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Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.


Formation of M. amblycephala × C. alburnus hybrids. (A) 48 Chromosomes were observed in M. amblycephala. (B) 48 Chromosomes were observed in C. alburnus. (C,D) After hybridization, F1 (C) and F2 (D) were obtained. The observation of chromosomes showed that no duplication of genome could found. The histology of gonads in hybrid showed fertile of these fishes. The photographs of the fishes were taken by Jun Xiao.
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f1: Formation of M. amblycephala × C. alburnus hybrids. (A) 48 Chromosomes were observed in M. amblycephala. (B) 48 Chromosomes were observed in C. alburnus. (C,D) After hybridization, F1 (C) and F2 (D) were obtained. The observation of chromosomes showed that no duplication of genome could found. The histology of gonads in hybrid showed fertile of these fishes. The photographs of the fishes were taken by Jun Xiao.

Mentions: Hybrid fish were generated from female M. amblycephala × male C. alburnus (Fig. 1A,B). Two types of F1 hybrids were obtained in the present study, including diploid (2n = 48) and triploid (3n = 72) hybrids as previously reported19. In order to remove the polyploidy variable, we only used diploid hybrids (F1) for subsequent evaluation (Fig. 1C). Histology of gonads was performed and both mature oocytes and sperms were observed in F1 (Fig. 1C). Subsequently, diploid F2 individuals were obtained by self-mating of F1 (Fig. 1D). The diploid hybrids were bisexually fertile with normal development of gametes (Fig. 1D). Meanwhile, as previously reported19, for both diploid F1 and F2, 48 chromosomes (24 from M. amblycephala and 24 from C. alburnus) with no polyploidization, are suitable for further experimentation.


Global transcriptional and miRNA insights into bases of heterosis in hybridization of Cyprinidae.

Zhou Y, Ren L, Xiao J, Zhong H, Wang J, Hu J, Yu F, Tao M, Zhang C, Liu Y, Liu S - Sci Rep (2015)

Formation of M. amblycephala × C. alburnus hybrids. (A) 48 Chromosomes were observed in M. amblycephala. (B) 48 Chromosomes were observed in C. alburnus. (C,D) After hybridization, F1 (C) and F2 (D) were obtained. The observation of chromosomes showed that no duplication of genome could found. The histology of gonads in hybrid showed fertile of these fishes. The photographs of the fishes were taken by Jun Xiao.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561955&req=5

f1: Formation of M. amblycephala × C. alburnus hybrids. (A) 48 Chromosomes were observed in M. amblycephala. (B) 48 Chromosomes were observed in C. alburnus. (C,D) After hybridization, F1 (C) and F2 (D) were obtained. The observation of chromosomes showed that no duplication of genome could found. The histology of gonads in hybrid showed fertile of these fishes. The photographs of the fishes were taken by Jun Xiao.
Mentions: Hybrid fish were generated from female M. amblycephala × male C. alburnus (Fig. 1A,B). Two types of F1 hybrids were obtained in the present study, including diploid (2n = 48) and triploid (3n = 72) hybrids as previously reported19. In order to remove the polyploidy variable, we only used diploid hybrids (F1) for subsequent evaluation (Fig. 1C). Histology of gonads was performed and both mature oocytes and sperms were observed in F1 (Fig. 1C). Subsequently, diploid F2 individuals were obtained by self-mating of F1 (Fig. 1D). The diploid hybrids were bisexually fertile with normal development of gametes (Fig. 1D). Meanwhile, as previously reported19, for both diploid F1 and F2, 48 chromosomes (24 from M. amblycephala and 24 from C. alburnus) with no polyploidization, are suitable for further experimentation.

Bottom Line: Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies.The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes.A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.

ABSTRACT
Hybrid Megalobrama amblycephala × Culter alburnus represents a population newly formed by interspecific crossing between two different genera. Here we assessed the expression pattern of mRNA and small RNA in newly formed F1, F2 and their progenitors. Large amounts of nonadditively expressed protein-coding genes showed parental expression level dominance (ELD). Interestingly, the ELD pattern could inherit from F1 to F2, which guaranteed a stable appearance in progenies. The ELD-B genes were found to contribute to cell development, while the ELD-T genes were enriched in function of stress and adaptability. microRNAs (miRNA) also had similar expression patterns to genes. A high proportion of miRNAs showed nonadditive expression upon hybridization, and were found to target important genes with diverse roles potentially involved in stress adaption and development. Taken together, the gene and miRNA expression divergence contributes to heterosis in the newly formed hybrid, promising the successful existence of hybrid speciation.

No MeSH data available.