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Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.


Glycan microarray of CDTA and NaOH extractions of cotton fibers at 11 different developmental stages from four different cotton species. Monoclonal antibodies used were AX1 for heteroxylan, LM21 for heteromannan and JIM7 for esterified homogalacturonan as a control for the CDTA extraction. DPA, days post-anthesis; Mat, mature. Values are means of nine technical replicates scaled to one maximum value of 100 for each antibody.
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pcv101-F1: Glycan microarray of CDTA and NaOH extractions of cotton fibers at 11 different developmental stages from four different cotton species. Monoclonal antibodies used were AX1 for heteroxylan, LM21 for heteromannan and JIM7 for esterified homogalacturonan as a control for the CDTA extraction. DPA, days post-anthesis; Mat, mature. Values are means of nine technical replicates scaled to one maximum value of 100 for each antibody.

Mentions: In order to study the occurrence of the heteromannan and arabinoxylan epitopes in developing and mature fibers, cotton fiber cell walls were sequentially extracted and screened by glycan microarrays providing semi-quantitative data (Moller et al. 2007). 1,2-Diaminocyclohexanetetraacetic acid (CDTA) and NaOH extractions of G. barbadense (PimaS7), G. hirsutum (FM966), G. herbaceum (Krasnyj) and G. arboreum (JFW15) fibers at 11 different developmental stages resulted in the detection of the LM21 heteromannan and AX1 heteroxylan epitopes in the alkali cell wall extracts (Fig. 1). The JIM7 high-ester homogalacturonan (HG) probe was included as control for the CDTA cell wall extraction efficiency.Fig. 1


Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.

Hernandez-Gomez MC, Runavot JL, Guo X, Bourot S, Benians TA, Willats WG, Meulewaeter F, Knox JP - Plant Cell Physiol. (2015)

Glycan microarray of CDTA and NaOH extractions of cotton fibers at 11 different developmental stages from four different cotton species. Monoclonal antibodies used were AX1 for heteroxylan, LM21 for heteromannan and JIM7 for esterified homogalacturonan as a control for the CDTA extraction. DPA, days post-anthesis; Mat, mature. Values are means of nine technical replicates scaled to one maximum value of 100 for each antibody.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562070&req=5

pcv101-F1: Glycan microarray of CDTA and NaOH extractions of cotton fibers at 11 different developmental stages from four different cotton species. Monoclonal antibodies used were AX1 for heteroxylan, LM21 for heteromannan and JIM7 for esterified homogalacturonan as a control for the CDTA extraction. DPA, days post-anthesis; Mat, mature. Values are means of nine technical replicates scaled to one maximum value of 100 for each antibody.
Mentions: In order to study the occurrence of the heteromannan and arabinoxylan epitopes in developing and mature fibers, cotton fiber cell walls were sequentially extracted and screened by glycan microarrays providing semi-quantitative data (Moller et al. 2007). 1,2-Diaminocyclohexanetetraacetic acid (CDTA) and NaOH extractions of G. barbadense (PimaS7), G. hirsutum (FM966), G. herbaceum (Krasnyj) and G. arboreum (JFW15) fibers at 11 different developmental stages resulted in the detection of the LM21 heteromannan and AX1 heteroxylan epitopes in the alkali cell wall extracts (Fig. 1). The JIM7 high-ester homogalacturonan (HG) probe was included as control for the CDTA cell wall extraction efficiency.Fig. 1

Bottom Line: In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber.These developmental dynamics were supported by transcript profiling of biosynthetic genes.Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls.

View Article: PubMed Central - PubMed

Affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK These authors contributed equally to this work.

No MeSH data available.