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Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.


Related in: MedlinePlus

Hierarchical clustering of transcripts associated with genes for mannan, xylan and cellulose synthases. RNA-seq data were filtered and clustered based on the expression level of transcripts identified as products of these genes based on GO annotations and publications. Right legend indicates identified homolog genes for the hits. The histogram on the right indicates the number of hits depending of their expression values (log2 transformed and split into five bins). (A) Clustering of mannan synthase gene expression. (B) Clustering of xylan synthase gene expression. (C) Clustering of CesA gene expression. Putative mannan or xylan synthase genes identified by gene ontology are named as GO for each cluster. CslD2_3 means that the gene was identified in our sample from two transcripts (splicing variants), one being a ortholog of CslD2 transcripts and one being a ortholog of CslD3.
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pcv101-F3: Hierarchical clustering of transcripts associated with genes for mannan, xylan and cellulose synthases. RNA-seq data were filtered and clustered based on the expression level of transcripts identified as products of these genes based on GO annotations and publications. Right legend indicates identified homolog genes for the hits. The histogram on the right indicates the number of hits depending of their expression values (log2 transformed and split into five bins). (A) Clustering of mannan synthase gene expression. (B) Clustering of xylan synthase gene expression. (C) Clustering of CesA gene expression. Putative mannan or xylan synthase genes identified by gene ontology are named as GO for each cluster. CslD2_3 means that the gene was identified in our sample from two transcripts (splicing variants), one being a ortholog of CslD2 transcripts and one being a ortholog of CslD3.

Mentions: The presence of mannan and xylan in cotton fiber cell walls suggests that mannan- and xylan-related genes are expressed in the developing fiber. Therefore, transcriptomic data from 10, 15 and 20 dpa cotton fibers were analyzed for the presence of mannan- and xylan-related transcripts. We identified five mannan synthesis-associated genes (Verhertbruggen et al. 2011, Yin et al. 2011) from the cellulose synthase-like (Csl) family (CslA2, CslA9, CslD2, CslD3 and CslD5) and three gene ontology (GO) annotated genes that showed expression in all the studied cotton lines (Fig. 3A). CslA2, CslD2 and CslD3 homologs appear to be expressed at all time points, whereas the CslA9 and CslD5 homologs are only expressed at 10 dpa. Amongst the genes expressed at all three time points, the two CslA2 homologs and one CslD3 homolog have a stronger expression at early days post-anthesis (particularly at 10 dpa). Gossypium barbadense and G. hirsutum showed a higher expression of the CslA2 homolog at both 10 and 15 dpa, while CslA2 is primarily expressed at 10 dpa in G. arboreum. The opposite is observed for the CslD3 homolog, which is expressed at both 10 and 15 dpa in G. arboreum but only at 10 dpa in the two other species. Xylan transcripts showed a completely different pattern of expression compared with mannan-related transcripts, with a strong signal only at 15 and 20 dpa (Fig. 3B). A block of expression is clearly visible in all species over 15 and 20 dpa, including genes homologous to IRX9, IRX10, GUX1, GUX2 and FRA8, and GO annotated genes. However, certain genes had a higher expression in G. arboreum compared with the two other species, and are more strongly expressed at 20 dpa in G. barbadense. Expression profiles of the cellulose synthase (CesA) genes were used as a positive control for the fiber transcriptome data (Fig. 3C). For the secondary cell wall-related CesA genes, including the CesA4, 7 and 8 homologs, a block of expression similar to that of xylan-related genes was observed at 15 and 20 dpa, with a stronger expression at 20 dpa in the G. barbadense lines.Fig. 3


Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Hierarchical clustering of transcripts associated with genes for mannan, xylan and cellulose synthases. RNA-seq data were filtered and clustered based on the expression level of transcripts identified as products of these genes based on GO annotations and publications. Right legend indicates identified homolog genes for the hits. The histogram on the right indicates the number of hits depending of their expression values (log2 transformed and split into five bins). (A) Clustering of mannan synthase gene expression. (B) Clustering of xylan synthase gene expression. (C) Clustering of CesA gene expression. Putative mannan or xylan synthase genes identified by gene ontology are named as GO for each cluster. CslD2_3 means that the gene was identified in our sample from two transcripts (splicing variants), one being a ortholog of CslD2 transcripts and one being a ortholog of CslD3.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4562070&req=5

pcv101-F3: Hierarchical clustering of transcripts associated with genes for mannan, xylan and cellulose synthases. RNA-seq data were filtered and clustered based on the expression level of transcripts identified as products of these genes based on GO annotations and publications. Right legend indicates identified homolog genes for the hits. The histogram on the right indicates the number of hits depending of their expression values (log2 transformed and split into five bins). (A) Clustering of mannan synthase gene expression. (B) Clustering of xylan synthase gene expression. (C) Clustering of CesA gene expression. Putative mannan or xylan synthase genes identified by gene ontology are named as GO for each cluster. CslD2_3 means that the gene was identified in our sample from two transcripts (splicing variants), one being a ortholog of CslD2 transcripts and one being a ortholog of CslD3.
Mentions: The presence of mannan and xylan in cotton fiber cell walls suggests that mannan- and xylan-related genes are expressed in the developing fiber. Therefore, transcriptomic data from 10, 15 and 20 dpa cotton fibers were analyzed for the presence of mannan- and xylan-related transcripts. We identified five mannan synthesis-associated genes (Verhertbruggen et al. 2011, Yin et al. 2011) from the cellulose synthase-like (Csl) family (CslA2, CslA9, CslD2, CslD3 and CslD5) and three gene ontology (GO) annotated genes that showed expression in all the studied cotton lines (Fig. 3A). CslA2, CslD2 and CslD3 homologs appear to be expressed at all time points, whereas the CslA9 and CslD5 homologs are only expressed at 10 dpa. Amongst the genes expressed at all three time points, the two CslA2 homologs and one CslD3 homolog have a stronger expression at early days post-anthesis (particularly at 10 dpa). Gossypium barbadense and G. hirsutum showed a higher expression of the CslA2 homolog at both 10 and 15 dpa, while CslA2 is primarily expressed at 10 dpa in G. arboreum. The opposite is observed for the CslD3 homolog, which is expressed at both 10 and 15 dpa in G. arboreum but only at 10 dpa in the two other species. Xylan transcripts showed a completely different pattern of expression compared with mannan-related transcripts, with a strong signal only at 15 and 20 dpa (Fig. 3B). A block of expression is clearly visible in all species over 15 and 20 dpa, including genes homologous to IRX9, IRX10, GUX1, GUX2 and FRA8, and GO annotated genes. However, certain genes had a higher expression in G. arboreum compared with the two other species, and are more strongly expressed at 20 dpa in G. barbadense. Expression profiles of the cellulose synthase (CesA) genes were used as a positive control for the fiber transcriptome data (Fig. 3C). For the secondary cell wall-related CesA genes, including the CesA4, 7 and 8 homologs, a block of expression similar to that of xylan-related genes was observed at 15 and 20 dpa, with a stronger expression at 20 dpa in the G. barbadense lines.Fig. 3

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.


Related in: MedlinePlus