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Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

CLSM images showing live and dead bacteria in different groups.Live cells appeared green and dead cells appeared red under CLSM. Scale bar is 50 μm.
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f5: CLSM images showing live and dead bacteria in different groups.Live cells appeared green and dead cells appeared red under CLSM. Scale bar is 50 μm.

Mentions: In Fig. 5, which presents CLSM images, live bacteria appeared with green fluorescence and dead ones appeared with red fluorescence. At 6 hours, considerably less green fluorescence could be observed on the PT5M, PT10M and PT15M groups (Fig. 5a2,a3,a4) than on the PT group (Fig. 5a1), indicating that less live bacteria adhered on PT5M, PT10M and PT15M than on PT. An extraordinarily dense green fluorescence indicating bacterial colonization could be observed on the PT group at 24 (Fig. 5b1) and 48 hours (Fig. 5c1). However, the green fluorescence was dispersed on PT5M, PT10M and PT15M (Fig. 5b2–b4,c2–c4), indicating no biofilm formation. Comparing the fluorescence denseness of the three Mg groups (PT5M, PT10M and PT15M), it could be observed that the red fluorescence was denser on PT10M and PT15M than on PT5M at 24 and 48 hours, and the green fluorescence was sparser on PT10M and PT15M than on PT5M at 48 hours. In other words, more dead bacteria and less live bacteria were present on PT10M and PT15M than on PT5M at 24 and 48 hours.


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

CLSM images showing live and dead bacteria in different groups.Live cells appeared green and dead cells appeared red under CLSM. Scale bar is 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f5: CLSM images showing live and dead bacteria in different groups.Live cells appeared green and dead cells appeared red under CLSM. Scale bar is 50 μm.
Mentions: In Fig. 5, which presents CLSM images, live bacteria appeared with green fluorescence and dead ones appeared with red fluorescence. At 6 hours, considerably less green fluorescence could be observed on the PT5M, PT10M and PT15M groups (Fig. 5a2,a3,a4) than on the PT group (Fig. 5a1), indicating that less live bacteria adhered on PT5M, PT10M and PT15M than on PT. An extraordinarily dense green fluorescence indicating bacterial colonization could be observed on the PT group at 24 (Fig. 5b1) and 48 hours (Fig. 5c1). However, the green fluorescence was dispersed on PT5M, PT10M and PT15M (Fig. 5b2–b4,c2–c4), indicating no biofilm formation. Comparing the fluorescence denseness of the three Mg groups (PT5M, PT10M and PT15M), it could be observed that the red fluorescence was denser on PT10M and PT15M than on PT5M at 24 and 48 hours, and the green fluorescence was sparser on PT10M and PT15M than on PT5M at 48 hours. In other words, more dead bacteria and less live bacteria were present on PT10M and PT15M than on PT5M at 24 and 48 hours.

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus