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Experimental infections of rabbits with proliferative and latent stages of Besnoitia besnoiti.

Liénard E, Pop L, Prevot F, Grisez C, Mallet V, Raymond-Letron I, Bouhsira É, Franc M, Jacquiet P - Parasitol. Res. (2015)

Bottom Line: Clinical follow-up and blood sampling for serological survey and qPCR were performed during 10 weeks until euthanasia.Seroconversion occurred in group T without any clinical signs.The proposed model could be used to assess the in vivo effectiveness of vaccine or drugs against cattle besnoitiosis.

View Article: PubMed Central - PubMed

Affiliation: UMR INRA/DGER 1225, INP - École Nationale Vétérinaire de Toulouse, Laboratoire de Parasitologie, 23 chemin des Capelles, F-31076, Toulouse, France, e.lienard@envt.fr.

ABSTRACT
Cattle besnoitiosis due to Besnoitia besnoiti is spreading across Europe and is responsible for severe economic losses in newly infected herds. Experimentally speaking, rabbits have been found to be susceptible to this parasite. The adaptation of B. besnoiti to rabbits may offer a new, easier and cheaper model of investigation for this disease. This study compared the virulence between tachyzoites and bradyzoites of B. besnoiti in rabbits. Eighteen New Zealand rabbits were allocated into three groups of six animals each. The rabbits from the control (group C), "tachyzoite" (group T) and "bradyzoite" (group B) groups were subcutaneously injected in the right flank with 66 μg of ovalbumin, 6.10(6) tachyzoites (125th passage on Vero cells) and 6.10(6) bradyzoites (collected from a natural infected cow) of B. besnoiti, respectively. Clinical follow-up and blood sampling for serological survey and qPCR were performed during 10 weeks until euthanasia. Molecular and immunohistochemistry examination was achieved on 25 samples of tissue per rabbit. Seroconversion occurred in group T without any clinical signs. Rabbits of group B exhibited a febrile condition (temperature above 40 °C from day 8 to day 11 following injection) with positive qPCR in blood. Cysts of B. besnoiti were found on skin samples and organs of rabbits from group B in tissue explored with threshold cycle (Ct) values below 30. These results suggest a higher virulence of bradyzoites in rabbits than Vero cell-cultivated tachyzoites. The proposed model could be used to assess the in vivo effectiveness of vaccine or drugs against cattle besnoitiosis.

No MeSH data available.


Related in: MedlinePlus

Histopathological identification of B. besnoiti cyst (haematoxylin and eosin stain, ×1000). The sample was taken at 70 dpi from the fore leg dermis of the B05 rabbit. Tissue cyst of B. besnoiti in collagenic dermis was spherical in shape, thick walled and contained a single maximally developed parasitophorous vacuole with thousands of bradyzoites. Note the several host cell nuclei and nucleoli. A granulomatous inflammatory infiltrate (predominantly macrophages and lymphocytes) surrounded the cyst. Bar = 10 μm
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Fig4: Histopathological identification of B. besnoiti cyst (haematoxylin and eosin stain, ×1000). The sample was taken at 70 dpi from the fore leg dermis of the B05 rabbit. Tissue cyst of B. besnoiti in collagenic dermis was spherical in shape, thick walled and contained a single maximally developed parasitophorous vacuole with thousands of bradyzoites. Note the several host cell nuclei and nucleoli. A granulomatous inflammatory infiltrate (predominantly macrophages and lymphocytes) surrounded the cyst. Bar = 10 μm

Mentions: Cysts were recovered in the 11 samples exhibiting Ct values below 30 (tables 3 and 4) in group B and corresponding to the fore and hind leg, the right flank, the nasal mucosa, the right eyelid and the penis (Fig. 4). They were readily identified as B. besnoiti cysts with anti-B. besnoiti immunohistochemistry (Fig. 5).Fig. 4


Experimental infections of rabbits with proliferative and latent stages of Besnoitia besnoiti.

Liénard E, Pop L, Prevot F, Grisez C, Mallet V, Raymond-Letron I, Bouhsira É, Franc M, Jacquiet P - Parasitol. Res. (2015)

Histopathological identification of B. besnoiti cyst (haematoxylin and eosin stain, ×1000). The sample was taken at 70 dpi from the fore leg dermis of the B05 rabbit. Tissue cyst of B. besnoiti in collagenic dermis was spherical in shape, thick walled and contained a single maximally developed parasitophorous vacuole with thousands of bradyzoites. Note the several host cell nuclei and nucleoli. A granulomatous inflammatory infiltrate (predominantly macrophages and lymphocytes) surrounded the cyst. Bar = 10 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4562009&req=5

Fig4: Histopathological identification of B. besnoiti cyst (haematoxylin and eosin stain, ×1000). The sample was taken at 70 dpi from the fore leg dermis of the B05 rabbit. Tissue cyst of B. besnoiti in collagenic dermis was spherical in shape, thick walled and contained a single maximally developed parasitophorous vacuole with thousands of bradyzoites. Note the several host cell nuclei and nucleoli. A granulomatous inflammatory infiltrate (predominantly macrophages and lymphocytes) surrounded the cyst. Bar = 10 μm
Mentions: Cysts were recovered in the 11 samples exhibiting Ct values below 30 (tables 3 and 4) in group B and corresponding to the fore and hind leg, the right flank, the nasal mucosa, the right eyelid and the penis (Fig. 4). They were readily identified as B. besnoiti cysts with anti-B. besnoiti immunohistochemistry (Fig. 5).Fig. 4

Bottom Line: Clinical follow-up and blood sampling for serological survey and qPCR were performed during 10 weeks until euthanasia.Seroconversion occurred in group T without any clinical signs.The proposed model could be used to assess the in vivo effectiveness of vaccine or drugs against cattle besnoitiosis.

View Article: PubMed Central - PubMed

Affiliation: UMR INRA/DGER 1225, INP - École Nationale Vétérinaire de Toulouse, Laboratoire de Parasitologie, 23 chemin des Capelles, F-31076, Toulouse, France, e.lienard@envt.fr.

ABSTRACT
Cattle besnoitiosis due to Besnoitia besnoiti is spreading across Europe and is responsible for severe economic losses in newly infected herds. Experimentally speaking, rabbits have been found to be susceptible to this parasite. The adaptation of B. besnoiti to rabbits may offer a new, easier and cheaper model of investigation for this disease. This study compared the virulence between tachyzoites and bradyzoites of B. besnoiti in rabbits. Eighteen New Zealand rabbits were allocated into three groups of six animals each. The rabbits from the control (group C), "tachyzoite" (group T) and "bradyzoite" (group B) groups were subcutaneously injected in the right flank with 66 μg of ovalbumin, 6.10(6) tachyzoites (125th passage on Vero cells) and 6.10(6) bradyzoites (collected from a natural infected cow) of B. besnoiti, respectively. Clinical follow-up and blood sampling for serological survey and qPCR were performed during 10 weeks until euthanasia. Molecular and immunohistochemistry examination was achieved on 25 samples of tissue per rabbit. Seroconversion occurred in group T without any clinical signs. Rabbits of group B exhibited a febrile condition (temperature above 40 °C from day 8 to day 11 following injection) with positive qPCR in blood. Cysts of B. besnoiti were found on skin samples and organs of rabbits from group B in tissue explored with threshold cycle (Ct) values below 30. These results suggest a higher virulence of bradyzoites in rabbits than Vero cell-cultivated tachyzoites. The proposed model could be used to assess the in vivo effectiveness of vaccine or drugs against cattle besnoitiosis.

No MeSH data available.


Related in: MedlinePlus