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Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.


Effect of hybridization on expression of miRNAs.BB, M. amblycephala, TT, C. alburnus. (A) Nonadditive expression and miRNAs with parental ELD in F1 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure). (B) Nonadditive expression and miRNAs with parental ELD in F2 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure for concise). (C) Negative correlations between log2 fold changes of a subset of differentially expressed miRNAs and those of differentially expressed targets. Multiple genes are targeted by one miRNA which were indicated by vertical line. r, Pearson correlation efficient.
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f6: Effect of hybridization on expression of miRNAs.BB, M. amblycephala, TT, C. alburnus. (A) Nonadditive expression and miRNAs with parental ELD in F1 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure). (B) Nonadditive expression and miRNAs with parental ELD in F2 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure for concise). (C) Negative correlations between log2 fold changes of a subset of differentially expressed miRNAs and those of differentially expressed targets. Multiple genes are targeted by one miRNA which were indicated by vertical line. r, Pearson correlation efficient.

Mentions: Similar with protein-coding genes, some miRNA also showed parental ELD in the newly formed hybrids. In F1, ELD-B (II + XI, 16), which was significantly less than those miRNAs showed ELD-T (IV + IX, 33) (Fig. 6A; Supplementary Table 3 and Supplementary Dataset 4). Similar results were obtained in F2 (ELD-B, 14 versus ELD-T, 20) (Fig. 6B; Supplementary Table 3 and Supplementary Dataset 4). Only 14 ELD miRNAs were inherited from F1 to F2, including several immune related and development regulatory miRNAs. Interestingly, miR-133a-3p29, miR-459-3p30 and miR-73331, which were reported for immune regulation, showed ELD.


Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Effect of hybridization on expression of miRNAs.BB, M. amblycephala, TT, C. alburnus. (A) Nonadditive expression and miRNAs with parental ELD in F1 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure). (B) Nonadditive expression and miRNAs with parental ELD in F2 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure for concise). (C) Negative correlations between log2 fold changes of a subset of differentially expressed miRNAs and those of differentially expressed targets. Multiple genes are targeted by one miRNA which were indicated by vertical line. r, Pearson correlation efficient.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561955&req=5

f6: Effect of hybridization on expression of miRNAs.BB, M. amblycephala, TT, C. alburnus. (A) Nonadditive expression and miRNAs with parental ELD in F1 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure). (B) Nonadditive expression and miRNAs with parental ELD in F2 (only miRNAs which both attributed to nonadditive and parental ELD were showed in this Figure for concise). (C) Negative correlations between log2 fold changes of a subset of differentially expressed miRNAs and those of differentially expressed targets. Multiple genes are targeted by one miRNA which were indicated by vertical line. r, Pearson correlation efficient.
Mentions: Similar with protein-coding genes, some miRNA also showed parental ELD in the newly formed hybrids. In F1, ELD-B (II + XI, 16), which was significantly less than those miRNAs showed ELD-T (IV + IX, 33) (Fig. 6A; Supplementary Table 3 and Supplementary Dataset 4). Similar results were obtained in F2 (ELD-B, 14 versus ELD-T, 20) (Fig. 6B; Supplementary Table 3 and Supplementary Dataset 4). Only 14 ELD miRNAs were inherited from F1 to F2, including several immune related and development regulatory miRNAs. Interestingly, miR-133a-3p29, miR-459-3p30 and miR-73331, which were reported for immune regulation, showed ELD.

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.