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Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.


Nonadditively Expressed Genes in F1 and F2.BB, M. amblycephala, TT, C. alburnus. (A) Genes differentially expressed in F1 and their progenitors. (B) Genes differentially expressed in F2 and their progenitors. Numbers close to the species represent upregulated genes compared with the neighboring species. The number showed in bold indicates the total number of genes differentially expressed between two species.
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f3: Nonadditively Expressed Genes in F1 and F2.BB, M. amblycephala, TT, C. alburnus. (A) Genes differentially expressed in F1 and their progenitors. (B) Genes differentially expressed in F2 and their progenitors. Numbers close to the species represent upregulated genes compared with the neighboring species. The number showed in bold indicates the total number of genes differentially expressed between two species.

Mentions: Among all the expressed genes, 3712 (66.69%) were differentially expressed between M. amblycephala and C. alburnus (Fig. 3A,B). For the 3712 genes differentially expressed between the progenitors, 1758 (31.58%) showed higher expression levels in M. amblycephala (BB > TT), whereas 1954 (35.11%) were expressed at higher levels in C. alburnus (BB < TT). Compared with the progenitors, F2 showed higher number of differentially expressed genes than F1. For F1, 4441 (79.79%) F1-BBdif genes were observed which were more than that of F1-TTdif genes (4382, 78.73%) (P < 0.05, BH multiple test correction) (Fig. 3A and Supplementary Table 2). For F2, the numbers of F2-BBdif (5030, 90.37%) and F2-TTdif (5020, 90.19%) genes were almost equal (P > 0.05, BH multiple test correction) (Fig. 3B and Supplementary Table 2).


Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Nonadditively Expressed Genes in F1 and F2.BB, M. amblycephala, TT, C. alburnus. (A) Genes differentially expressed in F1 and their progenitors. (B) Genes differentially expressed in F2 and their progenitors. Numbers close to the species represent upregulated genes compared with the neighboring species. The number showed in bold indicates the total number of genes differentially expressed between two species.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561955&req=5

f3: Nonadditively Expressed Genes in F1 and F2.BB, M. amblycephala, TT, C. alburnus. (A) Genes differentially expressed in F1 and their progenitors. (B) Genes differentially expressed in F2 and their progenitors. Numbers close to the species represent upregulated genes compared with the neighboring species. The number showed in bold indicates the total number of genes differentially expressed between two species.
Mentions: Among all the expressed genes, 3712 (66.69%) were differentially expressed between M. amblycephala and C. alburnus (Fig. 3A,B). For the 3712 genes differentially expressed between the progenitors, 1758 (31.58%) showed higher expression levels in M. amblycephala (BB > TT), whereas 1954 (35.11%) were expressed at higher levels in C. alburnus (BB < TT). Compared with the progenitors, F2 showed higher number of differentially expressed genes than F1. For F1, 4441 (79.79%) F1-BBdif genes were observed which were more than that of F1-TTdif genes (4382, 78.73%) (P < 0.05, BH multiple test correction) (Fig. 3A and Supplementary Table 2). For F2, the numbers of F2-BBdif (5030, 90.37%) and F2-TTdif (5020, 90.19%) genes were almost equal (P > 0.05, BH multiple test correction) (Fig. 3B and Supplementary Table 2).

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.