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Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

Biofilm formation assay as determined using the TCP method.**denotes a significant difference compared to the PT group (p < 0.01); ##denotes a significant difference compared to the PT5M group (p < 0.01).
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f3: Biofilm formation assay as determined using the TCP method.**denotes a significant difference compared to the PT group (p < 0.01); ##denotes a significant difference compared to the PT5M group (p < 0.01).

Mentions: The results of biofilm formation assessed using the TCP method are presented in Fig. 3. The OD values of the PT5M, PT10M and PT15M groups were significantly lower than that of the PT group at 24 and 48 hours (p < 0.01). The OD values of the PT10M and PT15M groups were clearly lower than that of the PT5M group at 48 hours (p < 0.01), which was consistent with the results determined using the spread plate method (Fig. 2b). In addition, no difference was observed between the PT10M group and the PT15M group at each time point (p > 0.05).


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Biofilm formation assay as determined using the TCP method.**denotes a significant difference compared to the PT group (p < 0.01); ##denotes a significant difference compared to the PT5M group (p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f3: Biofilm formation assay as determined using the TCP method.**denotes a significant difference compared to the PT group (p < 0.01); ##denotes a significant difference compared to the PT5M group (p < 0.01).
Mentions: The results of biofilm formation assessed using the TCP method are presented in Fig. 3. The OD values of the PT5M, PT10M and PT15M groups were significantly lower than that of the PT group at 24 and 48 hours (p < 0.01). The OD values of the PT10M and PT15M groups were clearly lower than that of the PT5M group at 48 hours (p < 0.01), which was consistent with the results determined using the spread plate method (Fig. 2b). In addition, no difference was observed between the PT10M group and the PT15M group at each time point (p > 0.05).

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus