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Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.


Comparison of the density and degranulation ratio of MCs identified in Hemacolor-stained scPVS and osPVS tissue. ((a) and (b)) Representative photomicrographs showing the distribution of MCs in the PNs of scPVS and osPVS stained with Hemacolor. ((c) and (d)) Summary bar graphs showing the number of MCs (c) and the degranulation ratio of these MCs in the scPVS and osPVS tissue (d). PVS cells counted from 21 fields (100 × 100 μm) in an image of the Hemacolor staining of the PVS tissue (n = 7) at magnifications of 400 and 1,000x.
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fig10: Comparison of the density and degranulation ratio of MCs identified in Hemacolor-stained scPVS and osPVS tissue. ((a) and (b)) Representative photomicrographs showing the distribution of MCs in the PNs of scPVS and osPVS stained with Hemacolor. ((c) and (d)) Summary bar graphs showing the number of MCs (c) and the degranulation ratio of these MCs in the scPVS and osPVS tissue (d). PVS cells counted from 21 fields (100 × 100 μm) in an image of the Hemacolor staining of the PVS tissue (n = 7) at magnifications of 400 and 1,000x.

Mentions: The above results regarding gross morphology, cytomorphology, and structural properties have many features in common with the well-established PVS [2, 5, 6]; thus, the subcutaneous threadlike structure, vessel, and node part were identified as the scPVS, the PV, and the PN, respectively. In addition, the large granular cells and small round cells of the threadlike structure were also identified as the MCs and WBCs of the PVS reported previously in the osPVS [6]. Figure 10 shows a comparison of the density and degranulation ratios of the MCs in the scPVS and osPVS tissues stained by Hemacolor. The cell densities of the MCs in the scPVS were lower than that of the osPVS (Figures 10(a) and 10(b)), and the degranulation ratio of the MCs in the scPVS was higher than the ratio in the osPVS (Figures 10(c) and 10(d)), which indicates that the scPVS is different from the osPVS in MC activation.


Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Comparison of the density and degranulation ratio of MCs identified in Hemacolor-stained scPVS and osPVS tissue. ((a) and (b)) Representative photomicrographs showing the distribution of MCs in the PNs of scPVS and osPVS stained with Hemacolor. ((c) and (d)) Summary bar graphs showing the number of MCs (c) and the degranulation ratio of these MCs in the scPVS and osPVS tissue (d). PVS cells counted from 21 fields (100 × 100 μm) in an image of the Hemacolor staining of the PVS tissue (n = 7) at magnifications of 400 and 1,000x.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4561983&req=5

fig10: Comparison of the density and degranulation ratio of MCs identified in Hemacolor-stained scPVS and osPVS tissue. ((a) and (b)) Representative photomicrographs showing the distribution of MCs in the PNs of scPVS and osPVS stained with Hemacolor. ((c) and (d)) Summary bar graphs showing the number of MCs (c) and the degranulation ratio of these MCs in the scPVS and osPVS tissue (d). PVS cells counted from 21 fields (100 × 100 μm) in an image of the Hemacolor staining of the PVS tissue (n = 7) at magnifications of 400 and 1,000x.
Mentions: The above results regarding gross morphology, cytomorphology, and structural properties have many features in common with the well-established PVS [2, 5, 6]; thus, the subcutaneous threadlike structure, vessel, and node part were identified as the scPVS, the PV, and the PN, respectively. In addition, the large granular cells and small round cells of the threadlike structure were also identified as the MCs and WBCs of the PVS reported previously in the osPVS [6]. Figure 10 shows a comparison of the density and degranulation ratios of the MCs in the scPVS and osPVS tissues stained by Hemacolor. The cell densities of the MCs in the scPVS were lower than that of the osPVS (Figures 10(a) and 10(b)), and the degranulation ratio of the MCs in the scPVS was higher than the ratio in the osPVS (Figures 10(c) and 10(d)), which indicates that the scPVS is different from the osPVS in MC activation.

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.