Limits...
Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells.

Akhtar MJ, Alhadlaq HA, Alshamsan A, Majeed Khan MA, Ahamed M - Sci Rep (2015)

Bottom Line: Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm.The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml.Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway.

View Article: PubMed Central - PubMed

Affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.

ABSTRACT
We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selective cytotoxicity of ZnO nanoparticles against cancer cells can be further improved.

No MeSH data available.


Related in: MedlinePlus

TEM images of pure (A) and Al-doped (B) ZnO nanoparticles. Inset shows HR-TEM of same samples. EDS analysis of pure (C) and Al-doped (D) ZnO nanoparticles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4561961&req=5

f2: TEM images of pure (A) and Al-doped (B) ZnO nanoparticles. Inset shows HR-TEM of same samples. EDS analysis of pure (C) and Al-doped (D) ZnO nanoparticles.

Mentions: Morphology of pure and Al-doped ZnO nanoparticles was investigated by FETEM. Figure 2A,B represent the micrographs of pure and Al-doped ZnO nanoparticles, respectively. These images demonstrated that grown nanoparticles were almost spherical shaped with the particle size range of 33–55 nm. High resolution TEM images represented at insets showing that particles nature were crystalline that supports XRD data. TEM average diameter was calculated from measuring over 100 particles in random fields of view. The average particle size of pure and Al-doped ZnO nanoparticles were approximately 43 and 40 nm, respectively. The average particle size obtained from TEM matches well with the size estimated from the XRD. Both TEM and XRD studies showed a slight decrease in ZnO nanoparticles size with Al-doping. This is a common trend when a dopant is incorporated into ZnO particles and supported by other studies1123. The size reduction of Al-doped ZnO samples may be owing to smaller ionic radius of Al compared to ionic radius of Zn.


Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells.

Akhtar MJ, Alhadlaq HA, Alshamsan A, Majeed Khan MA, Ahamed M - Sci Rep (2015)

TEM images of pure (A) and Al-doped (B) ZnO nanoparticles. Inset shows HR-TEM of same samples. EDS analysis of pure (C) and Al-doped (D) ZnO nanoparticles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561961&req=5

f2: TEM images of pure (A) and Al-doped (B) ZnO nanoparticles. Inset shows HR-TEM of same samples. EDS analysis of pure (C) and Al-doped (D) ZnO nanoparticles.
Mentions: Morphology of pure and Al-doped ZnO nanoparticles was investigated by FETEM. Figure 2A,B represent the micrographs of pure and Al-doped ZnO nanoparticles, respectively. These images demonstrated that grown nanoparticles were almost spherical shaped with the particle size range of 33–55 nm. High resolution TEM images represented at insets showing that particles nature were crystalline that supports XRD data. TEM average diameter was calculated from measuring over 100 particles in random fields of view. The average particle size of pure and Al-doped ZnO nanoparticles were approximately 43 and 40 nm, respectively. The average particle size obtained from TEM matches well with the size estimated from the XRD. Both TEM and XRD studies showed a slight decrease in ZnO nanoparticles size with Al-doping. This is a common trend when a dopant is incorporated into ZnO particles and supported by other studies1123. The size reduction of Al-doped ZnO samples may be owing to smaller ionic radius of Al compared to ionic radius of Zn.

Bottom Line: Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm.The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml.Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway.

View Article: PubMed Central - PubMed

Affiliation: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.

ABSTRACT
We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selective cytotoxicity of ZnO nanoparticles against cancer cells can be further improved.

No MeSH data available.


Related in: MedlinePlus