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Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.


Identification of intact PVS tissue on the surface of abdominal organs in rats using Hemacolor and trypan blue staining. (a) PVS tissue on the surface of the small intestine stained by Hemacolor. (b) Magnified view of the organ surface PVS structure (square in (a)) composed of multiple-PNs (arrows), a PV (arrowhead), and a subbranching point (dotted arrows). (c) PVS tissue on the surface of the large intestine stained by trypan blue. (d) Magnified view of the organ surface PVS structure (square in (c)) composed of two PNs (arrows) and a PV (arrowhead).
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fig1: Identification of intact PVS tissue on the surface of abdominal organs in rats using Hemacolor and trypan blue staining. (a) PVS tissue on the surface of the small intestine stained by Hemacolor. (b) Magnified view of the organ surface PVS structure (square in (a)) composed of multiple-PNs (arrows), a PV (arrowhead), and a subbranching point (dotted arrows). (c) PVS tissue on the surface of the large intestine stained by trypan blue. (d) Magnified view of the organ surface PVS structure (square in (c)) composed of two PNs (arrows) and a PV (arrowhead).

Mentions: Previously, it has been shown that Hemacolor staining is effective in characterizing the gross morphology of the osPVS in isolation [6]. In this study, Hemacolor dyes were directly applied to the PVS tissues on the surfaces of the abdominal organs to determine whether the osPVS could be stained in vivo as it is stained by trypan blue, which is a well-known dye that is used to identify PVS in situ [2]. The Hemacolor staining revealed that the PVS was attached to the surface of the abdominal organ. Figures 1(a) and 1(b) show representative osPVS tissue composed of primo-nodes (PNs) and primo-vessels (PVs) on the surface of the small intestines revealed by Hemacolor staining. The Hemacolor staining of the osPVS was comparable to the trypan blue staining (Figures 1(c) and 1(d)).


Identification of Primo-Vascular System in Abdominal Subcutaneous Tissue Layer of Rats.

Lim CJ, Lee SY, Ryu PD - Evid Based Complement Alternat Med (2015)

Identification of intact PVS tissue on the surface of abdominal organs in rats using Hemacolor and trypan blue staining. (a) PVS tissue on the surface of the small intestine stained by Hemacolor. (b) Magnified view of the organ surface PVS structure (square in (a)) composed of multiple-PNs (arrows), a PV (arrowhead), and a subbranching point (dotted arrows). (c) PVS tissue on the surface of the large intestine stained by trypan blue. (d) Magnified view of the organ surface PVS structure (square in (c)) composed of two PNs (arrows) and a PV (arrowhead).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4561983&req=5

fig1: Identification of intact PVS tissue on the surface of abdominal organs in rats using Hemacolor and trypan blue staining. (a) PVS tissue on the surface of the small intestine stained by Hemacolor. (b) Magnified view of the organ surface PVS structure (square in (a)) composed of multiple-PNs (arrows), a PV (arrowhead), and a subbranching point (dotted arrows). (c) PVS tissue on the surface of the large intestine stained by trypan blue. (d) Magnified view of the organ surface PVS structure (square in (c)) composed of two PNs (arrows) and a PV (arrowhead).
Mentions: Previously, it has been shown that Hemacolor staining is effective in characterizing the gross morphology of the osPVS in isolation [6]. In this study, Hemacolor dyes were directly applied to the PVS tissues on the surfaces of the abdominal organs to determine whether the osPVS could be stained in vivo as it is stained by trypan blue, which is a well-known dye that is used to identify PVS in situ [2]. The Hemacolor staining revealed that the PVS was attached to the surface of the abdominal organ. Figures 1(a) and 1(b) show representative osPVS tissue composed of primo-nodes (PNs) and primo-vessels (PVs) on the surface of the small intestines revealed by Hemacolor staining. The Hemacolor staining of the osPVS was comparable to the trypan blue staining (Figures 1(c) and 1(d)).

Bottom Line: The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints.In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians.Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.

ABSTRACT
The primo-vascular system (PVS) is a novel network identified in various animal tissues. However, the PVS in subcutaneous tissue has not been well identified. Here, we examined the putative PVS on the surface of abdominal subcutaneous tissue in rats. Hemacolor staining revealed dark blue threadlike structures consisting of nodes and vessels, which were frequently observed bundled with blood vessels. The structure was filled with various immune cells including mast cells and WBCs. In the structure, there were inner spaces (20-60 µm) with low cellularity. Electron microscopy revealed a bundle structure and typical cytology common with the well-established organ surface PVS, which were different from those of the lymphatic vessel. Among several subcutaneous (sc) PVS tissues identified on the rat abdominal space, the most outstanding was the scPVS aligned along the ventral midline. The distribution pattern of nodes and vessels in the scPVS closely resembled that of the conception vessel meridian and its acupoints. In conclusion, our results newly revealed that the PVS is present in the abdominal subcutaneous tissue layer and indicate that the scPVS tissues are closely correlated with acupuncture meridians. Our findings will help to characterize the PVS in the other superficial tissues and its physiological roles.

No MeSH data available.