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Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.


A miRNA-transcripts model for Expression Regulation during hybridization.(A) Genome level. Duplication (indicated by red triangles) and deletion (indicated by green bars) of loci in genome during hybridization. (B) miRNA regulation level. Nonadditively expressed miRNAs target genes involved in response to immune system and development. (C) Expression levels of protein-coding genes. ELD genes and nonadditively expressed genes contribute to the heterosis in newly formed hybrids.
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f7: A miRNA-transcripts model for Expression Regulation during hybridization.(A) Genome level. Duplication (indicated by red triangles) and deletion (indicated by green bars) of loci in genome during hybridization. (B) miRNA regulation level. Nonadditively expressed miRNAs target genes involved in response to immune system and development. (C) Expression levels of protein-coding genes. ELD genes and nonadditively expressed genes contribute to the heterosis in newly formed hybrids.

Mentions: The new hybrid M. amblycephala × C. alburnus lines, from different genera with the B and T genomes, indicated a genetic melting of organisms, exhibiting improved adaption to stress. Meanwhile, this heterosis was inheritable from F1 to F2, which guarantees a stable appearance in progenies. These ameliorative and hereditable phenotypes result from genome variation, and cause miRNA and mRNA expression changes; these findings propose a gene regulatory scheme for hybrids. We showed a dynamic regulation of homoeologs in newly formed hybrids, and demonstrated the miRNA regulated gene expression in the melting genomes (Fig. 7). We propose three different levels for heterosis that includes improved stress and adaptability during hybridization. First, the interaction between subgenomes from different species leads to gene loss or duplication as well as DNA modification such as methylation in the newly formed genome. Second, nonadditively expressed miRNAs affect target genes that in turn yield the improved stress and development. Third, for protein-coding genes, the expression of ELD-B genes may contribute to cell development, while that of ELD-T genes are involved in stress and improved adaptability. Taken together, the expression changes of miRNA and mRNA in hybrids showed stable variation during the early hybridization, and were passed down from generation to generation, which accounts for the successful advent of interspecies fishes, providing evolutionary force in natural population.


Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

A miRNA-transcripts model for Expression Regulation during hybridization.(A) Genome level. Duplication (indicated by red triangles) and deletion (indicated by green bars) of loci in genome during hybridization. (B) miRNA regulation level. Nonadditively expressed miRNAs target genes involved in response to immune system and development. (C) Expression levels of protein-coding genes. ELD genes and nonadditively expressed genes contribute to the heterosis in newly formed hybrids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561955&req=5

f7: A miRNA-transcripts model for Expression Regulation during hybridization.(A) Genome level. Duplication (indicated by red triangles) and deletion (indicated by green bars) of loci in genome during hybridization. (B) miRNA regulation level. Nonadditively expressed miRNAs target genes involved in response to immune system and development. (C) Expression levels of protein-coding genes. ELD genes and nonadditively expressed genes contribute to the heterosis in newly formed hybrids.
Mentions: The new hybrid M. amblycephala × C. alburnus lines, from different genera with the B and T genomes, indicated a genetic melting of organisms, exhibiting improved adaption to stress. Meanwhile, this heterosis was inheritable from F1 to F2, which guarantees a stable appearance in progenies. These ameliorative and hereditable phenotypes result from genome variation, and cause miRNA and mRNA expression changes; these findings propose a gene regulatory scheme for hybrids. We showed a dynamic regulation of homoeologs in newly formed hybrids, and demonstrated the miRNA regulated gene expression in the melting genomes (Fig. 7). We propose three different levels for heterosis that includes improved stress and adaptability during hybridization. First, the interaction between subgenomes from different species leads to gene loss or duplication as well as DNA modification such as methylation in the newly formed genome. Second, nonadditively expressed miRNAs affect target genes that in turn yield the improved stress and development. Third, for protein-coding genes, the expression of ELD-B genes may contribute to cell development, while that of ELD-T genes are involved in stress and improved adaptability. Taken together, the expression changes of miRNA and mRNA in hybrids showed stable variation during the early hybridization, and were passed down from generation to generation, which accounts for the successful advent of interspecies fishes, providing evolutionary force in natural population.

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.