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A Simple and Sensitive Method to Quantify Biodegradable Nanoparticle Biodistribution using Europium Chelates.

Crawford L, Higgins J, Putnam D - Sci Rep (2015)

Bottom Line: The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal.Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively.Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY.

ABSTRACT
The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from a lanthanide chelate to minimize background autofluorescence and maximize the signal to noise ratio to detect biodegradable nanoparticle distribution in mice. Specifically, antenna chelates containing europium were entrapped within nanoparticles composed of polylactic acid-polyethylene glycol diblock copolymers. Tissue accumulation of nanoparticles following intravenous injection was quantified in mice. The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal. Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively. Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

No MeSH data available.


Biodistribution of Eu(NTA)3 doped PLA-PEG nanoparticles in (a) liver, (b) kidney, (c) heart, (d) lung, (e) spleen, (f) brain, and (g) blood.
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f4: Biodistribution of Eu(NTA)3 doped PLA-PEG nanoparticles in (a) liver, (b) kidney, (c) heart, (d) lung, (e) spleen, (f) brain, and (g) blood.

Mentions: Using the second order degradation TFR curve and tissue interference factor to calculate the percent of injected NP in each tissue, biodistribution curves were produced for each tissue type. Particles were detectable above background in all tissues (Fig. 4). From the area under the curves (Table 2), the highest tissue accumulation for these PLA-PEG nanoparticles was in the spleen, followed by the liver and kidney. Particles were also present in the heart, lung and brain.


A Simple and Sensitive Method to Quantify Biodegradable Nanoparticle Biodistribution using Europium Chelates.

Crawford L, Higgins J, Putnam D - Sci Rep (2015)

Biodistribution of Eu(NTA)3 doped PLA-PEG nanoparticles in (a) liver, (b) kidney, (c) heart, (d) lung, (e) spleen, (f) brain, and (g) blood.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561907&req=5

f4: Biodistribution of Eu(NTA)3 doped PLA-PEG nanoparticles in (a) liver, (b) kidney, (c) heart, (d) lung, (e) spleen, (f) brain, and (g) blood.
Mentions: Using the second order degradation TFR curve and tissue interference factor to calculate the percent of injected NP in each tissue, biodistribution curves were produced for each tissue type. Particles were detectable above background in all tissues (Fig. 4). From the area under the curves (Table 2), the highest tissue accumulation for these PLA-PEG nanoparticles was in the spleen, followed by the liver and kidney. Particles were also present in the heart, lung and brain.

Bottom Line: The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal.Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively.Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY.

ABSTRACT
The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from a lanthanide chelate to minimize background autofluorescence and maximize the signal to noise ratio to detect biodegradable nanoparticle distribution in mice. Specifically, antenna chelates containing europium were entrapped within nanoparticles composed of polylactic acid-polyethylene glycol diblock copolymers. Tissue accumulation of nanoparticles following intravenous injection was quantified in mice. The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal. Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively. Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

No MeSH data available.