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Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments.

Cheng Q, Aravind A, Buckley M, Gifford A, Parvin B - Sci Rep (2015)

Bottom Line: To date, in situ visualization of microbial density has remained an open problem.Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil.We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Biomedical Engineering, University of Nevada, Reno, 1664 N Virginia Street, Reno NV, 89503, USA.

ABSTRACT
To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

No MeSH data available.


Non-stickiness of C60-pyrrolidine tris-Cysteine to multiple soil matrices monitored by Ultraviolet light absorption.Recovery of C60-Cysteine from alumina (a), VWR sand (b), Wild Sand (c) and Natural Soil (d) of different mass indicate that the newly synthesized C60-pyrrolidine tris-Cysteine remains non-sticky to multiple matrices. The red regions indicate immediate recovery following incubation, and the green regions indicate recovery following multiple washes.
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f10: Non-stickiness of C60-pyrrolidine tris-Cysteine to multiple soil matrices monitored by Ultraviolet light absorption.Recovery of C60-Cysteine from alumina (a), VWR sand (b), Wild Sand (c) and Natural Soil (d) of different mass indicate that the newly synthesized C60-pyrrolidine tris-Cysteine remains non-sticky to multiple matrices. The red regions indicate immediate recovery following incubation, and the green regions indicate recovery following multiple washes.

Mentions: To validate non-stickiness to the matrix, both UV absorption and microscopy are utilized. Figure 10 shows a trend for recovering C60-pyrrolidine tris-cysteine from alumina (Fig. 10a), VWR sand (Fig. 10b), wild sand (Fig. 10c), and natural soil (Fig. 10d), all of which show results comparable to those previously reported (Fig. 5). More than 60% of C60-pyrrolidine tris-cysteine is removed from alumina, wild sand, and natural soil, without wash (red column). The rest of C60-pyrrolidine tris-cysteine is fully recovered with one additional H2O wash (green column).


Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments.

Cheng Q, Aravind A, Buckley M, Gifford A, Parvin B - Sci Rep (2015)

Non-stickiness of C60-pyrrolidine tris-Cysteine to multiple soil matrices monitored by Ultraviolet light absorption.Recovery of C60-Cysteine from alumina (a), VWR sand (b), Wild Sand (c) and Natural Soil (d) of different mass indicate that the newly synthesized C60-pyrrolidine tris-Cysteine remains non-sticky to multiple matrices. The red regions indicate immediate recovery following incubation, and the green regions indicate recovery following multiple washes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561912&req=5

f10: Non-stickiness of C60-pyrrolidine tris-Cysteine to multiple soil matrices monitored by Ultraviolet light absorption.Recovery of C60-Cysteine from alumina (a), VWR sand (b), Wild Sand (c) and Natural Soil (d) of different mass indicate that the newly synthesized C60-pyrrolidine tris-Cysteine remains non-sticky to multiple matrices. The red regions indicate immediate recovery following incubation, and the green regions indicate recovery following multiple washes.
Mentions: To validate non-stickiness to the matrix, both UV absorption and microscopy are utilized. Figure 10 shows a trend for recovering C60-pyrrolidine tris-cysteine from alumina (Fig. 10a), VWR sand (Fig. 10b), wild sand (Fig. 10c), and natural soil (Fig. 10d), all of which show results comparable to those previously reported (Fig. 5). More than 60% of C60-pyrrolidine tris-cysteine is removed from alumina, wild sand, and natural soil, without wash (red column). The rest of C60-pyrrolidine tris-cysteine is fully recovered with one additional H2O wash (green column).

Bottom Line: To date, in situ visualization of microbial density has remained an open problem.Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil.We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Biomedical Engineering, University of Nevada, Reno, 1664 N Virginia Street, Reno NV, 89503, USA.

ABSTRACT
To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

No MeSH data available.