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The clinical significance of HER2 protein amplification/expression in urinary bladder lesion.

Hammam O, Nour HH, Mosaad M, Akl M, Khalil H, Al Ganzory H, Hindawi A - Arab J Urol (2015)

Bottom Line: HER2 expression was statistically significantly higher in patients with malignant lesions than in the other groups, and in high-stage and -grade tumours than in low-stage and -grade tumours.Schistosomal infestation did not affect HER2 expression in patients with transitional cell carcinoma.The treatment of HER2-positive tumours might benefit from novel targeted-treatment protocols.

View Article: PubMed Central - PubMed

Affiliation: Pathology Department, Theodor Bilharz Research Institute, Giza Egypt.

ABSTRACT

Objective: To evaluate HER2 oncoprotein expression by both immunohistochemical (IHC) staining and fluorescence in situ hybridisation (FISH) in different benign and malignant bladder lesions, and the effect of bilharzial infestation on this expression.

Patients and methods: In a prospective controlled study, 72 patients were classified into a control group, and groups with cystitis, urothelial carcinoma, and squamous cell carcinoma (SCC). HER2 was detected using standard IHC staining and FISH in all groups. The correlation of HER2 expression with tumour type, stage and grade in relation to normal urothelium and cystitis was assessed. The effect of schistosomal infestation was evaluated.

Results: HER2 expression was statistically significantly higher in patients with malignant lesions than in the other groups, and in high-stage and -grade tumours than in low-stage and -grade tumours. The use of FISH increased the detection of HER2-positive tumours. Schistosomal infestation did not affect HER2 expression in patients with transitional cell carcinoma.

Conclusion: High-stage and -grade bladder malignancies expressed HER2 much more than did benign lesions. FISH is more sensitive for detecting HER2 expression. The treatment of HER2-positive tumours might benefit from novel targeted-treatment protocols.

No MeSH data available.


Related in: MedlinePlus

IHC staining of the study group tissues. (A) A control showing negative HER2 protein, scoring 1+ in the urothelial lining. (B) A case of polypod cystitis showing negative HER2 protein immunoexpression, score 2+, IHC, Her2, DAB, ×200. (C) A case of poorly differentiated urothelial TCC, showing equivocal HER2 protein immunoexpression in >30% of tumour cells; IHC, Her2, DAB, ×400. (D) A case of papillary urothelial carcinoma showing strong HER2 protein immunoexpression, score 3+ in >70% of cells; IHC, Her2, DAB, ×200. (E) A case of moderately differentiated schistosomal-associated SCC, strongly positive for HER2 protein, score 3+; IHC, Her2, DAB, ×400. (F) A case of moderately differentiated SCC, grade II, showing negative HER2 protein immunoexpression, score 1+, IHC, Her2, DAB, ×200.
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f0005: IHC staining of the study group tissues. (A) A control showing negative HER2 protein, scoring 1+ in the urothelial lining. (B) A case of polypod cystitis showing negative HER2 protein immunoexpression, score 2+, IHC, Her2, DAB, ×200. (C) A case of poorly differentiated urothelial TCC, showing equivocal HER2 protein immunoexpression in >30% of tumour cells; IHC, Her2, DAB, ×400. (D) A case of papillary urothelial carcinoma showing strong HER2 protein immunoexpression, score 3+ in >70% of cells; IHC, Her2, DAB, ×200. (E) A case of moderately differentiated schistosomal-associated SCC, strongly positive for HER2 protein, score 3+; IHC, Her2, DAB, ×400. (F) A case of moderately differentiated SCC, grade II, showing negative HER2 protein immunoexpression, score 1+, IHC, Her2, DAB, ×200.

Mentions: HER2 protein was not expressed in both the control and the cystitis group (Fig. 1A and B), while there was strong expression in 31% (16/52) malignant cases (P < 0.01). Schistosomal infestation appeared not to affect the HER2 expression in patients with cystitis.


