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Use of an Extract of Indian Sacred Plant Ocimum sanctum as an Anticariogenic Agent: An in vitro Study.

Kochikar Pai R, Bhat SS, Salman A, Chandra J - Int J Clin Pediatr Dent (2015)

Bottom Line: Ocimum sanctum leaf extract was effective against all the microorganisms.Use of an Extract of Indian Sacred Plant Ocimum Sanctum as an Anticariogenic Agent: An in vitro Study.Int J Clin Pediatr Dent 2015;8(2):99-101.

View Article: PubMed Central - PubMed

Affiliation: Reader, Department of Pedodontics and Preventive Dentistry Yenepoya Dental College, Mangalore, Karnataka, India.

ABSTRACT

Aim: To analyze the efficacy of three different concentrations of Ocimum sanctum extract against various microorganisms, Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus acidophilus.

Materials and methods: Ethanolic extract of Ocimum sanctum was prepared by the hot extraction method. The extract was diluted with an inert solvent, dimethyl sulfoxide to obtain 3 different concentrations (2.5, 5 and 10%) of the extract. 0.2% chlorhexidine was used as a positive control and dimethyl sul-foxide was used as a negative control. The extract, along with the controls, was then subjected to microbiological investigation to determine which concentration among the 3 different concentrations of extract gave a wider inhibition zone against S. mutans, S. mitis, S. sanguis, L. acidophilus. The zones of inhibition were measured in millimeters.

Results: Ocimum sanctum leaf extract demonstrated maximum antimicrobial activity against microorganisms responsible for dental caries at the 10% concentration level although 5 and 2.5% were also effective. Maximum activity was seen against S. mutans and S. sanguis with 10% extract.

Conclusion: Ocimum sanctum leaf extract was effective against all the microorganisms. How to cite this article: Pai RK, Bhat SS, Salman A, Chandra J. Use of an Extract of Indian Sacred Plant Ocimum Sanctum as an Anticariogenic Agent: An in vitro Study. Int J Clin Pediatr Dent 2015;8(2):99-101.

No MeSH data available.


Related in: MedlinePlus

Zone of inhibition for S. mutans
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Figure 1: Zone of inhibition for S. mutans

Mentions: The data obtained were appraised observationally. All the bacterial strains were resistant to dimethyl sulfoxide. For 0.2 % chlorhexidine (positive control) a wider zone of inhibition of 28 mm was achieved for S. mutans (Fig. 1), followed by 24, 23 and 22 mm for L. acidophilus, S. sanguis and S. mitis respectively (Table 1). Among the different concentrations of O. sanctum extract used, 10% extract showed a wider zone when compared to 5% and 2.5 % extract. Maximum activity was seen against S. mutans and S. sanguis with 10% extract.


Use of an Extract of Indian Sacred Plant Ocimum sanctum as an Anticariogenic Agent: An in vitro Study.

Kochikar Pai R, Bhat SS, Salman A, Chandra J - Int J Clin Pediatr Dent (2015)

Zone of inhibition for S. mutans
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4562040&req=5

Figure 1: Zone of inhibition for S. mutans
Mentions: The data obtained were appraised observationally. All the bacterial strains were resistant to dimethyl sulfoxide. For 0.2 % chlorhexidine (positive control) a wider zone of inhibition of 28 mm was achieved for S. mutans (Fig. 1), followed by 24, 23 and 22 mm for L. acidophilus, S. sanguis and S. mitis respectively (Table 1). Among the different concentrations of O. sanctum extract used, 10% extract showed a wider zone when compared to 5% and 2.5 % extract. Maximum activity was seen against S. mutans and S. sanguis with 10% extract.

Bottom Line: Ocimum sanctum leaf extract was effective against all the microorganisms.Use of an Extract of Indian Sacred Plant Ocimum Sanctum as an Anticariogenic Agent: An in vitro Study.Int J Clin Pediatr Dent 2015;8(2):99-101.

View Article: PubMed Central - PubMed

Affiliation: Reader, Department of Pedodontics and Preventive Dentistry Yenepoya Dental College, Mangalore, Karnataka, India.

ABSTRACT

Aim: To analyze the efficacy of three different concentrations of Ocimum sanctum extract against various microorganisms, Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, Lactobacillus acidophilus.

Materials and methods: Ethanolic extract of Ocimum sanctum was prepared by the hot extraction method. The extract was diluted with an inert solvent, dimethyl sulfoxide to obtain 3 different concentrations (2.5, 5 and 10%) of the extract. 0.2% chlorhexidine was used as a positive control and dimethyl sul-foxide was used as a negative control. The extract, along with the controls, was then subjected to microbiological investigation to determine which concentration among the 3 different concentrations of extract gave a wider inhibition zone against S. mutans, S. mitis, S. sanguis, L. acidophilus. The zones of inhibition were measured in millimeters.

Results: Ocimum sanctum leaf extract demonstrated maximum antimicrobial activity against microorganisms responsible for dental caries at the 10% concentration level although 5 and 2.5% were also effective. Maximum activity was seen against S. mutans and S. sanguis with 10% extract.

Conclusion: Ocimum sanctum leaf extract was effective against all the microorganisms. How to cite this article: Pai RK, Bhat SS, Salman A, Chandra J. Use of an Extract of Indian Sacred Plant Ocimum Sanctum as an Anticariogenic Agent: An in vitro Study. Int J Clin Pediatr Dent 2015;8(2):99-101.

No MeSH data available.


Related in: MedlinePlus