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Ecology and Distribution of Thaumarchaea in the Deep Hypolimnion of Lake Maggiore.

Coci M, Odermatt N, Salcher MM, Pernthaler J, Corno G - Archaea (2015)

Bottom Line: In order to reach a high resolution at the Thaumarchaea community level, the probe MGI-535 was specifically designed for this study and applied to fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) analysis.We then applied it to a fine analysis of diversity and relative abundance of AOA in the deepest layers of the oligotrophic Lake Maggiore, confirming previous published results of AOA presence, but showing differences in abundance and distribution within the water column without significant seasonal trends with respect to Bacteria.Furthermore, phylogenetic analysis of AOA clone libraries from deep lake water and from a lake tributary, River Maggia, suggested the riverine origin of AOA of the deep hypolimnion of the lake.

View Article: PubMed Central - PubMed

Affiliation: Microbial Ecology Group, CNR-Institute of Ecosystem Study, Largo Tonolli 50, 28922 Verbania, Italy ; Microb&Co, Association for Microbial Ecology, Viale XX Settembre 45, 95128 Catania, Italy.

ABSTRACT
Ammonia-oxidizing Archaea (AOA) play an important role in the oxidation of ammonia in terrestrial, marine, and geothermal habitats, as confirmed by a number of studies specifically focused on those environments. Much less is known about the ecological role of AOA in freshwaters. In order to reach a high resolution at the Thaumarchaea community level, the probe MGI-535 was specifically designed for this study and applied to fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) analysis. We then applied it to a fine analysis of diversity and relative abundance of AOA in the deepest layers of the oligotrophic Lake Maggiore, confirming previous published results of AOA presence, but showing differences in abundance and distribution within the water column without significant seasonal trends with respect to Bacteria. Furthermore, phylogenetic analysis of AOA clone libraries from deep lake water and from a lake tributary, River Maggia, suggested the riverine origin of AOA of the deep hypolimnion of the lake.

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Total prokaryotic abundance (±s.d.) at 3, 10, 200, and 350 m depth at Ghiffa pelagic station, Lake Maggiore, in 2011 obtained from counts of DAPI stained cells.
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fig2: Total prokaryotic abundance (±s.d.) at 3, 10, 200, and 350 m depth at Ghiffa pelagic station, Lake Maggiore, in 2011 obtained from counts of DAPI stained cells.

Mentions: Total abundance of prokaryotic cells (Bacteria and Archaea) displayed seasonal maxima of up to 7.3 × 106 cells mL−1 at 3 and 10 m depth concomitant with the spring (March-April) and summer (July) phytoplankton blooms. No seasonal trend was visible in the deep water layers (200 m and 350 m depth) where the overall abundance was generally lower than 1 × 106 cells mL−1 (Figure 2).


Ecology and Distribution of Thaumarchaea in the Deep Hypolimnion of Lake Maggiore.

Coci M, Odermatt N, Salcher MM, Pernthaler J, Corno G - Archaea (2015)

Total prokaryotic abundance (±s.d.) at 3, 10, 200, and 350 m depth at Ghiffa pelagic station, Lake Maggiore, in 2011 obtained from counts of DAPI stained cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4561949&req=5

fig2: Total prokaryotic abundance (±s.d.) at 3, 10, 200, and 350 m depth at Ghiffa pelagic station, Lake Maggiore, in 2011 obtained from counts of DAPI stained cells.
Mentions: Total abundance of prokaryotic cells (Bacteria and Archaea) displayed seasonal maxima of up to 7.3 × 106 cells mL−1 at 3 and 10 m depth concomitant with the spring (March-April) and summer (July) phytoplankton blooms. No seasonal trend was visible in the deep water layers (200 m and 350 m depth) where the overall abundance was generally lower than 1 × 106 cells mL−1 (Figure 2).

Bottom Line: In order to reach a high resolution at the Thaumarchaea community level, the probe MGI-535 was specifically designed for this study and applied to fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) analysis.We then applied it to a fine analysis of diversity and relative abundance of AOA in the deepest layers of the oligotrophic Lake Maggiore, confirming previous published results of AOA presence, but showing differences in abundance and distribution within the water column without significant seasonal trends with respect to Bacteria.Furthermore, phylogenetic analysis of AOA clone libraries from deep lake water and from a lake tributary, River Maggia, suggested the riverine origin of AOA of the deep hypolimnion of the lake.

View Article: PubMed Central - PubMed

Affiliation: Microbial Ecology Group, CNR-Institute of Ecosystem Study, Largo Tonolli 50, 28922 Verbania, Italy ; Microb&Co, Association for Microbial Ecology, Viale XX Settembre 45, 95128 Catania, Italy.

ABSTRACT
Ammonia-oxidizing Archaea (AOA) play an important role in the oxidation of ammonia in terrestrial, marine, and geothermal habitats, as confirmed by a number of studies specifically focused on those environments. Much less is known about the ecological role of AOA in freshwaters. In order to reach a high resolution at the Thaumarchaea community level, the probe MGI-535 was specifically designed for this study and applied to fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) analysis. We then applied it to a fine analysis of diversity and relative abundance of AOA in the deepest layers of the oligotrophic Lake Maggiore, confirming previous published results of AOA presence, but showing differences in abundance and distribution within the water column without significant seasonal trends with respect to Bacteria. Furthermore, phylogenetic analysis of AOA clone libraries from deep lake water and from a lake tributary, River Maggia, suggested the riverine origin of AOA of the deep hypolimnion of the lake.

Show MeSH