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Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

Degradation features of the PLGA/TCP/Mg scaffolds:(a) Changes in pH of the degradation medium; (b) Changes in ionic concentration of Mg2+; (c) Changes in osmolality.
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f6: Degradation features of the PLGA/TCP/Mg scaffolds:(a) Changes in pH of the degradation medium; (b) Changes in ionic concentration of Mg2+; (c) Changes in osmolality.

Mentions: The results of the in vitro degradation tests are shown in Fig. 6. The pH of the PT group was less than 7.4 along with the degradation of the PT scaffold within 168 hours (Fig. 6a). In contrast, the pH values of all three Mg groups (PT5M, PT10M, and PT15M) were greater than 7.4 within 168 hours, greater than 8.5 within 48 hours, and greater than 9 within 24 hours. The pH of PT15M was obviously higher than those of PT10M at 12, 48, and 120 hours (p < 0.05) and higher than those of PT5M at each time point within 120 hours (p < 0.05). The pH values of PT10M was clearly higher than those of PT5M at 6 and 48 hours (p < 0.05). All Mg groups released Mg2+ into the extracts, leading to a gradual increase in the Mg2+ concentration within 168 hours (Fig. 6b). The degradation of the PT group caused no changes in the osmolality (Fig. 6c). However, the osmolalities of the three Mg groups increased within 48 hours, and comparison among them showed PT15M > PT10M > PT5M at each time point (p < 0.05).


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Degradation features of the PLGA/TCP/Mg scaffolds:(a) Changes in pH of the degradation medium; (b) Changes in ionic concentration of Mg2+; (c) Changes in osmolality.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f6: Degradation features of the PLGA/TCP/Mg scaffolds:(a) Changes in pH of the degradation medium; (b) Changes in ionic concentration of Mg2+; (c) Changes in osmolality.
Mentions: The results of the in vitro degradation tests are shown in Fig. 6. The pH of the PT group was less than 7.4 along with the degradation of the PT scaffold within 168 hours (Fig. 6a). In contrast, the pH values of all three Mg groups (PT5M, PT10M, and PT15M) were greater than 7.4 within 168 hours, greater than 8.5 within 48 hours, and greater than 9 within 24 hours. The pH of PT15M was obviously higher than those of PT10M at 12, 48, and 120 hours (p < 0.05) and higher than those of PT5M at each time point within 120 hours (p < 0.05). The pH values of PT10M was clearly higher than those of PT5M at 6 and 48 hours (p < 0.05). All Mg groups released Mg2+ into the extracts, leading to a gradual increase in the Mg2+ concentration within 168 hours (Fig. 6b). The degradation of the PT group caused no changes in the osmolality (Fig. 6c). However, the osmolalities of the three Mg groups increased within 48 hours, and comparison among them showed PT15M > PT10M > PT5M at each time point (p < 0.05).

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus