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Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.


Related in: MedlinePlus

Comparison of the subcutaneous threadlike structure and lymphatic vessel using transmission electron microscopy. (a) Threadlike structure containing a sinus structure (S), mast cell (MC) and its granules (asterisks), monocyte (M), and eosinophil (E). (b) Lymphatic vessel containing mostly lymphocytes (L). Note that there is a difference in the major resident cells between the threadlike structure and the lymphatic vessel.
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fig9: Comparison of the subcutaneous threadlike structure and lymphatic vessel using transmission electron microscopy. (a) Threadlike structure containing a sinus structure (S), mast cell (MC) and its granules (asterisks), monocyte (M), and eosinophil (E). (b) Lymphatic vessel containing mostly lymphocytes (L). Note that there is a difference in the major resident cells between the threadlike structure and the lymphatic vessel.

Mentions: Electron microscopy was used to show differences between the threadlike structure and the lymphatic vessel in isolation (Figure 8). The threadlike structure was comprised of a bundle structure of several subducts (Figure 8(a)), whereas the lymphatic vessel did not appear to have bundle structure (Figure 8(c)). At a higher magnification view, we observed the round cells (Figure 8(b), asterisks) and fine fiber structures on the surface of the threadlike structure. In contrast, in the lymphatic vessel, such cells were not found and the fiber structures were more extensive than those of threadlike structure (Figures 8(b) and 8(d)). Figure 9 shows the major resident cells of the threadlike structure and the lymphatic vessel revealed by transmission electron microscopy. As can be seen, the threadlike structure contains various immune cells, including MCs, eosinophil, and granules (Figure 9(a)), whereas the lymphatic vessel is mostly comprised of lymphocytes (Figure 9(b)). Thus, there are clear differences between the threadlike structure and lymphatic vessel in terms of the presence or absence of the bundle structures and the types of resident cells.


Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Comparison of the subcutaneous threadlike structure and lymphatic vessel using transmission electron microscopy. (a) Threadlike structure containing a sinus structure (S), mast cell (MC) and its granules (asterisks), monocyte (M), and eosinophil (E). (b) Lymphatic vessel containing mostly lymphocytes (L). Note that there is a difference in the major resident cells between the threadlike structure and the lymphatic vessel.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4561983&req=5

fig9: Comparison of the subcutaneous threadlike structure and lymphatic vessel using transmission electron microscopy. (a) Threadlike structure containing a sinus structure (S), mast cell (MC) and its granules (asterisks), monocyte (M), and eosinophil (E). (b) Lymphatic vessel containing mostly lymphocytes (L). Note that there is a difference in the major resident cells between the threadlike structure and the lymphatic vessel.
Mentions: Electron microscopy was used to show differences between the threadlike structure and the lymphatic vessel in isolation (Figure 8). The threadlike structure was comprised of a bundle structure of several subducts (Figure 8(a)), whereas the lymphatic vessel did not appear to have bundle structure (Figure 8(c)). At a higher magnification view, we observed the round cells (Figure 8(b), asterisks) and fine fiber structures on the surface of the threadlike structure. In contrast, in the lymphatic vessel, such cells were not found and the fiber structures were more extensive than those of threadlike structure (Figures 8(b) and 8(d)). Figure 9 shows the major resident cells of the threadlike structure and the lymphatic vessel revealed by transmission electron microscopy. As can be seen, the threadlike structure contains various immune cells, including MCs, eosinophil, and granules (Figure 9(a)), whereas the lymphatic vessel is mostly comprised of lymphocytes (Figure 9(b)). Thus, there are clear differences between the threadlike structure and lymphatic vessel in terms of the presence or absence of the bundle structures and the types of resident cells.

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.


Related in: MedlinePlus