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Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

The number of viable bacteria on the surface of different specimens at 24 and 48 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies on the surfaces of different specimens; (b) Quantitative analysis of viable bacteria. The numbers of colonies were counted and normalized to the counts from the PT group at 24 hours. **denotes a significant difference compared to the PT group (p < 0.01); #denotes a significant difference compared to the PT5M group (#p < 0.05; ##p < 0.01).
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f2: The number of viable bacteria on the surface of different specimens at 24 and 48 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies on the surfaces of different specimens; (b) Quantitative analysis of viable bacteria. The numbers of colonies were counted and normalized to the counts from the PT group at 24 hours. **denotes a significant difference compared to the PT group (p < 0.01); #denotes a significant difference compared to the PT5M group (#p < 0.05; ##p < 0.01).

Mentions: Figure 2 presents the results of the bacterial growth assay using the spread plate method. As shown in Fig. 2a, there was a small number of colonies on the Mg groups (PT5M, PT10M, and PT15M) at 24 and 48 hours; in contrast, a large number of colonies was present on PT. Quantitatively, the CFUs of the Mg groups were significantly less than that of the PT group at each time point (p < 0.01, Fig. 2b). Considering log-reduction with respect to PT, the bacteria on PT, PT5M and PT15M reduced 0.95-log, 1.45-log and 1.65-log at 24 hours, while at 48 hours the bacteria on PT, PT5M and PT15M reduced 0.72-log, 1.15-log and 1.22-log, respectively (Table 1). Comparing between the three Mg groups, the numbers of colonies on the PT10M and PT15M groups were significantly lower than that on the PT5M group at 24 and 48 hours (p < 0.05, Fig. 2b).


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

The number of viable bacteria on the surface of different specimens at 24 and 48 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies on the surfaces of different specimens; (b) Quantitative analysis of viable bacteria. The numbers of colonies were counted and normalized to the counts from the PT group at 24 hours. **denotes a significant difference compared to the PT group (p < 0.01); #denotes a significant difference compared to the PT5M group (#p < 0.05; ##p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f2: The number of viable bacteria on the surface of different specimens at 24 and 48 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies on the surfaces of different specimens; (b) Quantitative analysis of viable bacteria. The numbers of colonies were counted and normalized to the counts from the PT group at 24 hours. **denotes a significant difference compared to the PT group (p < 0.01); #denotes a significant difference compared to the PT5M group (#p < 0.05; ##p < 0.01).
Mentions: Figure 2 presents the results of the bacterial growth assay using the spread plate method. As shown in Fig. 2a, there was a small number of colonies on the Mg groups (PT5M, PT10M, and PT15M) at 24 and 48 hours; in contrast, a large number of colonies was present on PT. Quantitatively, the CFUs of the Mg groups were significantly less than that of the PT group at each time point (p < 0.01, Fig. 2b). Considering log-reduction with respect to PT, the bacteria on PT, PT5M and PT15M reduced 0.95-log, 1.45-log and 1.65-log at 24 hours, while at 48 hours the bacteria on PT, PT5M and PT15M reduced 0.72-log, 1.15-log and 1.22-log, respectively (Table 1). Comparing between the three Mg groups, the numbers of colonies on the PT10M and PT15M groups were significantly lower than that on the PT5M group at 24 and 48 hours (p < 0.05, Fig. 2b).

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus