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Increased frequency of circulating Tc22/Th22 cells and polyfunctional CD38(-) T cells in HIV-exposed uninfected subjects.

Oliveira LM, Lima JF, Cervantes CA, Casseb JS, Mendonça M, Duarte AJ, Sato MN - Sci Rep (2015)

Bottom Line: EUs exhibited an increased frequency of p15 Gag CD4+ IL-22+ secreting T cells, whereas HIV-infected partners demonstrated a high frequency of CD4+ IL-17+ T cells in response to p24.EUs demonstrated the presence of Tc22/Th22 cells and polyfunctional CD38- T cells with a low activation profile.These data suggest that SEB-induced polyfunctional CD4+ and CD8+ T cells together with Tc22/Th22 cells in EU individuals can provide an immunological advantage in the response to pathogens such as HIV-1.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Tropical Medicine Institute of São Paulo, University of São Paulo, São Paulo, Brazil.

ABSTRACT
Some individuals are resistant to HIV-1 infection despite repeated exposure to the virus, suggesting the presence of a complex antiviral response. Innate factors like IL-22 exert gut mucosal protection and polyfunctional T cells have been associated with low progression in HIV infection; therefore, we evaluated the frequencies of CD4+ and CD8+ T cell-secreting cytokines, including Tc22/Th22 cells and polyfunctional T cells in HIV-1-exposed uninfected individuals (EUs), their HIV-1-infected partners and healthy controls. EUs exhibited an increased frequency of p15 Gag CD4+ IL-22+ secreting T cells, whereas HIV-infected partners demonstrated a high frequency of CD4+ IL-17+ T cells in response to p24. Similar responses of Th22 and Tc22 cells to Gag peptides and Staphylococcal enterotoxin B (SEB) stimulation were detected in the serodiscordant couples. However, polyfunctionality in HIV subjects was associated with an HIV Gag response of CD38+ T cells, whereas polyfunctionality for EUs was induced upon SEB stimulation by CD38- T cells. EUs demonstrated the presence of Tc22/Th22 cells and polyfunctional CD38- T cells with a low activation profile. These data suggest that SEB-induced polyfunctional CD4+ and CD8+ T cells together with Tc22/Th22 cells in EU individuals can provide an immunological advantage in the response to pathogens such as HIV-1.

No MeSH data available.


Related in: MedlinePlus

SEB-induced polyfunctional CD4+ and CD8+ T cells as features of EUs.The simultaneous secretion of IFN-γ, IL-2, IL-17a, IL-22 and MIP-1β by CD4+ (A) and CD8+ (B) T cells induced by Gag1 (p17), Gag2 (p24), or SEB from HC (n = 15), EU (n = 16) and HIV (n = 15) individuals was assessed using flow cytometry. Frequencies of CD4+ and CD8+ T cells were subtracted from baseline values. Bars represent the medians and IQRs. *p ≤ 0.05 and **p ≤ 0.01, adjusted for multiple comparisons.
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f3: SEB-induced polyfunctional CD4+ and CD8+ T cells as features of EUs.The simultaneous secretion of IFN-γ, IL-2, IL-17a, IL-22 and MIP-1β by CD4+ (A) and CD8+ (B) T cells induced by Gag1 (p17), Gag2 (p24), or SEB from HC (n = 15), EU (n = 16) and HIV (n = 15) individuals was assessed using flow cytometry. Frequencies of CD4+ and CD8+ T cells were subtracted from baseline values. Bars represent the medians and IQRs. *p ≤ 0.05 and **p ≤ 0.01, adjusted for multiple comparisons.

Mentions: A polyfunctional CD4+ T cell response to Gag stimulation was detected only in the HIV-infected group (Fig. 3). Increased frequency of CD4+ T cells secreting 5 cytokines were detected in response to Gag2 stimulation, or 4 cytokines in combination induced by Gag2 (excluding IL-22 or IL-17) and Gag1 (excluding IL-2) stimulation (Fig. 3A). In contrast, EU subjects demonstrated a polyfunctional response compared to the HIV-infected group only in response to SEB stimulation, for example, the secretion of 4 cytokines (IFN-γ, IL-2, IL-17a, and IL-22) or a combination of 3 cytokines (including IFN-γ, IL-2 and IL-22).


Increased frequency of circulating Tc22/Th22 cells and polyfunctional CD38(-) T cells in HIV-exposed uninfected subjects.

Oliveira LM, Lima JF, Cervantes CA, Casseb JS, Mendonça M, Duarte AJ, Sato MN - Sci Rep (2015)

SEB-induced polyfunctional CD4+ and CD8+ T cells as features of EUs.The simultaneous secretion of IFN-γ, IL-2, IL-17a, IL-22 and MIP-1β by CD4+ (A) and CD8+ (B) T cells induced by Gag1 (p17), Gag2 (p24), or SEB from HC (n = 15), EU (n = 16) and HIV (n = 15) individuals was assessed using flow cytometry. Frequencies of CD4+ and CD8+ T cells were subtracted from baseline values. Bars represent the medians and IQRs. *p ≤ 0.05 and **p ≤ 0.01, adjusted for multiple comparisons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561954&req=5

f3: SEB-induced polyfunctional CD4+ and CD8+ T cells as features of EUs.The simultaneous secretion of IFN-γ, IL-2, IL-17a, IL-22 and MIP-1β by CD4+ (A) and CD8+ (B) T cells induced by Gag1 (p17), Gag2 (p24), or SEB from HC (n = 15), EU (n = 16) and HIV (n = 15) individuals was assessed using flow cytometry. Frequencies of CD4+ and CD8+ T cells were subtracted from baseline values. Bars represent the medians and IQRs. *p ≤ 0.05 and **p ≤ 0.01, adjusted for multiple comparisons.
Mentions: A polyfunctional CD4+ T cell response to Gag stimulation was detected only in the HIV-infected group (Fig. 3). Increased frequency of CD4+ T cells secreting 5 cytokines were detected in response to Gag2 stimulation, or 4 cytokines in combination induced by Gag2 (excluding IL-22 or IL-17) and Gag1 (excluding IL-2) stimulation (Fig. 3A). In contrast, EU subjects demonstrated a polyfunctional response compared to the HIV-infected group only in response to SEB stimulation, for example, the secretion of 4 cytokines (IFN-γ, IL-2, IL-17a, and IL-22) or a combination of 3 cytokines (including IFN-γ, IL-2 and IL-22).

Bottom Line: EUs exhibited an increased frequency of p15 Gag CD4+ IL-22+ secreting T cells, whereas HIV-infected partners demonstrated a high frequency of CD4+ IL-17+ T cells in response to p24.EUs demonstrated the presence of Tc22/Th22 cells and polyfunctional CD38- T cells with a low activation profile.These data suggest that SEB-induced polyfunctional CD4+ and CD8+ T cells together with Tc22/Th22 cells in EU individuals can provide an immunological advantage in the response to pathogens such as HIV-1.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Tropical Medicine Institute of São Paulo, University of São Paulo, São Paulo, Brazil.

ABSTRACT
Some individuals are resistant to HIV-1 infection despite repeated exposure to the virus, suggesting the presence of a complex antiviral response. Innate factors like IL-22 exert gut mucosal protection and polyfunctional T cells have been associated with low progression in HIV infection; therefore, we evaluated the frequencies of CD4+ and CD8+ T cell-secreting cytokines, including Tc22/Th22 cells and polyfunctional T cells in HIV-1-exposed uninfected individuals (EUs), their HIV-1-infected partners and healthy controls. EUs exhibited an increased frequency of p15 Gag CD4+ IL-22+ secreting T cells, whereas HIV-infected partners demonstrated a high frequency of CD4+ IL-17+ T cells in response to p24. Similar responses of Th22 and Tc22 cells to Gag peptides and Staphylococcal enterotoxin B (SEB) stimulation were detected in the serodiscordant couples. However, polyfunctionality in HIV subjects was associated with an HIV Gag response of CD38+ T cells, whereas polyfunctionality for EUs was induced upon SEB stimulation by CD38- T cells. EUs demonstrated the presence of Tc22/Th22 cells and polyfunctional CD38- T cells with a low activation profile. These data suggest that SEB-induced polyfunctional CD4+ and CD8+ T cells together with Tc22/Th22 cells in EU individuals can provide an immunological advantage in the response to pathogens such as HIV-1.

No MeSH data available.


Related in: MedlinePlus