taken at the same time exposure (except for the Calcofluor White image in C). Exposure time in all BS400-4 and LM21 images = 1 s. The scale is the same for all the images.
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Related In: Results  -  Collection

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pcv101-F5: Immunolocalization of heteromannans in cross-sections of mature cotton fibers. (A) Unmasking of the BS400-4 and LM21 heteromannan epitopes in equivalent sections of PimaS7 (G. barbadense) mature fibers after removal of pectic HG with pectate lyase. LM19 antibody is shown as a control of the pectate lyase enzymatic action. SC, sodium carbonate; PL–, no pectate lyase treatment (only buffer); PL+, pectate lyase treated. (B) The heteromannan BS400-4 epitope was found in all cotton species. Heteromannnans localized mainly at the primary cell wall and weakly in restricted regions of the secondary cell wall (arrowheads) and in the lumen (arrows) in FM966 (G. hirsutum), Krasnyj (G. herbaceum) and JFW15 (G. arboreum). All sections were SC/PL+ treated. (C) Mannanase treatment of PimaS7 mature fibers efficiently removed the heteromannan epitope BS400-4. All sections were SC/PL+ treated. All images in this figure were taken at the same time exposure (except for the Calcofluor White image in C). Exposure time in all BS400-4 and LM21 images = 1 s. The scale is the same for all the images.
Mentions: The immunolocalization of heteromannan (LM21 and BS400-4) and heteroxylan (AX1, LM11, CBM2b2-1, CBM22 and UX1) epitopes in the cotton fibers was compared using cross-sections of resin-embedded material at several developmental stages. Masking of cell wall epitopes by non-related polysaccharides has been previously reported (Marcus et al. 2008, Marcus et al. 2010, Xue et al. 2013). On resin sections of mature cotton fibers, pre-treatment with sodium carbonate and pectate lyase uncovered heteromannan epitopes in the primary cell walls (Fig. 5A). Both the LM21 and BS400-4 heteromannan epitopes were found in the primary cell wall of mature fibers from all cotton lines and were highly masked by pectic HG (Fig. 5A, B). The BS400-4 probe showed higher binding to cotton fibers than the LM21probe (Fig. 5A), and PimaS7 (G. barbadense) had the highest fluorescence intensity at equivalent time exposures when compared with FM966 (G. hirsutum), Krasnyj (G. herbaceum) and JFW15 (G. arboreum) (Fig. 5B). This correlates well with the microarray data in which the LM21 epitope in mature fibers is detected at 40% of maximum signal in PimaS7 while it was below the detection limit in other lines. In situ labeling of developing PimaS7 fiber cross-sections (Supplementary Fig. S4) showed that the BS400-4 epitope is weakly detected in the secondary cell wall of 25 dpa fibers only, and pectate lyase treatment did not unmask the epitope in developing fibers.Fig. 5

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.


Related in: MedlinePlus