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Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.


Hemacolor-stained threadlike structures in the rat abdominal subcutaneous tissue. (a) Typical example of the distribution of threadlike structures (white arrows) on the subcutaneous area stained by Hemacolor. ((b) and (c)) Threadlike structures (dotted squares marked as “b” and “c” in (a)) composed of the vessels (arrowheads) that connect the nodes (arrows). Note that the node structures are multibranched (white arrows) from the nodes, and the vessel structures are distributed either on or beneath the subcutaneous surface (two-way dotted white arrows and open arrowheads). Note that the blood vessels (BVs, asterisks) maintain their red color and that threadlike structures are aligned in either a vertical or horizontal direction (yellow arrows). Note the blood vessels around the branching points of the threadlike structures (asterisks).
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fig4: Hemacolor-stained threadlike structures in the rat abdominal subcutaneous tissue. (a) Typical example of the distribution of threadlike structures (white arrows) on the subcutaneous area stained by Hemacolor. ((b) and (c)) Threadlike structures (dotted squares marked as “b” and “c” in (a)) composed of the vessels (arrowheads) that connect the nodes (arrows). Note that the node structures are multibranched (white arrows) from the nodes, and the vessel structures are distributed either on or beneath the subcutaneous surface (two-way dotted white arrows and open arrowheads). Note that the blood vessels (BVs, asterisks) maintain their red color and that threadlike structures are aligned in either a vertical or horizontal direction (yellow arrows). Note the blood vessels around the branching points of the threadlike structures (asterisks).

Mentions: Figure 4 shows a representative example of a dark blue-colored threadlike structure revealed by Hemacolor staining in the abdominal subcutaneous area. The vessels were divided into a vertically elongated type (yellow vertical arrow) along the abdominal middle line and a horizontally elongated type (yellow horizontal arrow) branched from each node form (Figure 4(b)). As shown in Figures 4(b) and 4(c), the dark blue-colored multiple-branched threadlike structures (white arrows) were distributed across the surface of the subcutaneous tissue. In addition, some vessels were also distributed beneath the surface of the subcutaneous tissue (Figures 4(b) and 4(c), two-way dotted white arrows and open arrowheads). The dark blue threadlike structures appeared to be interrupted where the structures were buried underneath the surface of the subcutaneous tissue layer. In addition, blood vessels were observed around the threadlike structures (Figures 4(b) and 4(c), asterisks). The blood vessels were not stained dark blue like the threadlike structures by Hemacolor staining; rather, they maintained their original red colors. The average size of the nodes of the threadlike structures was 0.94 ± 0.14 mm (n = 12), and the average thickness of the vessels was 0.21 ± 0.05 mm (n = 15).


Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Hemacolor-stained threadlike structures in the rat abdominal subcutaneous tissue. (a) Typical example of the distribution of threadlike structures (white arrows) on the subcutaneous area stained by Hemacolor. ((b) and (c)) Threadlike structures (dotted squares marked as “b” and “c” in (a)) composed of the vessels (arrowheads) that connect the nodes (arrows). Note that the node structures are multibranched (white arrows) from the nodes, and the vessel structures are distributed either on or beneath the subcutaneous surface (two-way dotted white arrows and open arrowheads). Note that the blood vessels (BVs, asterisks) maintain their red color and that threadlike structures are aligned in either a vertical or horizontal direction (yellow arrows). Note the blood vessels around the branching points of the threadlike structures (asterisks).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Hemacolor-stained threadlike structures in the rat abdominal subcutaneous tissue. (a) Typical example of the distribution of threadlike structures (white arrows) on the subcutaneous area stained by Hemacolor. ((b) and (c)) Threadlike structures (dotted squares marked as “b” and “c” in (a)) composed of the vessels (arrowheads) that connect the nodes (arrows). Note that the node structures are multibranched (white arrows) from the nodes, and the vessel structures are distributed either on or beneath the subcutaneous surface (two-way dotted white arrows and open arrowheads). Note that the blood vessels (BVs, asterisks) maintain their red color and that threadlike structures are aligned in either a vertical or horizontal direction (yellow arrows). Note the blood vessels around the branching points of the threadlike structures (asterisks).
Mentions: Figure 4 shows a representative example of a dark blue-colored threadlike structure revealed by Hemacolor staining in the abdominal subcutaneous area. The vessels were divided into a vertically elongated type (yellow vertical arrow) along the abdominal middle line and a horizontally elongated type (yellow horizontal arrow) branched from each node form (Figure 4(b)). As shown in Figures 4(b) and 4(c), the dark blue-colored multiple-branched threadlike structures (white arrows) were distributed across the surface of the subcutaneous tissue. In addition, some vessels were also distributed beneath the surface of the subcutaneous tissue (Figures 4(b) and 4(c), two-way dotted white arrows and open arrowheads). The dark blue threadlike structures appeared to be interrupted where the structures were buried underneath the surface of the subcutaneous tissue layer. In addition, blood vessels were observed around the threadlike structures (Figures 4(b) and 4(c), asterisks). The blood vessels were not stained dark blue like the threadlike structures by Hemacolor staining; rather, they maintained their original red colors. The average size of the nodes of the threadlike structures was 0.94 ± 0.14 mm (n = 12), and the average thickness of the vessels was 0.21 ± 0.05 mm (n = 15).

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.