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A Simple and Sensitive Method to Quantify Biodegradable Nanoparticle Biodistribution using Europium Chelates.

Crawford L, Higgins J, Putnam D - Sci Rep (2015)

Bottom Line: The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal.Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively.Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY.

ABSTRACT
The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from a lanthanide chelate to minimize background autofluorescence and maximize the signal to noise ratio to detect biodegradable nanoparticle distribution in mice. Specifically, antenna chelates containing europium were entrapped within nanoparticles composed of polylactic acid-polyethylene glycol diblock copolymers. Tissue accumulation of nanoparticles following intravenous injection was quantified in mice. The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal. Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively. Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

No MeSH data available.


Fluorescent decrease of Eu(NTA)3 doped nanoparticles in plasma vs. time.(a) second order decrease in fluorescence. (b) manipulated inverse of second order decrease. Particles lose fluorescence due to the introduction of water into the chelate.
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f3: Fluorescent decrease of Eu(NTA)3 doped nanoparticles in plasma vs. time.(a) second order decrease in fluorescence. (b) manipulated inverse of second order decrease. Particles lose fluorescence due to the introduction of water into the chelate.

Mentions: The TRF signal of europium chelates is diminished on exposure to water17. To account for signal changes in the context of quantifying biodistribution, the TRF of europium-doped NPs was measured in plasma (Fig. 3). The signal decay was transformed into a linear form that signified a second-order process. A straight line fit provided an equation to assess signal degradation at any given time.


A Simple and Sensitive Method to Quantify Biodegradable Nanoparticle Biodistribution using Europium Chelates.

Crawford L, Higgins J, Putnam D - Sci Rep (2015)

Fluorescent decrease of Eu(NTA)3 doped nanoparticles in plasma vs. time.(a) second order decrease in fluorescence. (b) manipulated inverse of second order decrease. Particles lose fluorescence due to the introduction of water into the chelate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561907&req=5

f3: Fluorescent decrease of Eu(NTA)3 doped nanoparticles in plasma vs. time.(a) second order decrease in fluorescence. (b) manipulated inverse of second order decrease. Particles lose fluorescence due to the introduction of water into the chelate.
Mentions: The TRF signal of europium chelates is diminished on exposure to water17. To account for signal changes in the context of quantifying biodistribution, the TRF of europium-doped NPs was measured in plasma (Fig. 3). The signal decay was transformed into a linear form that signified a second-order process. A straight line fit provided an equation to assess signal degradation at any given time.

Bottom Line: The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal.Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively.Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY.

ABSTRACT
The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from a lanthanide chelate to minimize background autofluorescence and maximize the signal to noise ratio to detect biodegradable nanoparticle distribution in mice. Specifically, antenna chelates containing europium were entrapped within nanoparticles composed of polylactic acid-polyethylene glycol diblock copolymers. Tissue accumulation of nanoparticles following intravenous injection was quantified in mice. The TRF of the nanoparticles was found to diminish as a second order function in the presence of serum and tissue compositions interfered with the europium signal. Both phenomena were corrected by linearization of the signal function and calculation of tissue-specific interference, respectively. Overall, the method is simple and robust with a detection limit five times greater than standard fluorescent probes.

No MeSH data available.