Limits...
Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

The number of viable bacteria from culture plates treated using different pH values(a), Mg2+ concentrations (b), and osmolality values (c). At each time point, columns labelled with the same letter are not significantly different (p > 0.05) but columns labelled with different letters are significantly different (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4561899&req=5

f7: The number of viable bacteria from culture plates treated using different pH values(a), Mg2+ concentrations (b), and osmolality values (c). At each time point, columns labelled with the same letter are not significantly different (p > 0.05) but columns labelled with different letters are significantly different (p < 0.05).

Mentions: Figure 7 presents the results of the effect of different pH values, Mg ion concentrations, and osmolality values on the bacterial activity. Figure 7a shows that different pH values had an influence on the bacterial activity. When the pH was 8.5, the number of bacteria clearly decreased compared to pH 7.4 or 8.0 (p < 0.05); when the pH reached 9.0 or 9.5, the anti-bacterial effect was more pronounced compared to pH 7.4, 8.0, or 8.5 (p < 0.01). Figure 7b shows that there was no significant difference between different ionic concentrations of Mg (p > 0.05), implying that a certain range of ionic concentrations of Mg (0–0.20 mmol/L) did not affect the vitality of bacteria. Similarly, no significant difference was found between different osmolalities (p > 0.05)(Fig. 7c), indicating that osmolality within a certain range (less than 500 mOsmol/kg) had no influence on the bacterial activity.


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

The number of viable bacteria from culture plates treated using different pH values(a), Mg2+ concentrations (b), and osmolality values (c). At each time point, columns labelled with the same letter are not significantly different (p > 0.05) but columns labelled with different letters are significantly different (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f7: The number of viable bacteria from culture plates treated using different pH values(a), Mg2+ concentrations (b), and osmolality values (c). At each time point, columns labelled with the same letter are not significantly different (p > 0.05) but columns labelled with different letters are significantly different (p < 0.05).
Mentions: Figure 7 presents the results of the effect of different pH values, Mg ion concentrations, and osmolality values on the bacterial activity. Figure 7a shows that different pH values had an influence on the bacterial activity. When the pH was 8.5, the number of bacteria clearly decreased compared to pH 7.4 or 8.0 (p < 0.05); when the pH reached 9.0 or 9.5, the anti-bacterial effect was more pronounced compared to pH 7.4, 8.0, or 8.5 (p < 0.01). Figure 7b shows that there was no significant difference between different ionic concentrations of Mg (p > 0.05), implying that a certain range of ionic concentrations of Mg (0–0.20 mmol/L) did not affect the vitality of bacteria. Similarly, no significant difference was found between different osmolalities (p > 0.05)(Fig. 7c), indicating that osmolality within a certain range (less than 500 mOsmol/kg) had no influence on the bacterial activity.

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus