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Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus

Bacterial adhesion at 6 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies from adhered bacteria; (b) Quantitative analysis of bacterial adhesion. The numbers of viable bacteria were counted and normalized to the counts from the PT group. ** denotes a significant difference compared to the PT group (p < 0.01).
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f1: Bacterial adhesion at 6 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies from adhered bacteria; (b) Quantitative analysis of bacterial adhesion. The numbers of viable bacteria were counted and normalized to the counts from the PT group. ** denotes a significant difference compared to the PT group (p < 0.01).

Mentions: Figure 1 presents the results of the bacterial adhesion assay using the spread plate method. Colonies were found less in the Mg groups (PT5M, PT10M, and PT15M) compared with the PT group (Fig. 1a). The bacterial adhesion was quantitatively determined by counting the numbers of colonies on TSA, as shown in Fig. 1b. The CFUs on PT5M, PT10M and PT15M (normalized by PT) were 24.2%, 16.1% and 10.6%, respectively, which are significantly less than that of PT (p < 0.01 for all). With respect to PT, the bacterial on PT5M, PT10M and PT15M reduced 0.62-log, 0.79-log and 0.97-log, respectively (Table 1).


Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds--an in vitro efficacy study.

Ma R, Lai YX, Li L, Tan HL, Wang JL, Li Y, Tang TT, Qin L - Sci Rep (2015)

Bacterial adhesion at 6 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies from adhered bacteria; (b) Quantitative analysis of bacterial adhesion. The numbers of viable bacteria were counted and normalized to the counts from the PT group. ** denotes a significant difference compared to the PT group (p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561899&req=5

f1: Bacterial adhesion at 6 hours as determined using the spread plate method:(a) Representative images of TSA with bacterial colonies from adhered bacteria; (b) Quantitative analysis of bacterial adhesion. The numbers of viable bacteria were counted and normalized to the counts from the PT group. ** denotes a significant difference compared to the PT group (p < 0.01).
Mentions: Figure 1 presents the results of the bacterial adhesion assay using the spread plate method. Colonies were found less in the Mg groups (PT5M, PT10M, and PT15M) compared with the PT group (Fig. 1a). The bacterial adhesion was quantitatively determined by counting the numbers of colonies on TSA, as shown in Fig. 1b. The CFUs on PT5M, PT10M and PT15M (normalized by PT) were 24.2%, 16.1% and 10.6%, respectively, which are significantly less than that of PT (p < 0.01 for all). With respect to PT, the bacterial on PT5M, PT10M and PT15M reduced 0.62-log, 0.79-log and 0.97-log, respectively (Table 1).

Bottom Line: A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M).In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds.In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

ABSTRACT
Bone infections are common in trauma-induced open fractures with bone defects. Therefore, developing anti-infection scaffolds for repairing bone defects is desirable. This study develoepd novel Mg-based porous composite scaffolds with a basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium phosphate (TCP). A unique low-temperature rapid prototyping technology was used to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and biofilm formation of Staphylococcus aureus were evaluated. The results indicated that the Mg-based scaffolds significantly inhibited bacterial adhesion and biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) concentration and osmolality, and the increased pH may be one of the major contributing factors to the antibacterial function of the Mg-based scaffolds. Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was considered to be an effective composition with considerable antibacterial ability and good cytocompatibility.

No MeSH data available.


Related in: MedlinePlus