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Microdissection of Human Esophagogastric Junction Wall with Phase-contrast X-ray CT Imaging.

Zhang J, Zhou G, Tian D, Lin R, Peng G, Su M - Sci Rep (2015)

Bottom Line: The shape of the mucous and muscular layers was intact.The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers.The surface of the esophagogastric junction was displayed clearly through 3D reconstruction.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, People's Republic of China.

ABSTRACT
Phase-contrast x-ray imaging using an x-ray interferometer has great potential to reveal the structures inside soft tissues, because the sensitivity of this method to hydrogen, carbon, nitrogen, and oxygen is about 1000 times higher than that of the absorption-contrast x-ray method. In this study, we used phase-contrast X-ray CT to investigate human resected esophagogastric junction. This technology revealed the three-layer structure of the esophagogastric junction wall-mucous, submucosa and muscular layers. The mucous and muscular layers were clearly separated by a loose submucosa layer with a honeycomb appearance. The shape of the mucous and muscular layers was intact. The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction. The technology might be helpful in the diagnosis of esophagogastric junction lesion, especially for the early adenocarcinoma.

No MeSH data available.


Related in: MedlinePlus

CT axial view image (A) coronal view image (B) Gas-barium double-contrast X-ray image (C) and sketch map (D) of the esophagogastric junction: (A,B) CT demonstrates the esophagogastric junction and the relation with adjacent tissues(the empty arrow). (C) Mucosa shows stellate high-density shadow (the empty arrow).
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f5: CT axial view image (A) coronal view image (B) Gas-barium double-contrast X-ray image (C) and sketch map (D) of the esophagogastric junction: (A,B) CT demonstrates the esophagogastric junction and the relation with adjacent tissues(the empty arrow). (C) Mucosa shows stellate high-density shadow (the empty arrow).

Mentions: Phase-contrast X-ray CT imaging by synchrotron-radiation broadband monochromatic light clearly depicted the normal esophagogastric junction wall, including the mucous, submucosa and muscular layers (Figs 2 and 3). The mucous and muscular layers were clearly separated by a loose submucosa layer, with a honeycomb appearance (Fig. 4). The shape of the mucous and muscular layers was complete. The boundary between the mucous and submucosa layers was distinct, and the same as the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction (Fig. 3). We found many strips of tubular low-density areas in the muscular layer, which were crevices that might be caused by fiber contraction during fixation or open-air drying (Fig. 4). For comparison, we show a gas-barium double contrast X-ray image and CT image of esophagogastric junction tissue from the same patients (Fig. 5). The gas-barium double contrast X-ray image showed the mucosa, displaying stellate high-density shadows (caused by the barium sulfate coating on the esophagogastric junction mucosa surface), as well as gas filling the esophagogastric junction lumen. CT revealed the esophagogastric junction wall in relation to adjacent tissues. However, both techniques combined could not discriminate the layers of the esophagogastric junction wall.


Microdissection of Human Esophagogastric Junction Wall with Phase-contrast X-ray CT Imaging.

Zhang J, Zhou G, Tian D, Lin R, Peng G, Su M - Sci Rep (2015)

CT axial view image (A) coronal view image (B) Gas-barium double-contrast X-ray image (C) and sketch map (D) of the esophagogastric junction: (A,B) CT demonstrates the esophagogastric junction and the relation with adjacent tissues(the empty arrow). (C) Mucosa shows stellate high-density shadow (the empty arrow).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561904&req=5

f5: CT axial view image (A) coronal view image (B) Gas-barium double-contrast X-ray image (C) and sketch map (D) of the esophagogastric junction: (A,B) CT demonstrates the esophagogastric junction and the relation with adjacent tissues(the empty arrow). (C) Mucosa shows stellate high-density shadow (the empty arrow).
Mentions: Phase-contrast X-ray CT imaging by synchrotron-radiation broadband monochromatic light clearly depicted the normal esophagogastric junction wall, including the mucous, submucosa and muscular layers (Figs 2 and 3). The mucous and muscular layers were clearly separated by a loose submucosa layer, with a honeycomb appearance (Fig. 4). The shape of the mucous and muscular layers was complete. The boundary between the mucous and submucosa layers was distinct, and the same as the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction (Fig. 3). We found many strips of tubular low-density areas in the muscular layer, which were crevices that might be caused by fiber contraction during fixation or open-air drying (Fig. 4). For comparison, we show a gas-barium double contrast X-ray image and CT image of esophagogastric junction tissue from the same patients (Fig. 5). The gas-barium double contrast X-ray image showed the mucosa, displaying stellate high-density shadows (caused by the barium sulfate coating on the esophagogastric junction mucosa surface), as well as gas filling the esophagogastric junction lumen. CT revealed the esophagogastric junction wall in relation to adjacent tissues. However, both techniques combined could not discriminate the layers of the esophagogastric junction wall.

Bottom Line: The shape of the mucous and muscular layers was intact.The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers.The surface of the esophagogastric junction was displayed clearly through 3D reconstruction.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, People's Republic of China.

ABSTRACT
Phase-contrast x-ray imaging using an x-ray interferometer has great potential to reveal the structures inside soft tissues, because the sensitivity of this method to hydrogen, carbon, nitrogen, and oxygen is about 1000 times higher than that of the absorption-contrast x-ray method. In this study, we used phase-contrast X-ray CT to investigate human resected esophagogastric junction. This technology revealed the three-layer structure of the esophagogastric junction wall-mucous, submucosa and muscular layers. The mucous and muscular layers were clearly separated by a loose submucosa layer with a honeycomb appearance. The shape of the mucous and muscular layers was intact. The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction. The technology might be helpful in the diagnosis of esophagogastric junction lesion, especially for the early adenocarcinoma.

No MeSH data available.


Related in: MedlinePlus