The clinical significance of HER2 protein amplification/expression in urinary bladder lesion.

Hammam O, Nour HH, Mosaad M, Akl M, Khalil H, Al Ganzory H, Hindawi A - Arab J Urol (2015)

IHC staining of the study group tissues. (A) A control showing negative HER2 protein, scoring 1+ in the urothelial lining. (B) A case of polypod cystitis showing negative HER2 protein immunoexpression, score 2+, IHC, Her2, DAB, ×200. (C) A case of poorly differentiated urothelial TCC, showing equivocal HER2 protein immunoexpression in >30% of tumour cells; IHC, Her2, DAB, ×400. (D) A case of papillary urothelial carcinoma showing strong HER2 protein immunoexpression, score 3+ in >70% of cells; IHC, Her2, DAB, ×200. (E) A case of moderately differentiated schistosomal-associated SCC, strongly positive for HER2 protein, score 3+; IHC, Her2, DAB, ×400. (F) A case of moderately differentiated SCC, grade II, showing negative HER2 protein immunoexpression, score 1+, IHC, Her2, DAB, ×200.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561918&req=5

f0005: IHC staining of the study group tissues. (A) A control showing negative HER2 protein, scoring 1+ in the urothelial lining. (B) A case of polypod cystitis showing negative HER2 protein immunoexpression, score 2+, IHC, Her2, DAB, ×200. (C) A case of poorly differentiated urothelial TCC, showing equivocal HER2 protein immunoexpression in >30% of tumour cells; IHC, Her2, DAB, ×400. (D) A case of papillary urothelial carcinoma showing strong HER2 protein immunoexpression, score 3+ in >70% of cells; IHC, Her2, DAB, ×200. (E) A case of moderately differentiated schistosomal-associated SCC, strongly positive for HER2 protein, score 3+; IHC, Her2, DAB, ×400. (F) A case of moderately differentiated SCC, grade II, showing negative HER2 protein immunoexpression, score 1+, IHC, Her2, DAB, ×200.
Mentions: HER2 protein was not expressed in both the control and the cystitis group (Fig. 1A and B), while there was strong expression in 31% (16/52) malignant cases (P < 0.01). Schistosomal infestation appeared not to affect the HER2 expression in patients with cystitis.

Bottom Line: HER2 expression was statistically significantly higher in patients with malignant lesions than in the other groups, and in high-stage and -grade tumours than in low-stage and -grade tumours.Schistosomal infestation did not affect HER2 expression in patients with transitional cell carcinoma.The treatment of HER2-positive tumours might benefit from novel targeted-treatment protocols.

View Article: PubMed Central - PubMed

Affiliation: Pathology Department, Theodor Bilharz Research Institute, Giza Egypt.

ABSTRACT

Objective: To evaluate HER2 oncoprotein expression by both immunohistochemical (IHC) staining and fluorescence in situ hybridisation (FISH) in different benign and malignant bladder lesions, and the effect of bilharzial infestation on this expression.

Patients and methods: In a prospective controlled study, 72 patients were classified into a control group, and groups with cystitis, urothelial carcinoma, and squamous cell carcinoma (SCC). HER2 was detected using standard IHC staining and FISH in all groups. The correlation of HER2 expression with tumour type, stage and grade in relation to normal urothelium and cystitis was assessed. The effect of schistosomal infestation was evaluated.

Results: HER2 expression was statistically significantly higher in patients with malignant lesions than in the other groups, and in high-stage and -grade tumours than in low-stage and -grade tumours. The use of FISH increased the detection of HER2-positive tumours. Schistosomal infestation did not affect HER2 expression in patients with transitional cell carcinoma.

Conclusion: High-stage and -grade bladder malignancies expressed HER2 much more than did benign lesions. FISH is more sensitive for detecting HER2 expression. The treatment of HER2-positive tumours might benefit from novel targeted-treatment protocols.

No MeSH data available.


Related in: MedlinePlus