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Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.


ELD of Genes in M. amblycephala × C. alburnus hybrids. (A) Twelve bins of differentially expressed genes. B, M. amblycephala, T, C. alburnus. H, hybrids. (B) Enriched GO terms of genes showing parental ELD in F1 and F2. *P < 0.05 and **P < 0.01 to indicate the significant difference.
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f4: ELD of Genes in M. amblycephala × C. alburnus hybrids. (A) Twelve bins of differentially expressed genes. B, M. amblycephala, T, C. alburnus. H, hybrids. (B) Enriched GO terms of genes showing parental ELD in F1 and F2. *P < 0.05 and **P < 0.01 to indicate the significant difference.

Mentions: In hybrids, ELD genes are those for which the expression level is statistically similar to one parent while different from the other. According to the previously described classification, we divided genes into 12 categories (as shown in Fig. 4A), based on differential expression patterns by comparing the hybrids to their parents as used in Rapp et al. (2009)21. We classified the ELD genes in F1 and F2 that showed similar expression with M. amblycephala as ELD-B genes; those with equivalent expression with C. alburnus were labeled ELD-T genes. In F1 and F2, 1387 and 665 genes showed parental ELD, respectively (Fig. 4A). In addition, both F1 and F2 showed no significant difference of gene number between ELD-B and ELD-T genes. Namely, these genes in the newly formed hybrids had no ELD bias toward the parents.


Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

ELD of Genes in M. amblycephala × C. alburnus hybrids. (A) Twelve bins of differentially expressed genes. B, M. amblycephala, T, C. alburnus. H, hybrids. (B) Enriched GO terms of genes showing parental ELD in F1 and F2. *P < 0.05 and **P < 0.01 to indicate the significant difference.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561955&req=5

f4: ELD of Genes in M. amblycephala × C. alburnus hybrids. (A) Twelve bins of differentially expressed genes. B, M. amblycephala, T, C. alburnus. H, hybrids. (B) Enriched GO terms of genes showing parental ELD in F1 and F2. *P < 0.05 and **P < 0.01 to indicate the significant difference.
Mentions: In hybrids, ELD genes are those for which the expression level is statistically similar to one parent while different from the other. According to the previously described classification, we divided genes into 12 categories (as shown in Fig. 4A), based on differential expression patterns by comparing the hybrids to their parents as used in Rapp et al. (2009)21. We classified the ELD genes in F1 and F2 that showed similar expression with M. amblycephala as ELD-B genes; those with equivalent expression with C. alburnus were labeled ELD-T genes. In F1 and F2, 1387 and 665 genes showed parental ELD, respectively (Fig. 4A). In addition, both F1 and F2 showed no significant difference of gene number between ELD-B and ELD-T genes. Namely, these genes in the newly formed hybrids had no ELD bias toward the parents.

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.