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Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.


The inner space structure containing cells along the inside of the subcutaneous threadlike structure. (a) Continuous inner space (arrows) along the longitudinal axis of a threadlike structure revealed by Hemacolor staining. (b) Distribution of the cells (large granular cells, arrowheads; granules, dotted circle) in the inner space (marked as “b” in (a), 20–30 μm) of the vessel. (c) Large inner space structure (> 50 μm) inside the subcutaneous threadlike structure showing low cellularity revealed by Hemacolor staining. (d) Distribution of the cells (small round cells, arrowheads) in the inner space (marked as “d” in (c)) of the vessel. (e) Cross-sectional image (200 μm) showing an inner space (dotted square, > 50 μm) of a threadlike structure revealed by Hemacolor staining. (f) Inner space (marked as “f” in (e)) within a vessel slice containing granules (about 1 μm, inset). Note that the inner space contains the resident cells of the threadlike structure.
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fig7: The inner space structure containing cells along the inside of the subcutaneous threadlike structure. (a) Continuous inner space (arrows) along the longitudinal axis of a threadlike structure revealed by Hemacolor staining. (b) Distribution of the cells (large granular cells, arrowheads; granules, dotted circle) in the inner space (marked as “b” in (a), 20–30 μm) of the vessel. (c) Large inner space structure (> 50 μm) inside the subcutaneous threadlike structure showing low cellularity revealed by Hemacolor staining. (d) Distribution of the cells (small round cells, arrowheads) in the inner space (marked as “d” in (c)) of the vessel. (e) Cross-sectional image (200 μm) showing an inner space (dotted square, > 50 μm) of a threadlike structure revealed by Hemacolor staining. (f) Inner space (marked as “f” in (e)) within a vessel slice containing granules (about 1 μm, inset). Note that the inner space contains the resident cells of the threadlike structure.

Mentions: The inner regions of the vessel of the threadlike structure revealed by Hemacolor staining appeared as white space, indicating a lower cellularity (Figure 7). The inner space was linearly continuous along the longitudinal axis of the vessel (Figures 7(a) and 7(b)). The diameters of the inner space were 20–60 μm. The inner space structures contained various cells, such as large granular cells (Figure 7(b), arrowheads), granules (Figure 7(b), dotted circle), and small round cells (Figure 7(d), arrowheads). Figure 7(c) shows a bundle-like structure revealed by Hemacolor staining as shown in Figure 3 by scanning electron microscopy. In addition, a cross-sectioned slice (200 μm) of the threadlike structure was stained, and the small and large granular cells were observed in the whole threadlike structures. As shown in Figures 7(e) and 7(f), there was a sinus (~60 μm) that contained granules (~1 μm).


Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

The inner space structure containing cells along the inside of the subcutaneous threadlike structure. (a) Continuous inner space (arrows) along the longitudinal axis of a threadlike structure revealed by Hemacolor staining. (b) Distribution of the cells (large granular cells, arrowheads; granules, dotted circle) in the inner space (marked as “b” in (a), 20–30 μm) of the vessel. (c) Large inner space structure (> 50 μm) inside the subcutaneous threadlike structure showing low cellularity revealed by Hemacolor staining. (d) Distribution of the cells (small round cells, arrowheads) in the inner space (marked as “d” in (c)) of the vessel. (e) Cross-sectional image (200 μm) showing an inner space (dotted square, > 50 μm) of a threadlike structure revealed by Hemacolor staining. (f) Inner space (marked as “f” in (e)) within a vessel slice containing granules (about 1 μm, inset). Note that the inner space contains the resident cells of the threadlike structure.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4561983&req=5

fig7: The inner space structure containing cells along the inside of the subcutaneous threadlike structure. (a) Continuous inner space (arrows) along the longitudinal axis of a threadlike structure revealed by Hemacolor staining. (b) Distribution of the cells (large granular cells, arrowheads; granules, dotted circle) in the inner space (marked as “b” in (a), 20–30 μm) of the vessel. (c) Large inner space structure (> 50 μm) inside the subcutaneous threadlike structure showing low cellularity revealed by Hemacolor staining. (d) Distribution of the cells (small round cells, arrowheads) in the inner space (marked as “d” in (c)) of the vessel. (e) Cross-sectional image (200 μm) showing an inner space (dotted square, > 50 μm) of a threadlike structure revealed by Hemacolor staining. (f) Inner space (marked as “f” in (e)) within a vessel slice containing granules (about 1 μm, inset). Note that the inner space contains the resident cells of the threadlike structure.
Mentions: The inner regions of the vessel of the threadlike structure revealed by Hemacolor staining appeared as white space, indicating a lower cellularity (Figure 7). The inner space was linearly continuous along the longitudinal axis of the vessel (Figures 7(a) and 7(b)). The diameters of the inner space were 20–60 μm. The inner space structures contained various cells, such as large granular cells (Figure 7(b), arrowheads), granules (Figure 7(b), dotted circle), and small round cells (Figure 7(d), arrowheads). Figure 7(c) shows a bundle-like structure revealed by Hemacolor staining as shown in Figure 3 by scanning electron microscopy. In addition, a cross-sectioned slice (200 μm) of the threadlike structure was stained, and the small and large granular cells were observed in the whole threadlike structures. As shown in Figures 7(e) and 7(f), there was a sinus (~60 μm) that contained granules (~1 μm).

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.