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Microdissection of Human Esophagogastric Junction Wall with Phase-contrast X-ray CT Imaging.

Zhang J, Zhou G, Tian D, Lin R, Peng G, Su M - Sci Rep (2015)

Bottom Line: The shape of the mucous and muscular layers was intact.The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers.The surface of the esophagogastric junction was displayed clearly through 3D reconstruction.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, People's Republic of China.

ABSTRACT
Phase-contrast x-ray imaging using an x-ray interferometer has great potential to reveal the structures inside soft tissues, because the sensitivity of this method to hydrogen, carbon, nitrogen, and oxygen is about 1000 times higher than that of the absorption-contrast x-ray method. In this study, we used phase-contrast X-ray CT to investigate human resected esophagogastric junction. This technology revealed the three-layer structure of the esophagogastric junction wall-mucous, submucosa and muscular layers. The mucous and muscular layers were clearly separated by a loose submucosa layer with a honeycomb appearance. The shape of the mucous and muscular layers was intact. The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction. The technology might be helpful in the diagnosis of esophagogastric junction lesion, especially for the early adenocarcinoma.

No MeSH data available.


Related in: MedlinePlus

The picture of BL13W1 beamline partial facility of SSRF.1. A multidimensional specimen table. The specimens are placed on the specimen table to rotate and specimens were obtained in different angles. 2. An X-ray CCD. It obtained specimens’ projective images with high-resolution. 3. The precise guide rail. It can control the exact distance from the CCD to the specimens.
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f1: The picture of BL13W1 beamline partial facility of SSRF.1. A multidimensional specimen table. The specimens are placed on the specimen table to rotate and specimens were obtained in different angles. 2. An X-ray CCD. It obtained specimens’ projective images with high-resolution. 3. The precise guide rail. It can control the exact distance from the CCD to the specimens.

Mentions: The principle of synchrotron-radiation X-ray phase-contrast imaging is based on Fresnel diffraction theory. The experiment setup, when using a synchrotron radiation light source, is simple, requiring only a double-crystal monochromator, a sample stage and a detector. Figure 1 shows the real photo of the experiment setup1518.


Microdissection of Human Esophagogastric Junction Wall with Phase-contrast X-ray CT Imaging.

Zhang J, Zhou G, Tian D, Lin R, Peng G, Su M - Sci Rep (2015)

The picture of BL13W1 beamline partial facility of SSRF.1. A multidimensional specimen table. The specimens are placed on the specimen table to rotate and specimens were obtained in different angles. 2. An X-ray CCD. It obtained specimens’ projective images with high-resolution. 3. The precise guide rail. It can control the exact distance from the CCD to the specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561904&req=5

f1: The picture of BL13W1 beamline partial facility of SSRF.1. A multidimensional specimen table. The specimens are placed on the specimen table to rotate and specimens were obtained in different angles. 2. An X-ray CCD. It obtained specimens’ projective images with high-resolution. 3. The precise guide rail. It can control the exact distance from the CCD to the specimens.
Mentions: The principle of synchrotron-radiation X-ray phase-contrast imaging is based on Fresnel diffraction theory. The experiment setup, when using a synchrotron radiation light source, is simple, requiring only a double-crystal monochromator, a sample stage and a detector. Figure 1 shows the real photo of the experiment setup1518.

Bottom Line: The shape of the mucous and muscular layers was intact.The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers.The surface of the esophagogastric junction was displayed clearly through 3D reconstruction.

View Article: PubMed Central - PubMed

Affiliation: First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, People's Republic of China.

ABSTRACT
Phase-contrast x-ray imaging using an x-ray interferometer has great potential to reveal the structures inside soft tissues, because the sensitivity of this method to hydrogen, carbon, nitrogen, and oxygen is about 1000 times higher than that of the absorption-contrast x-ray method. In this study, we used phase-contrast X-ray CT to investigate human resected esophagogastric junction. This technology revealed the three-layer structure of the esophagogastric junction wall-mucous, submucosa and muscular layers. The mucous and muscular layers were clearly separated by a loose submucosa layer with a honeycomb appearance. The shape of the mucous and muscular layers was intact. The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction. The technology might be helpful in the diagnosis of esophagogastric junction lesion, especially for the early adenocarcinoma.

No MeSH data available.


Related in: MedlinePlus