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Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells.

Akhtar MJ, Alhadlaq HA, Alshamsan A, Majeed Khan MA, Ahamed M - Sci Rep (2015)

Bottom Line: Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm.The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml.Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway.

View Article: PubMed Central - PubMed

Affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.

ABSTRACT
We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selective cytotoxicity of ZnO nanoparticles against cancer cells can be further improved.

No MeSH data available.


Related in: MedlinePlus

Oxidative stress response of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) ROS, (B) GSH and (C) TSH. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and ROS.
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f5: Oxidative stress response of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) ROS, (B) GSH and (C) TSH. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and ROS.

Mentions: Both pure and Al-doped ZnO nanoparticles were found to induce oxidative stress indicated by induction of ROS generation and depletion of glutathione (GSH) and total antioxidant (TSH) (Fig. 5A–C). Moreover, oxidative stress response of Al-doped ZnO nanoparticles was more severe than those of pure one. We also observed an inverse correlation between ROS and cell viability (Fig. 5D).


Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells.

Akhtar MJ, Alhadlaq HA, Alshamsan A, Majeed Khan MA, Ahamed M - Sci Rep (2015)

Oxidative stress response of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) ROS, (B) GSH and (C) TSH. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and ROS.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561961&req=5

f5: Oxidative stress response of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) ROS, (B) GSH and (C) TSH. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and ROS.
Mentions: Both pure and Al-doped ZnO nanoparticles were found to induce oxidative stress indicated by induction of ROS generation and depletion of glutathione (GSH) and total antioxidant (TSH) (Fig. 5A–C). Moreover, oxidative stress response of Al-doped ZnO nanoparticles was more severe than those of pure one. We also observed an inverse correlation between ROS and cell viability (Fig. 5D).

Bottom Line: Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm.The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml.Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway.

View Article: PubMed Central - PubMed

Affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.

ABSTRACT
We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selective cytotoxicity of ZnO nanoparticles against cancer cells can be further improved.

No MeSH data available.


Related in: MedlinePlus