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Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells.

Akhtar MJ, Alhadlaq HA, Alshamsan A, Majeed Khan MA, Ahamed M - Sci Rep (2015)

Bottom Line: Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm.The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml.Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway.

View Article: PubMed Central - PubMed

Affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.

ABSTRACT
We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selective cytotoxicity of ZnO nanoparticles against cancer cells can be further improved.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) MTT assay (B) NRU assay and (C) LDH assay. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and LDH.
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f4: Cytotoxicity of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) MTT assay (B) NRU assay and (C) LDH assay. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and LDH.

Mentions: MCF-7 cells were exposed to pure and Al-doped ZnO nanoparticles at the concentrations of 25, 50 and 100 μg/ml for 24 h and cytotoxicity was determined by MTT, NRU and LDH assays. All three assays have shown that cytotoxic response of Al-doped ZnO nanoparticles was higher as compared to pure ZnO nanoparticles. MTT cell viability due to pure ZnO nanoparticles exposure was decreased to 80%, 46% and 31%, while cell viability reduction due to Al-doped ZnO nanoparticles was 67%, 33% and 22% at the concentrations of 25, 50 100 mg/ml, respectively (Fig. 4A). The IC50 estimated by MTT assay was 44 μg/ml for pure ZnO nanoparticles and 31 μg/ml for Al-doped ZnO nanoparticles. Similar trends of cell viability were observed in NRU results (Fig. 4B).


Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells.

Akhtar MJ, Alhadlaq HA, Alshamsan A, Majeed Khan MA, Ahamed M - Sci Rep (2015)

Cytotoxicity of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) MTT assay (B) NRU assay and (C) LDH assay. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and LDH.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561961&req=5

f4: Cytotoxicity of pure and Al-doped ZnO nanoparticles in MCF-7 cells.(A) MTT assay (B) NRU assay and (C) LDH assay. Data represented are mean ± SD of three identical experiments made in triplicate. *Statistically significant difference as compared to controls (p < 0.05). (D) Significant negative correlation between MTT and LDH.
Mentions: MCF-7 cells were exposed to pure and Al-doped ZnO nanoparticles at the concentrations of 25, 50 and 100 μg/ml for 24 h and cytotoxicity was determined by MTT, NRU and LDH assays. All three assays have shown that cytotoxic response of Al-doped ZnO nanoparticles was higher as compared to pure ZnO nanoparticles. MTT cell viability due to pure ZnO nanoparticles exposure was decreased to 80%, 46% and 31%, while cell viability reduction due to Al-doped ZnO nanoparticles was 67%, 33% and 22% at the concentrations of 25, 50 100 mg/ml, respectively (Fig. 4A). The IC50 estimated by MTT assay was 44 μg/ml for pure ZnO nanoparticles and 31 μg/ml for Al-doped ZnO nanoparticles. Similar trends of cell viability were observed in NRU results (Fig. 4B).

Bottom Line: Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm.The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml.Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway.

View Article: PubMed Central - PubMed

Affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.

ABSTRACT
We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selective cytotoxicity of ZnO nanoparticles against cancer cells can be further improved.

No MeSH data available.


Related in: MedlinePlus