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Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

Representative SEM images showing bacterial adhesion and biofilm formation on surfaces of specimens.
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f4: Representative SEM images showing bacterial adhesion and biofilm formation on surfaces of specimens.

Mentions: As shown in Fig. 4, any adherent bacteria or formed biofilms could be observed in the SEM images. At 6 hours, there was a mass of clustered bacteria in the PT group (Fig. 4a1,b1); in comparison, fewer bacteria were observed in the PT5M group (Fig. 4a2,b2), PT10M group (Fig. 4a3), and PT15M group (Fig. 4a4,b4) relative to the PT group. After incubation for 24 hours, fewer scattered single colonies were observed in the PT5M (Fig. 4c2), PT10M (Fig. 4c3) and PT15M (Fig. 4c4) groups, particularly in the PT15M group; however, a biofilm was observed, which was formed by a large number of multiple bacteria colonies in the PT group (Fig. 4c1). At 48 hours, a thick layer of biofilm formed in the PT group (Fig. 4d1), and some large clustered bacterial colonies formed in the PT5M group (Fig. 4d2); in contrast, a few disperse bacterial colonies were present in the PT10M (Fig. 4d3) and PT15M groups (Fig. 4d4). As the incubation time increased (from 6 to 48 hours), bacteria increasingly grew on the PT surface with an apparent biofilm formed at 24 and 48 hours, but this trend was not apparent in the PT10M and PT15M groups.


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Representative SEM images showing bacterial adhesion and biofilm formation on surfaces of specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f4: Representative SEM images showing bacterial adhesion and biofilm formation on surfaces of specimens.
Mentions: As shown in Fig. 4, any adherent bacteria or formed biofilms could be observed in the SEM images. At 6 hours, there was a mass of clustered bacteria in the PT group (Fig. 4a1,b1); in comparison, fewer bacteria were observed in the PT5M group (Fig. 4a2,b2), PT10M group (Fig. 4a3), and PT15M group (Fig. 4a4,b4) relative to the PT group. After incubation for 24 hours, fewer scattered single colonies were observed in the PT5M (Fig. 4c2), PT10M (Fig. 4c3) and PT15M (Fig. 4c4) groups, particularly in the PT15M group; however, a biofilm was observed, which was formed by a large number of multiple bacteria colonies in the PT group (Fig. 4c1). At 48 hours, a thick layer of biofilm formed in the PT group (Fig. 4d1), and some large clustered bacterial colonies formed in the PT5M group (Fig. 4d2); in contrast, a few disperse bacterial colonies were present in the PT10M (Fig. 4d3) and PT15M groups (Fig. 4d4). As the incubation time increased (from 6 to 48 hours), bacteria increasingly grew on the PT surface with an apparent biofilm formed at 24 and 48 hours, but this trend was not apparent in the PT10M and PT15M groups.

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus